PMID- 34591908
OWN - NLM
STAT- MEDLINE
DCOM- 20211122
LR  - 20211122
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 16
IP  - 9
DP  - 2021
TI  - Chromosomal variations of Lycoris species revealed by FISH with rDNAs and 
      centromeric histone H3 variant associated DNAs.
PG  - e0258028
LID - 10.1371/journal.pone.0258028 [doi]
LID - e0258028
AB  - Lycoris species have various chromosome numbers and karyotypes, but all have a 
      constant total number of chromosome major arms. In addition to three fundamental 
      types, including metacentric (M-), telocentric (T-), and acrocentric (A-) 
      chromosomes, chromosomes in various morphology and size were also observed in 
      natural populations. Both fusion and fission translocation have been considered 
      as main mechanisms leading to the diverse karyotypes among Lycoris species, which 
      suggests the centromere organization playing a role in such arrangements. We 
      detected several chromosomal structure changes in Lycoris including centric 
      fusion, inversion, gene amplification, and segment deletion by using fluorescence 
      in situ hybridization (FISH) probing with rDNAs. An antibody against centromere 
      specific histone H3 (CENH3) of L. aurea (2n = 14, 8M+6T) was raised and used to 
      obtain CENH3-associated DNA sequences of L. aurea by chromatin 
      immunoprecipitation (ChIP) cloning method. Immunostaining with anti-CENH3 
      antibody could label the centromeres of M-, T-, and A-type chromosomes. 
      Immunostaining also revealed two centromeres on one T-type chromosome and a 
      centromere on individual mini-chromosome. Among 10,000 ChIP clones, 500 clones 
      which showed abundant in L. aurea genome by dot-blotting analysis were FISH 
      mapped on chromosomes to examine their cytological distribution. Five of these 
      500 clones could generate intense FISH signals at centromeric region on M-type 
      but not T-type chromosomes. FISH signals of these five clones rarely appeared on 
      A-type chromosomes. The five ChIP clones showed similarity in DNA sequences and 
      could generate similar but not identical distribution patterns of FISH signals on 
      individual chromosomes. Furthermore, the distinct distribution patterns of FISH 
      signals on each chromosome generated by these five ChIP clones allow to identify 
      individual chromosome, which is considered difficult by conventional staining 
      approaches. Our results suggest a different organization of centromeres of the 
      three chromosome types in Lycoris species.
FAU - Liu, Mao-Sen
AU  - Liu MS
AD  - Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan.
FAU - Tseng, Shih-Hsuan
AU  - Tseng SH
AD  - Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan.
FAU - Tsai, Ching-Chi
AU  - Tsai CC
AD  - Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan.
FAU - Chen, Ting-Chu
AU  - Chen TC
AD  - Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan.
FAU - Chung, Mei-Chu
AU  - Chung MC
AUID- ORCID: 0000-0002-2946-7929
AD  - Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210930
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (DNA, Ribosomal)
RN  - 0 (Histones)
SB  - IM
MH  - *Centromere
MH  - Chromatin Immunoprecipitation
MH  - *Chromosomes, Plant
MH  - *DNA, Ribosomal
MH  - Gene Amplification
MH  - Gene Deletion
MH  - Histones/*genetics/metabolism
MH  - In Situ Hybridization, Fluorescence
MH  - Karyotype
MH  - Lycoris/*genetics/metabolism
PMC - PMC8483392
COIS- The author, Ching-Chi Tsai is currently employed by CH Biotech R & D Co., LTD 
      (CHB); but, before she moved to CHB, she worked as a post-doctor and joined this 
      study which was supported by the funding from the Institute of Plant and 
      Microbial Biology, Academia Sinica, Taiwan, ROC. That commercial company, CH 
      Biotech R & D Co., LTD, did not play any role in this study. Therefore, the 
      author's affiliation is "Institute of Plant and Microbial Biology, Academia 
      Sinica, Taipei, Taiwan", and "CH Biotech R & D Co., LTD "is her current address. 
      The authors have declared that no competing interests exist.
EDAT- 2021/10/01 06:00
MHDA- 2021/11/23 06:00
PMCR- 2021/09/30
CRDT- 2021/09/30 17:27
PHST- 2021/06/13 00:00 [received]
PHST- 2021/09/17 00:00 [accepted]
PHST- 2021/09/30 17:27 [entrez]
PHST- 2021/10/01 06:00 [pubmed]
PHST- 2021/11/23 06:00 [medline]
PHST- 2021/09/30 00:00 [pmc-release]
AID - PONE-D-21-19380 [pii]
AID - 10.1371/journal.pone.0258028 [doi]
PST - epublish
SO  - PLoS One. 2021 Sep 30;16(9):e0258028. doi: 10.1371/journal.pone.0258028. 
      eCollection 2021.