PMID- 34600998
OWN - NLM
STAT- MEDLINE
DCOM- 20211203
LR  - 20211214
IS  - 1879-1026 (Electronic)
IS  - 0048-9697 (Linking)
VI  - 806
IP  - Pt 2
DP  - 2022 Feb 1
TI  - When FLOW-FISH met FACS: Combining multiparametric, dynamic approaches for 
      microbial single-cell research in the total environment.
PG  - 150682
LID - S0048-9697(21)05760-0 [pii]
LID - 10.1016/j.scitotenv.2021.150682 [doi]
AB  - In environmental microbiology, the ability to assess, in a high-throughput way, 
      single-cells within microbial communities is key to understand their 
      heterogeneity. Fluorescence in situ hybridization (FISH) uses fluorescently 
      labeled oligonucleotide probes to detect, identify, and quantify single cells of 
      specific taxonomic groups. The combination of Flow Cytometry (FLOW) with FISH 
      (FLOW-FISH) enables high-throughput quantification of complex whole cell 
      populations, which when associated with fluorescence-activated cell sorting 
      (FACS) enables sorting of target microorganisms. These sorted cells may be 
      investigated in many ways, for instance opening new avenues for cytomics at a 
      single-cell scale. In this review, an overview of FISH and FLOW methodologies is 
      provided, addressing conventional methods, signal amplification approaches, 
      common fluorophores for cell physiology parameters evaluation, and model 
      variation techniques as well. The coupling of FLOW-FISH-FACS is explored in the 
      context of different downstream applications of sorted cells. Current and 
      emerging applications in environmental microbiology to outline the interactions 
      and processes of complex microbial communities within soil, water, animal 
      microbiota, polymicrobial biofilms, and food samples, are described.
CI  - Copyright (c) 2021 Elsevier B.V. All rights reserved.
FAU - Pereira, Andre C
AU  - Pereira AC
AD  - Centre for Ecology, Evolution and Environmental Changes (cE3c), Faculdade de 
      Ciencias da Universidade de Lisboa, Lisboa, Portugal; Biosystems & Integrative 
      Sciences Institute (BioISI), Faculdade de Ciencias da Universidade de Lisboa, 
      Lisboa, Portugal.
FAU - Tenreiro, Ana
AU  - Tenreiro A
AD  - Biosystems & Integrative Sciences Institute (BioISI), Faculdade de Ciencias da 
      Universidade de Lisboa, Lisboa, Portugal.
FAU - Cunha, Monica V
AU  - Cunha MV
AD  - Centre for Ecology, Evolution and Environmental Changes (cE3c), Faculdade de 
      Ciencias da Universidade de Lisboa, Lisboa, Portugal; Biosystems & Integrative 
      Sciences Institute (BioISI), Faculdade de Ciencias da Universidade de Lisboa, 
      Lisboa, Portugal. Electronic address: mscunha@fc.ul.pt.
LA  - eng
PT  - Journal Article
PT  - Review
DEP - 20210930
PL  - Netherlands
TA  - Sci Total Environ
JT  - The Science of the total environment
JID - 0330500
SB  - IM
MH  - Animals
MH  - Environmental Microbiology
MH  - Flow Cytometry
MH  - In Situ Hybridization, Fluorescence
MH  - *Microbiota
OTO - NOTNLM
OT  - Environmental microbiology
OT  - FACS
OT  - FISH
OT  - Flow cytometry
OT  - Single-cell
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2021/10/04 06:00
MHDA- 2021/12/15 06:00
CRDT- 2021/10/03 20:39
PHST- 2021/08/14 00:00 [received]
PHST- 2021/09/22 00:00 [revised]
PHST- 2021/09/26 00:00 [accepted]
PHST- 2021/10/04 06:00 [pubmed]
PHST- 2021/12/15 06:00 [medline]
PHST- 2021/10/03 20:39 [entrez]
AID - S0048-9697(21)05760-0 [pii]
AID - 10.1016/j.scitotenv.2021.150682 [doi]
PST - ppublish
SO  - Sci Total Environ. 2022 Feb 1;806(Pt 2):150682. doi: 
      10.1016/j.scitotenv.2021.150682. Epub 2021 Sep 30.