PMID- 34595321
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20220210
IS  - 2331-8325 (Electronic)
IS  - 2331-8325 (Linking)
VI  - 7
IP  - 24
DP  - 2017 Dec 20
TI  - Infection of Soybean Plants with the Insect Bacterial Symbiont Burkholderia 
      gladioli and Evaluation of Plant Fitness.
PG  - e2663
LID - 10.21769/BioProtoc.2663 [doi]
LID - e2663
AB  - To investigate the establishment and consequences of host-microbe interactions, 
      it is important to develop controlled infection assays suitable for each system, 
      as well as appropriate methods to evaluate successful infection and its 
      associated effects. Here, we describe a procedure for bacterial inoculation of 
      soybean plants, followed by the assessment of systemic infection and impact on 
      plant fitness. Soybean (Glycine max) seedlings were mechanically wounded using a 
      device that mimics insect herbivory and inoculated with known cell numbers of 
      Burkholderia gladioli bacteria previously isolated from an insect host. The 
      impact on the plants was evaluated by monitoring changes in height, time to 
      flowering and chlorophyll content during plant development, and by quantifying 
      seed production in comparison to plants inoculated with sterile water. The 
      presence and proliferation of bacterial infection were examined in tissues from 
      developed plants using quantitative PCR and fluorescence in situ hybridization 
      (FISH).
CI  - Copyright (c) 2017 The Authors; exclusive licensee Bio-protocol LLC.
FAU - Gaube, Paul
AU  - Gaube P
AD  - Insect Symbiosis Research Group, Max Planck Institute for Chemical Ecology, 
      Hans-Knoll-Strasse 8, Jena, Germany.
AD  - Molecular Biodiversity Group, Department of Animal Ecology and Tropical Biology 
      (Zoology III), Theodor Boveri-Institute, Julius Maximilian University, Am 
      Hubland, Wurzburg, Germany.
FAU - Kaltenpoth, Martin
AU  - Kaltenpoth M
AD  - Insect Symbiosis Research Group, Max Planck Institute for Chemical Ecology, 
      Hans-Knoll-Strasse 8, Jena, Germany.
AD  - Department for Evolutionary Ecology, Institute of Organismic and Molecular 
      Evolution, Johannes Gutenberg University, Johann-Joachim-Becher-Weg 13, Mainz, 
      Germany.
FAU - Florez, Laura V
AU  - Florez LV
AD  - Insect Symbiosis Research Group, Max Planck Institute for Chemical Ecology, 
      Hans-Knoll-Strasse 8, Jena, Germany.
AD  - Department for Evolutionary Ecology, Institute of Organismic and Molecular 
      Evolution, Johannes Gutenberg University, Johann-Joachim-Becher-Weg 13, Mainz, 
      Germany.
LA  - eng
PT  - Journal Article
DEP - 20171220
PL  - United States
TA  - Bio Protoc
JT  - Bio-protocol
JID - 101635102
PMC - PMC8438386
OTO - NOTNLM
OT  - Soybean (Glycine max)
OT  - Bacterial plant infection
OT  - Burkholderia gladioli
OT  - Microbe-host interactions
OT  - Plant fitness assay
OT  - Whole-mount FISH
EDAT- 2017/12/20 00:00
MHDA- 2017/12/20 00:01
PMCR- 2018/12/20
CRDT- 2021/10/01 07:37
PHST- 2017/10/10 00:00 [received]
PHST- 2017/11/24 00:00 [revised]
PHST- 2017/12/05 00:00 [accepted]
PHST- 2021/10/01 07:37 [entrez]
PHST- 2017/12/20 00:00 [pubmed]
PHST- 2017/12/20 00:01 [medline]
PHST- 2018/12/20 00:00 [pmc-release]
AID - e2663 [pii]
AID - 2663 [pii]
AID - 10.21769/BioProtoc.2663 [doi]
PST - epublish
SO  - Bio Protoc. 2017 Dec 20;7(24):e2663. doi: 10.21769/BioProtoc.2663. eCollection 
      2017 Dec 20.