PMID- 34593425
OWN - NLM
STAT- MEDLINE
DCOM- 20211008
LR  - 20211008
IS  - 1791-7530 (Electronic)
IS  - 0250-7005 (Linking)
VI  - 41
IP  - 10
DP  - 2021 Oct
TI  - Anti-inflammatory and Anti-thrombotic Efficacy of Targeted Ultrasound 
      Microbubbles on LPS-induced HUVEC Cells.
PG  - 4761-4769
LID - 10.21873/anticanres.15291 [doi]
AB  - BACKGROUND/AIM: The early stage of atherosclerosis (AS) demonstrates a 
      lipid-driven inflammatory cytokine increase. In the present study, we aimed to 
      use ultrasound-targeted microbubble delivery (UTMD) therapy with the 
      Endostar-loaded target microbubbles (MBs) to reduce AS-related inflammatory 
      response. MATERIALS AND METHODS: Normal and lipopolysaccharide (LPS) induced 
      human umbilical vein endothelial cells (HUVECs) were placed in a parallel-plate 
      flow chamber. MBs were perfused through the parallel-plate flow chamber to mimic 
      physiological blood flow. Five groups were set up: G1: Negative control (normal 
      HUVECs); G2: LPS control (LPS induced HUVECs); G3: ICAM-1-loaded-MBs (MBi); G4: 
      Endostar-loaded-MBs (MBe) and G5: Endostar-ICAM-1-loaded-MBs (MBei). mRNA 
      expression of inflammatory factors and release of inflammatory cytokines were 
      detected by RT-PCR and ELISA, respectively. RESULTS: After treatment with MBei, 
      the mRNA expression of cell adhesion molecule-1 (CD31) (p=0.004), endothelin-1 
      (ET-1) (p=0.010), von willebrand factor (vWF) (p=0.018), extracellular regulated 
      protein kinases (ERK) (p=0.046) and nuclear factor kappa B (NF-kappaB) (p=0.003) were 
      significantly reduced compared to LPS-induced HUVECs. Release of inflammatory 
      cytokines including tissue factor (TF) (p=0.033), tissue factor pathway inhibitor 
      (TF-PI) (p=0.019), ET-1 (p=0.014), vWF (p=0.030) and blood-coagulation factor 
      VIIalpha (FVIIalpha) (p=0.000) were also significantly reduced compared to LPS-induced 
      HUVECs. CONCLUSION: UTMD therapy can inhibit the inflammatory response by 
      reducing atherosclerotic-related inflammatory factors, suggesting a potential 
      treatment at the early-stage of AS.
CI  - Copyright (c) 2021 International Institute of Anticancer Research (Dr. George J. 
      Delinasios), All rights reserved.
FAU - Sun, Jindong
AU  - Sun J
AD  - First People's Hospital of Linping District, Hangzhou, P.R. China.
FAU - Pan, Shijun
AU  - Pan S
AD  - Hangzhou Normal University, Hangzhou, P.R. China.
FAU - Yu, Huamin
AU  - Yu H
AD  - First People's Hospital of Linping District, Hangzhou, P.R. China.
FAU - Hu, Haiqiang
AU  - Hu H
AD  - First People's Hospital of Linping District, Hangzhou, P.R. China.
FAU - Sun, Y U
AU  - Sun YU
AD  - Department of Surgery, UCSD, San Diego, CA, U.S.A.
FAU - Yang, Zhijian
AU  - Yang Z
AD  - AntiCancer Inc., San Diego, CA, U.S.A.
FAU - Hoffman, Robert M
AU  - Hoffman RM
AD  - AntiCancer Inc., San Diego, CA, U.S.A.
FAU - Yuan, Hong
AU  - Yuan H
AD  - First People's Hospital of Linping District, Hangzhou, P.R. China; 
      halloween0708@163.com.
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Endostatins)
RN  - 0 (Fibrinolytic Agents)
RN  - 0 (Inflammation Mediators)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Recombinant Proteins)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - GVG18ZDN65 (endostar protein)
SB  - IM
MH  - Anti-Inflammatory Agents/chemistry/*pharmacology
MH  - Atherosclerosis/chemically induced/metabolism/therapy
MH  - Cell Adhesion
MH  - Endostatins/chemistry/pharmacology
MH  - Fibrinolytic Agents/chemistry/*pharmacology
MH  - Human Umbilical Vein Endothelial Cells/*drug effects/metabolism
MH  - Humans
MH  - Inflammation Mediators/metabolism
MH  - Intercellular Adhesion Molecule-1/immunology/metabolism
MH  - Lipopolysaccharides/toxicity
MH  - *Microbubbles
MH  - Recombinant Proteins/chemistry/pharmacology
MH  - Ultrasonics
OTO - NOTNLM
OT  - Atherosclerosis
OT  - LPS-induced HUVECs
OT  - inflammatory cytokines
OT  - parallel-plate flow chamber
OT  - ultrasound microbubbles
EDAT- 2021/10/02 06:00
MHDA- 2021/10/09 06:00
CRDT- 2021/10/01 06:10
PHST- 2021/04/23 00:00 [received]
PHST- 2021/07/10 00:00 [revised]
PHST- 2021/08/20 00:00 [accepted]
PHST- 2021/10/01 06:10 [entrez]
PHST- 2021/10/02 06:00 [pubmed]
PHST- 2021/10/09 06:00 [medline]
AID - 41/10/4761 [pii]
AID - 10.21873/anticanres.15291 [doi]
PST - ppublish
SO  - Anticancer Res. 2021 Oct;41(10):4761-4769. doi: 10.21873/anticanres.15291.