PMID- 34599242
OWN - NLM
STAT- MEDLINE
DCOM- 20211013
LR  - 20231102
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 11
IP  - 1
DP  - 2021 Oct 1
TI  - Viral detection and identification in 20 min by rapid single-particle 
      fluorescence in-situ hybridization of viral RNA.
PG  - 19579
LID - 10.1038/s41598-021-98972-z [doi]
LID - 19579
AB  - The increasing risk from viral outbreaks such as the ongoing COVID-19 pandemic 
      exacerbates the need for rapid, affordable and sensitive methods for virus 
      detection, identification and quantification; however, existing methods for 
      detecting virus particles in biological samples usually depend on multistep 
      protocols that take considerable time to yield a result. Here, we introduce a 
      rapid fluorescence in situ hybridization (FISH) protocol capable of detecting 
      influenza virus, avian infectious bronchitis virus and SARS-CoV-2 specifically 
      and quantitatively in approximately 20 min, in virus cultures, combined nasal and 
      throat swabs with added virus and likely patient samples without previous 
      purification. This fast and facile workflow can be adapted both as a lab 
      technique and a future diagnostic tool in enveloped viruses with an accessible 
      genome.
CI  - (c) 2021. The Author(s).
FAU - Hepp, Christof
AU  - Hepp C
AD  - Biological Physics Research Group, Clarendon Laboratory, Department of Physics, 
      University of Oxford, Oxford, OX1 3PU, UK. christof.hepp@physics.ox.ac.uk.
FAU - Shiaelis, Nicolas
AU  - Shiaelis N
AD  - Biological Physics Research Group, Clarendon Laboratory, Department of Physics, 
      University of Oxford, Oxford, OX1 3PU, UK.
FAU - Robb, Nicole C
AU  - Robb NC
AD  - Biological Physics Research Group, Clarendon Laboratory, Department of Physics, 
      University of Oxford, Oxford, OX1 3PU, UK.
AD  - Warwick Medical School, University of Warwick, Coventry, CV4 7AL, UK.
FAU - Vaughan, Alison
AU  - Vaughan A
AD  - Nuffield Department for Medicine, University of Oxford, Oxford, OX3 9DU, UK.
FAU - Matthews, Philippa C
AU  - Matthews PC
AD  - Nuffield Department for Medicine, University of Oxford, Oxford, OX3 9DU, UK.
FAU - Stoesser, Nicole
AU  - Stoesser N
AD  - Nuffield Department for Medicine, University of Oxford, Oxford, OX3 9DU, UK.
AD  - NIHR Health Protection Research Unit in Healthcare Associated Infections and 
      Antimicrobial Resistance at the University of Oxford in Partnership With Public 
      Health England, University of Oxford, Oxford, UK.
FAU - Crook, Derrick
AU  - Crook D
AD  - Nuffield Department for Medicine, University of Oxford, Oxford, OX3 9DU, UK.
AD  - NIHR Biomedical Research Centre, University of Oxford, Oxford, UK.
AD  - NIHR Health Protection Research Unit in Healthcare Associated Infections and 
      Antimicrobial Resistance at the University of Oxford in Partnership With Public 
      Health England, University of Oxford, Oxford, UK.
FAU - Kapanidis, Achillefs N
AU  - Kapanidis AN
AD  - Biological Physics Research Group, Clarendon Laboratory, Department of Physics, 
      University of Oxford, Oxford, OX1 3PU, UK. kapanidis@physics.ox.ac.uk.
LA  - eng
GR  - BB/V001868/1/BB_/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
GR  - 110164/Z/15/Z/WT_/Wellcome Trust/United Kingdom
GR  - MR/N010744/1/MRC_/Medical Research Council/United Kingdom
GR  - WT_/Wellcome Trust/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20211001
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (RNA, Viral)
SB  - IM
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - RNA, Viral/*isolation & purification
MH  - Viruses/genetics/*isolation & purification
PMC - PMC8486776
COIS- The work was carried out using a wide-field microscope from Oxford Nanoimaging, a 
      company in which A.N.K. is a co-founder and shareholder. The remaining authors 
      declare no competing interests.
EDAT- 2021/10/03 06:00
MHDA- 2021/10/14 06:00
PMCR- 2021/10/01
CRDT- 2021/10/02 06:02
PHST- 2021/05/12 00:00 [received]
PHST- 2021/09/09 00:00 [accepted]
PHST- 2021/10/02 06:02 [entrez]
PHST- 2021/10/03 06:00 [pubmed]
PHST- 2021/10/14 06:00 [medline]
PHST- 2021/10/01 00:00 [pmc-release]
AID - 10.1038/s41598-021-98972-z [pii]
AID - 98972 [pii]
AID - 10.1038/s41598-021-98972-z [doi]
PST - epublish
SO  - Sci Rep. 2021 Oct 1;11(1):19579. doi: 10.1038/s41598-021-98972-z.