PMID- 34601015
OWN - NLM
STAT- MEDLINE
DCOM- 20211229
LR  - 20211229
IS  - 1879-260X (Electronic)
IS  - 0925-4439 (Linking)
VI  - 1868
IP  - 1
DP  - 2022 Jan 1
TI  - Comparison of bovine serum albumin glycation by ribose and fructose in vitro and 
      in vivo.
PG  - 166283
LID - S0925-4439(21)00216-7 [pii]
LID - 10.1016/j.bbadis.2021.166283 [doi]
AB  - Advanced glycation end products (AGEs) play a critical pathogenic role in the 
      development of diabetic complications. Recent studies have shown that diabetes is 
      associated with not only abnormal glucose metabolism but also abnormal ribose and 
      fructose metabolism, although glucose is present at the highest concentration in 
      humans. The glycation ability and contribution of ribose and fructose to diabetic 
      complications remain unclear. Here, the glycation ability of ribose, fructose and 
      glucose under a mimic physiological condition, in which the concentration of 
      ribose or fructose was one-fiftieth that of glucose, was compared. Bovine serum 
      albumin (BSA) was used as the working protein in our experiments. Ribose 
      generated more AGEs and was markedly more cytotoxic to SH-SY5Y cells than 
      fructose. The first-order rate constant of ribose glycation was found to be 
      significantly greater than that of fructose glycation. LC-MS/MS analysis revealed 
      41 ribose-glycated Lys residues and 12 fructose-glycated residues. Except for the 
      shared Lys residues, ribose reacted selectively with 17 Lys, while no selective 
      Lys was found in fructose-glycated BSA. Protein conformational changes suggested 
      that ribose glycation may induce BSA into amyloid-like monomers compared with 
      fructose glycation. The levels of serum ribose were correlated positively with 
      glycated serum protein (GSP) and diabetic duration in type 2 diabetes mellitus 
      (T2DM), respectively. These results indicate that ribose has a greater glycation 
      ability than fructose, while ribose largely contributes to the production of AGEs 
      and provides a new insight to understand in the occurrence and development of 
      diabetes complications.
CI  - Copyright (c) 2021 Elsevier B.V. All rights reserved.
FAU - Mou, Lixian
AU  - Mou L
AD  - Basic College of Medicine, Southwest Medical University, Luzhou, Sichuan 646000, 
      China; State Key Laboratory of Brain and Cognitive Science, Institute of 
      Biophysics, University of Chinese Academy of Sciences, Beijing 100101, China; 
      Department of Nuclear Medicine, Affiliated Hospital of Southwest Medical 
      University, No. 25, Taiping St., Luzhou, Sichuan 646000, China; Nuclear Medicine 
      and Molecular Imaging Key Laboratory of Sichuan Province, Luzhou, Sichuan 646000, 
      China; Academician (Expert) Workstation of Sichuan Province, 646000, China.
FAU - Hu, Pingdong
AU  - Hu P
AD  - State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, 
      University of Chinese Academy of Sciences, Beijing 100101, China.
FAU - Cao, Xiao
AU  - Cao X
AD  - Basic College of Medicine, Southwest Medical University, Luzhou, Sichuan 646000, 
      China; State Key Laboratory of Brain and Cognitive Science, Institute of 
      Biophysics, University of Chinese Academy of Sciences, Beijing 100101, China.
FAU - Chen, Yue
AU  - Chen Y
AD  - Department of Nuclear Medicine, Affiliated Hospital of Southwest Medical 
      University, No. 25, Taiping St., Luzhou, Sichuan 646000, China; Nuclear Medicine 
      and Molecular Imaging Key Laboratory of Sichuan Province, Luzhou, Sichuan 646000, 
      China; Academician (Expert) Workstation of Sichuan Province, 646000, China.
FAU - Xu, Yong
AU  - Xu Y
AD  - Basic College of Medicine, Southwest Medical University, Luzhou, Sichuan 646000, 
      China.
FAU - He, Tao
AU  - He T
AD  - Basic College of Medicine, Southwest Medical University, Luzhou, Sichuan 646000, 
      China.
FAU - Wei, Yan
AU  - Wei Y
AD  - State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, 
      University of Chinese Academy of Sciences, Beijing 100101, China. Electronic 
      address: yanwei@ibp.ac.cn.
FAU - He, Rongqiao
AU  - He R
AD  - Basic College of Medicine, Southwest Medical University, Luzhou, Sichuan 646000, 
      China; State Key Laboratory of Brain and Cognitive Science, Institute of 
      Biophysics, University of Chinese Academy of Sciences, Beijing 100101, China; CAS 
      Key Laboratory of Mental Health, Institute of Psychology, University of Chinese 
      Academy of Sciences, Beijing 100101, China. Electronic address: herq@ibp.ac.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20211001
PL  - Netherlands
TA  - Biochim Biophys Acta Mol Basis Dis
JT  - Biochimica et biophysica acta. Molecular basis of disease
JID - 101731730
RN  - 0 (Glycation End Products, Advanced)
RN  - 27432CM55Q (Serum Albumin, Bovine)
RN  - 30237-26-4 (Fructose)
RN  - 681HV46001 (Ribose)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - Chromatography, Liquid
MH  - Diabetes Complications/*blood/metabolism/pathology
MH  - Diabetes Mellitus, Type 2/*blood/genetics/pathology
MH  - Fructose/blood
MH  - Glucose/metabolism
MH  - Glycation End Products, Advanced/*genetics/metabolism
MH  - Glycosylation
MH  - Humans
MH  - Ribose/blood
MH  - Serum Albumin, Bovine/*metabolism
MH  - Tandem Mass Spectrometry
OTO - NOTNLM
OT  - Cytotoxic
OT  - Fructose
OT  - Glycation
OT  - Monomer
OT  - Ribose
OT  - Type 2 diabetes mellitus
EDAT- 2021/10/04 06:00
MHDA- 2021/12/30 06:00
CRDT- 2021/10/03 20:40
PHST- 2021/05/18 00:00 [received]
PHST- 2021/08/24 00:00 [revised]
PHST- 2021/09/22 00:00 [accepted]
PHST- 2021/10/04 06:00 [pubmed]
PHST- 2021/12/30 06:00 [medline]
PHST- 2021/10/03 20:40 [entrez]
AID - S0925-4439(21)00216-7 [pii]
AID - 10.1016/j.bbadis.2021.166283 [doi]
PST - ppublish
SO  - Biochim Biophys Acta Mol Basis Dis. 2022 Jan 1;1868(1):166283. doi: 
      10.1016/j.bbadis.2021.166283. Epub 2021 Oct 1.