PMID- 34602123
OWN - NLM
STAT- MEDLINE
DCOM- 20220209
LR  - 20220209
IS  - 1448-5990 (Electronic)
IS  - 1031-3613 (Linking)
VI  - 33
IP  - 12
DP  - 2021 Oct
TI  - Sperm storage in the oviduct of the Chinese pond turtle Mauremys reevesii depends 
      on oestrogen-based suppression of the TLR2/4 immune pathway.
PG  - 736-745
LID - 10.1071/RD20341 [doi]
AB  - The long-term storage of spermatozoa in the female reproductive tract is limited 
      by the innate immune system. Oestrogen plays a role in regulating the innate 
      immune system. Thus, exploring the expression of genes in the Toll-like receptor 
      (TLR) 2/4 pathway and oestrogen receptors in the oviduct of Mauremys reevesii 
      could contribute to our understanding of the mechanism of sperm storage. In this 
      study, three parts of the oviduct (isthmus, uterus and vagina) in three mated and 
      unmated female turtles were used to perform immunohistochemistry and real-time 
      quantitative polymerase chain reaction (qPCR). Immunohistochemistry revealed that 
      the TLR2/4 protein was mainly distributed in epithelial tissues and glandular 
      cell membranes, and that TLR2/4 levels in the oviduct were significantly 
      decreased in mated compared with unmated turtles. Real-time qPCR indicated that 
      TLR2/4, myeloid differentiation factor 88 (MyD88), interleukin 1 receptor 
      associated kinase 4 (IRAK4), TNF receptor associated factor 6 (TRAF6), interferon 
      regulatory factor 3 (IRF3) and interleukin 6 (IL6) mRNA expression was 
      significantly higher in the oviduct of unmated than mated turtles, whereas the 
      opposite was true for the expression of oestrogen receptor 1 (ESR1) and 
      progesterone receptor (PGR). These results indicate that when spermatozoa are 
      stored in the oviduct, an increase in oestrogen suppresses the immune response 
      induced by the TLR2/4 pathway so that spermatozoa are not removed as a foreign 
      substance, but stored until fertilisation. The findings of this study are 
      relevant to our understanding of the relationship between sperm storage and the 
      innate immune system in the oviduct of reptiles.
FAU - Cai, Wenlong
AU  - Cai W
AD  - Anhui Provincial Key Laboratory of the Conservation and Exploitation of 
      Biological Resources, College of Life Sciences, Anhui Normal University, Wuhu 
      241000, Anhui, China.
FAU - Chen, Wei
AU  - Chen W
AD  - Anhui Provincial Key Laboratory of the Conservation and Exploitation of 
      Biological Resources, College of Life Sciences, Anhui Normal University, Wuhu 
      241000, Anhui, China.
FAU - Wang, Yajie
AU  - Wang Y
AD  - Anhui Provincial Key Laboratory of the Conservation and Exploitation of 
      Biological Resources, College of Life Sciences, Anhui Normal University, Wuhu 
      241000, Anhui, China.
FAU - Bu, Xingjiang
AU  - Bu X
AD  - Anhui Provincial Key Laboratory of the Conservation and Exploitation of 
      Biological Resources, College of Life Sciences, Anhui Normal University, Wuhu 
      241000, Anhui, China; and Corresponding author. Email: buxingjiang@163.com.
FAU - Xia, Xingquan
AU  - Xia X
AD  - Anhui Provincial Key Laboratory of the Conservation and Exploitation of 
      Biological Resources, College of Life Sciences, Anhui Normal University, Wuhu 
      241000, Anhui, China.
FAU - Nie, Liuwang
AU  - Nie L
AD  - Anhui Provincial Key Laboratory of the Conservation and Exploitation of 
      Biological Resources, College of Life Sciences, Anhui Normal University, Wuhu 
      241000, Anhui, China.
LA  - eng
PT  - Journal Article
PL  - Australia
TA  - Reprod Fertil Dev
JT  - Reproduction, fertility, and development
JID - 8907465
RN  - 0 (Receptors, Estrogen)
RN  - 0 (Toll-Like Receptor 2)
RN  - 0 (Toll-Like Receptor 4)
SB  - IM
MH  - Animals
MH  - Female
MH  - Immunity, Innate/*physiology
MH  - Male
MH  - Oviducts/*metabolism
MH  - Receptors, Estrogen/*physiology
MH  - Spermatozoa/*physiology
MH  - Toll-Like Receptor 2/*metabolism
MH  - Toll-Like Receptor 4/*metabolism
MH  - Turtles
EDAT- 2021/10/05 06:00
MHDA- 2022/02/10 06:00
CRDT- 2021/10/04 05:32
PHST- 2020/12/28 00:00 [received]
PHST- 2021/07/08 00:00 [accepted]
PHST- 2021/10/05 06:00 [pubmed]
PHST- 2022/02/10 06:00 [medline]
PHST- 2021/10/04 05:32 [entrez]
AID - RD20341 [pii]
AID - 10.1071/RD20341 [doi]
PST - ppublish
SO  - Reprod Fertil Dev. 2021 Oct;33(12):736-745. doi: 10.1071/RD20341.