PMID- 34600200
OWN - NLM
STAT- MEDLINE
DCOM- 20211216
LR  - 20211216
IS  - 1873-2542 (Electronic)
IS  - 0378-1135 (Linking)
VI  - 262
DP  - 2021 Nov
TI  - Disinfection and conditions associated with thermo-assisted drying and 
      decontamination inconsistently produce negative PRRSV rRT-PCR results on metal 
      surfaces.
PG  - 109240
LID - S0378-1135(21)00263-7 [pii]
LID - 10.1016/j.vetmic.2021.109240 [doi]
AB  - Because contaminated livestock trailers are a significant risk for transmitting 
      viruses between herds, various methods of washing, disinfecting, and 
      thermo-assisted drying and decontamination (TADD) have been evaluated for their 
      effectiveness in inactivating porcine reproductive and respiratory syndrome virus 
      (PRRSV) on contaminated surfaces. Information on when to expect negative qRT-PCR 
      results after adequate trailer sanitation is lacking. The objective of this study 
      was to evaluate whether there are conditions associated with 
      washing-disinfectant-TADD procedures that will consistently produce a negative 
      qRT-PCR result for the purpose of monitoring compliance with trailer sanitation 
      and decontamination protocols for PRRSV on metal surfaces. 144 diamond plate 
      aluminum coupons were spiked with PRRSV or phosphate-buffered saline (PBS) and 
      treated with a designated disinfectant protocol. Disinfectants evaluated included 
      multiple accelerated(R) hydrogen peroxide (AHP) disinfectants and a quaternary 
      ammonium and glutaraldehyde combination disinfectant. Disinfectant was applied 
      for 5 or 60 minutes of contact time at either 20  degrees C or -10  degrees C in a matrix of 
      feces or PBS. All coupons were heated until the surface temperature of the coupon 
      reached 71  degrees C and then held for 10 minutes to simulate TADD under field 
      conditions. Post-treatment swabs for all treatment groups, except negative 
      control groups, were positive by PRRSV qRT-PCR. Under the conditions evaluated in 
      this study, consistently negative qRT-PCR results after treatments were not 
      found. Therefore, for the purpose of monitoring compliance with trailer 
      sanitation and decontamination protocols for PRRSV, alternatives to qRT-PCR 
      should be explored.
CI  - Copyright (c) 2021 Elsevier B.V. All rights reserved.
FAU - Neat, Rita Anne
AU  - Neat RA
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, 1800 Christensen Drive, Ames, IA, 
      50011, United States. Electronic address: ritaneat@iastate.edu.
FAU - Jianqiang, Zhang
AU  - Jianqiang Z
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, 1800 Christensen Drive, Ames, IA, 
      50011, United States. Electronic address: jqzhang@iastate.edu.
FAU - Hoang, Hai
AU  - Hoang H
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, 1800 Christensen Drive, Ames, IA, 
      50011, United States. Electronic address: hai.hoangthanh@hcmuaf.edu.vn.
FAU - McKeen, Lauren
AU  - McKeen L
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, 1800 Christensen Drive, Ames, IA, 
      50011, United States. Electronic address: lamckeen@iastate.edu.
FAU - Mowrer, Christine L
AU  - Mowrer CL
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, 1800 Christensen Drive, Ames, IA, 
      50011, United States. Electronic address: clmowrer@iastate.edu.
FAU - Holtkamp, Derald J
AU  - Holtkamp DJ
AD  - Department of Veterinary Diagnostic and Production Animal Medicine, College of 
      Veterinary Medicine, Iowa State University, 1800 Christensen Drive, Ames, IA, 
      50011, United States. Electronic address: holtkamp@iastate.edu.
LA  - eng
PT  - Journal Article
DEP - 20210920
PL  - Netherlands
TA  - Vet Microbiol
JT  - Veterinary microbiology
JID - 7705469
RN  - 0 (Disinfectants)
RN  - 0 (Metals)
SB  - IM
MH  - Animals
MH  - *Decontamination/standards
MH  - Desiccation
MH  - Disinfectants/pharmacology
MH  - *Disinfection/standards
MH  - Hot Temperature
MH  - *Industrial Microbiology/methods/standards
MH  - *Metals
MH  - *Porcine respiratory and reproductive syndrome virus/genetics
MH  - *Reverse Transcriptase Polymerase Chain Reaction/veterinary
MH  - Swine
OTO - NOTNLM
OT  - PCR
OT  - PRRSV
OT  - Swine
OT  - biosecurity
EDAT- 2021/10/03 06:00
MHDA- 2021/12/17 06:00
CRDT- 2021/10/02 20:16
PHST- 2021/04/29 00:00 [received]
PHST- 2021/09/11 00:00 [accepted]
PHST- 2021/10/03 06:00 [pubmed]
PHST- 2021/12/17 06:00 [medline]
PHST- 2021/10/02 20:16 [entrez]
AID - S0378-1135(21)00263-7 [pii]
AID - 10.1016/j.vetmic.2021.109240 [doi]
PST - ppublish
SO  - Vet Microbiol. 2021 Nov;262:109240. doi: 10.1016/j.vetmic.2021.109240. Epub 2021 
      Sep 20.