PMID- 34597723
OWN - NLM
STAT- MEDLINE
DCOM- 20211222
LR  - 20211222
IS  - 1872-8006 (Electronic)
IS  - 0304-4165 (Linking)
VI  - 1865
IP  - 12
DP  - 2021 Dec
TI  - PACAP-derived mutant peptide MPAPO protects trigeminal ganglion cell and the 
      retina from hypoxic injury through anti-oxidative stress, anti-apoptosis, and 
      promoting axon regeneration.
PG  - 130018
LID - S0304-4165(21)00177-X [pii]
LID - 10.1016/j.bbagen.2021.130018 [doi]
AB  - The purpose of this study was to determine whether the MPAPO, derived peptide of 
      pituitary adenylate cyclase-activating polypeptide (PACAP), would protect 
      trigeminal ganglion cells (TGCs) and the mice retinas from a hypoxic insult. The 
      nerve endings of the ophthalmic nerve of the trigeminal nerve are widely 
      distributed in eye tissues. In TGCs after hypoxia exposure, we discovered that 
      reactive oxygen species level, the contents of cytosolic cytochrome c and 
      cleaved-caspase-3 were significantly increased, in the meanwhile, m-Calpain was 
      activated and cytoskeleton proteins (alphaII-spectrin and Synapsin) were degraded, 
      neurites of TGCs disappeared, but these effects were reversed in TGCs treated 
      with MPAPO. The structure of the mice retinas after hypoxic exposure was 
      disordered. Increased lipid peroxidation (LPO), decreased glutathione (GSH) 
      levels, and decreased superoxide dismutase (SOD) activity, positive cells of 
      terminal deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL), 
      the disintegration of nerve fibers was examined in the retinas following a 
      hypoxic insult. Disordered retina was attenuated with MPAPO eye drops, as well as 
      hypoxia-induced apoptosis in the developing retina, increase in LPO, and decrease 
      in GSH levels and SOD activity of the retina. Moreover, the disintegrated retinal 
      nerve fibers were reassembled after MPAPO treatment. These results suggest that 
      hypoxia induces oxidative stress, apoptosis, and neurites disruption, while MPAPO 
      is remarkably protective against these adverse effects of hypoxia in TGCs and the 
      developing retinas by specifically activating PAC1 receptor.
CI  - Copyright (c) 2021 Elsevier B.V. All rights reserved.
FAU - Li, Hui-Xian
AU  - Li HX
AD  - Institute of Biomedicine, Department of Cellular Biology, National Engineering 
      Research Center of Genetic Medicine, Key Laboratory of Bioengineering Medicine of 
      Guangdong Province, The national Demonstration center for Experimental Education 
      of Life Science and Technology, Jinan University, 601 Huangpu Ave West, 
      Guangzhou, 510632, Guangdong, People's Republic of China.
FAU - Feng, Jia
AU  - Feng J
AD  - Institute of Biomedicine, Department of Cellular Biology, National Engineering 
      Research Center of Genetic Medicine, Key Laboratory of Bioengineering Medicine of 
      Guangdong Province, The national Demonstration center for Experimental Education 
      of Life Science and Technology, Jinan University, 601 Huangpu Ave West, 
      Guangzhou, 510632, Guangdong, People's Republic of China.
FAU - Liu, Qian
AU  - Liu Q
AD  - Institute of Biomedicine, Department of Cellular Biology, National Engineering 
      Research Center of Genetic Medicine, Key Laboratory of Bioengineering Medicine of 
      Guangdong Province, The national Demonstration center for Experimental Education 
      of Life Science and Technology, Jinan University, 601 Huangpu Ave West, 
      Guangzhou, 510632, Guangdong, People's Republic of China.
FAU - Ou, Bi-Qian
AU  - Ou BQ
AD  - Institute of Biomedicine, Department of Cellular Biology, National Engineering 
      Research Center of Genetic Medicine, Key Laboratory of Bioengineering Medicine of 
      Guangdong Province, The national Demonstration center for Experimental Education 
      of Life Science and Technology, Jinan University, 601 Huangpu Ave West, 
      Guangzhou, 510632, Guangdong, People's Republic of China.
FAU - Lu, Shi-Yin
AU  - Lu SY
AD  - Institute of Biomedicine, Department of Cellular Biology, National Engineering 
      Research Center of Genetic Medicine, Key Laboratory of Bioengineering Medicine of 
      Guangdong Province, The national Demonstration center for Experimental Education 
      of Life Science and Technology, Jinan University, 601 Huangpu Ave West, 
      Guangzhou, 510632, Guangdong, People's Republic of China.
FAU - Ma, Yi
AU  - Ma Y
AD  - Institute of Biomedicine, Department of Cellular Biology, National Engineering 
      Research Center of Genetic Medicine, Key Laboratory of Bioengineering Medicine of 
      Guangdong Province, The national Demonstration center for Experimental Education 
      of Life Science and Technology, Jinan University, 601 Huangpu Ave West, 
      Guangzhou, 510632, Guangdong, People's Republic of China. Electronic address: 
      tmayi@jnu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210928
PL  - Netherlands
TA  - Biochim Biophys Acta Gen Subj
JT  - Biochimica et biophysica acta. General subjects
JID - 101731726
RN  - 0 (Pituitary Adenylate Cyclase-Activating Polypeptide)
SB  - IM
MH  - Axons
MH  - Nerve Regeneration
MH  - *Pituitary Adenylate Cyclase-Activating Polypeptide
OTO - NOTNLM
OT  - Apoptosis
OT  - Axon regeneration
OT  - Oxidative stress
OT  - PAC1 receptor
OT  - PACAP-derived mutant peptide (MPAPO)
OT  - Repair of hypoxia-injured trigeminal ganglion cells and the retinas
EDAT- 2021/10/02 06:00
MHDA- 2021/12/24 06:00
CRDT- 2021/10/01 20:15
PHST- 2021/01/07 00:00 [received]
PHST- 2021/08/30 00:00 [revised]
PHST- 2021/09/20 00:00 [accepted]
PHST- 2021/10/02 06:00 [pubmed]
PHST- 2021/12/24 06:00 [medline]
PHST- 2021/10/01 20:15 [entrez]
AID - S0304-4165(21)00177-X [pii]
AID - 10.1016/j.bbagen.2021.130018 [doi]
PST - ppublish
SO  - Biochim Biophys Acta Gen Subj. 2021 Dec;1865(12):130018. doi: 
      10.1016/j.bbagen.2021.130018. Epub 2021 Sep 28.