PMID- 34600274
OWN - NLM
STAT- MEDLINE
DCOM- 20220304
LR  - 20220304
IS  - 1532-3102 (Electronic)
IS  - 0143-4004 (Linking)
VI  - 115
DP  - 2021 Nov
TI  - The effect of gestational diabetes on the expression of mitochondrial fusion 
      proteins in placental tissue.
PG  - 106-114
LID - S0143-4004(21)00602-0 [pii]
LID - 10.1016/j.placenta.2021.09.015 [doi]
AB  - INTRODUCTION: Gestational diabetes mellitus (GDM) poses a risk factor for fetal 
      mortality and morbidity by directly affecting the placenta and fetus. 
      Mitochondria are dynamic organelles that play a key role in energy production and 
      conversion in placental tissue. Mitochondrial fusion and fission proteins are 
      important in terms of providing mitochondrial dynamics, the adaptation of the 
      cell to different conditions, and maintaining the metabolic stability of the 
      cells. Although GDM shares many features with Type 2 diabetes mellitus (T2DM), 
      different effects of these conditions on the mother and the child suggest that 
      GDM may have specific pathological effects on placental cells. The aim of this 
      study is to investigate the expression of mitochondrial dynamics, and 
      mitochondrial protein folding markers in placentas from GDM patients and women 
      with pre-existing diabetes mellitus. METHODS: Placentas were properly collected 
      from women, who had pre-existing diabetes (Pre-DM), from women with gestational 
      diabetes mellitus (GDM) and from healthy (non-diabetic) pregnant women. Levels of 
      mitochondrial fusion markers were determined in these placentas by real time 
      quantitative PCR and Western blot experiments. RESULTS: mRNA expressions and 
      protein levels of mitochondrial fusion markers, mitofusin 1, mitofusin 2 (MFN1 
      and MFN2) and optical atrophy 1 (OPA1) proteins were found to be significantly 
      lower in both Pre-DM placentas and those with GDM compared to healthy 
      (non-diabetic) control group. Likewise, proteins involved in mitochondrial 
      protein folding were also found to be significantly reduced compared to control 
      group. DISCUSSION: Diabetes during pregnancy leads to processes that correlate 
      with mitochondria dysfunction in placenta. Our results showed that mitochondrial 
      fusion markers significantly decrease in placental tissue of women with GDM, 
      compared to the healthy non-diabetic women. The decrease in mitochondrial fusion 
      markers was more severe during GDM compared to the Pre-DM. Our results suggest 
      that there may be differences in the pathophysiology of these conditions.
CI  - Copyright (c) 2021 Elsevier Ltd. All rights reserved.
FAU - Kolac, Umut Kerem
AU  - Kolac UK
AD  - Department of Medical Biology, Faculty of Medicine, Aydin Adnan Menderes 
      University, AYDIN, Turkey. Electronic address: umutkolac@gmail.com.
FAU - Kurek Eken, Meryem
AU  - Kurek Eken M
AD  - Department of Reproductive Medicine and Infertility, Hisar Intercontinental 
      Hospital, ISTANBUL, Turkey. Electronic address: meryemkurekeken@gmail.com.
FAU - Unubol, Mustafa
AU  - Unubol M
AD  - Department of Endocrinology, Faculty of Medicine, Adnan Menderes University, 
      AYDIN, Turkey. Electronic address: drmunubol@yahoo.com.tr.
FAU - Donmez Yalcin, Gizem
AU  - Donmez Yalcin G
AD  - Department of Medical Biology, Faculty of Medicine, Aydin Adnan Menderes 
      University, AYDIN, Turkey. Electronic address: gizem.yalcin@adu.edu.tr.
FAU - Yalcin, Abdullah
AU  - Yalcin A
AD  - Department of Medical Biology, Faculty of Medicine, Aydin Adnan Menderes 
      University, AYDIN, Turkey. Electronic address: abdullah.yalcin@adu.edu.tr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20210924
PL  - Netherlands
TA  - Placenta
JT  - Placenta
JID - 8006349
RN  - 0 (Mitochondrial Membrane Transport Proteins)
RN  - 0 (Mitochondrial Proteins)
RN  - 0 (RNA, Messenger)
RN  - EC 3.6.1.- (GTP Phosphohydrolases)
RN  - EC 3.6.1.- (MFN2 protein, human)
RN  - EC 3.6.1.- (OPA1 protein, human)
RN  - EC 3.6.5.- (Mfn1 protein, human)
SB  - IM
MH  - Adult
MH  - Body Mass Index
MH  - Diabetes, Gestational/*metabolism
MH  - Female
MH  - GTP Phosphohydrolases/genetics
MH  - Gene Expression/*physiology
MH  - Humans
MH  - Mitochondrial Dynamics/*physiology
MH  - Mitochondrial Membrane Transport Proteins/genetics
MH  - Mitochondrial Proteins/*genetics
MH  - Obesity/complications/metabolism
MH  - Placenta/*metabolism
MH  - Pregnancy
MH  - Pregnancy Complications/metabolism
MH  - RNA, Messenger/analysis
OTO - NOTNLM
OT  - Gestational diabetes mellitus
OT  - Mitochondrial fusion
OT  - Placenta
EDAT- 2021/10/03 06:00
MHDA- 2022/03/05 06:00
CRDT- 2021/10/02 20:19
PHST- 2020/10/25 00:00 [received]
PHST- 2021/09/08 00:00 [revised]
PHST- 2021/09/21 00:00 [accepted]
PHST- 2021/10/03 06:00 [pubmed]
PHST- 2022/03/05 06:00 [medline]
PHST- 2021/10/02 20:19 [entrez]
AID - S0143-4004(21)00602-0 [pii]
AID - 10.1016/j.placenta.2021.09.015 [doi]
PST - ppublish
SO  - Placenta. 2021 Nov;115:106-114. doi: 10.1016/j.placenta.2021.09.015. Epub 2021 
      Sep 24.