PMID- 34603753
OWN - NLM
STAT- MEDLINE
DCOM- 20220329
LR  - 20220427
IS  - 2309-995X (Electronic)
IS  - 2076-4243 (Print)
IS  - 2076-4243 (Linking)
VI  - 13
IP  - 3
DP  - 2021
TI  - Asynchronous DNA Replication of Biallelically Expressed Genes in Human Peripheral 
      Blood Lymphocytes as a Prognostic Sign of Cancer.
PG  - 33-38
LID - 10.17691/stm2021.13.3.04 [doi]
AB  - The aim of the study was to identify and quantify lymphocytes with asynchronous 
      replication of the AURKA and TP53 genes in cancer patients versus controls and to 
      assess the diagnostic capabilities of this approach. MATERIALS AND METHODS: The 
      study was carried out with peripheral blood lymphocytes probed for the AURKA and 
      TP53 genes using the interphase fluorescence in situ hybridization (FISH) method 
      (Vysis, USA and Kreatech, The Netherlands). The control group included 70 people: 
      clinically healthy donors and patients with non-oncological diseases such as 
      gastritis, pancreatitis, chronic calculous cholecystitis, bronchial asthma, 
      peptic ulcer disease, inguinal hernia, arthrosis, myoma, hepatitis, epilepsy, 
      chronic prostatitis, chronic tonsillitis, and rectal adenoma. The group of cancer 
      patients included 219 people with various oncological diseases: gastric cancer 
      (n=68), colorectal cancer (n=30), chronic lymphocytic leukemia (n=52), Hodgkin 
      lymphoma (n=33), and polyneoplasia (n=41). RESULTS: In the control group, the 
      mean frequency of lymphocytes with asynchronous gene replication (AGR) was 
      22.0+/-3.4% for AURKA and 18.0+/-3.2% for TP53; in the group of cancer patients, that 
      was 36.8+/-4.8 and 28.4+/-5.1%, respectively. The excessive presence of lymphocytes 
      with the AGR in cancer patients was consistent and statistically significant 
      (p<0.0001). For the AURKA gene, the AGR-based cancer detection showed a 
      sensitivity of 98.6+/-0.7%, a specificity of 100%, and an accuracy of 98.3+/-0.8%, 
      and for the TP53 gene - 78.6+/-3.1, 98.5+/-0.9, and 85.9+/-2.6%, respectively. 
      CONCLUSION: This pilot study on lymphocytes with AGR of AURKA and TP53 genes in 
      cancer patients can serve a basis for creating a new molecular cytogenetic 
      technology for detecting malignant neoplasms in humans.
FAU - Tsepenko, V V
AU  - Tsepenko VV
AD  - Senior Researcher, Laboratory of Molecular and Genetic Pathology, Department of 
      Clinical Morphology; A. Tsyb Medical Radiological Research Centre - Branch of the 
      National Medical Research Radiological Centre of the Ministry of Health of the 
      Russian Federation, 4 Korolev St., Obninsk, 249036, Russia.
FAU - Shkavrova, T G
AU  - Shkavrova TG
AD  - Senior Researcher, Laboratory of Molecular and Genetic Pathology, Department of 
      Clinical Morphology; A. Tsyb Medical Radiological Research Centre - Branch of the 
      National Medical Research Radiological Centre of the Ministry of Health of the 
      Russian Federation, 4 Korolev St., Obninsk, 249036, Russia.
FAU - Cherkesov, V N
AU  - Cherkesov VN
AD  - Head of the Laboratory for Quality Control of Medical Care; A. Tsyb Medical 
      Radiological Research Centre - Branch of the National Medical Research 
      Radiological Centre of the Ministry of Health of the Russian Federation, 4 
      Korolev St., Obninsk, 249036, Russia.
FAU - Golub, E V
AU  - Golub EV
AD  - Leading Researcher, Laboratory of Molecular and Genetic Pathology, Department of 
      Clinical Morphology; A. Tsyb Medical Radiological Research Centre - Branch of the 
      National Medical Research Radiological Centre of the Ministry of Health of the 
      Russian Federation, 4 Korolev St., Obninsk, 249036, Russia.
FAU - Mikhailova, G F
AU  - Mikhailova GF
AD  - Head of the Laboratory of Molecular and Genetic Pathology, Department of Clinical 
      Morphology; A. Tsyb Medical Radiological Research Centre - Branch of the National 
      Medical Research Radiological Centre of the Ministry of Health of the Russian 
      Federation, 4 Korolev St., Obninsk, 249036, Russia.
LA  - eng
PT  - Journal Article
DEP - 20210628
PL  - Russia (Federation)
TA  - Sovrem Tekhnologii Med
JT  - Sovremennye tekhnologii v meditsine
JID - 101604515
SB  - IM
MH  - DNA Replication
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *Leukemia, Lymphocytic, Chronic, B-Cell/genetics
MH  - Lymphocytes/pathology
MH  - Male
MH  - Pilot Projects
MH  - Prognosis
PMC - PMC8482818
OTO - NOTNLM
OT  - *AURKA
OT  - *FISH
OT  - *TP53
OT  - *asynchronous DNA replication
OT  - *diagnosis of malignant neoplasms
OT  - *fluorescence in situ hybridization
OT  - *peripheral blood lymphocytes
COIS- Conflicts of interest. The authors have no conflicts of interest related to this 
      publication.
EDAT- 2021/10/05 06:00
MHDA- 2022/03/30 06:00
PMCR- 2021/01/01
CRDT- 2021/10/04 06:03
PHST- 2020/08/25 00:00 [received]
PHST- 2021/10/04 06:03 [entrez]
PHST- 2021/10/05 06:00 [pubmed]
PHST- 2022/03/30 06:00 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 10.17691/stm2021.13.3.04 [doi]
PST - ppublish
SO  - Sovrem Tekhnologii Med. 2021;13(3):33-38. doi: 10.17691/stm2021.13.3.04. Epub 
      2021 Jun 28.