PMID- 34606539
OWN - NLM
STAT- MEDLINE
DCOM- 20220117
LR  - 20220117
IS  - 2042-650X (Electronic)
IS  - 2042-6496 (Linking)
VI  - 12
IP  - 19
DP  - 2021 Oct 4
TI  - Effect of Lactiplantibacillus plantarum HM-22 on immunoregulation and intestinal 
      microbiota in alpha-lactalbumin-induced allergic mice.
PG  - 8887-8898
LID - 10.1039/d1fo01703a [doi]
AB  - Milk protein is one of the eight major allergens, and alpha-lactalbumin (alpha-LA) is one 
      of the major allergens of bovine milk protein. Our previous studies found that 
      Lactiplantibacillus plantarum HM-22 (L. plantarum HM-22) showed a good 
      gastrointestinal survival rate and intestinal colonization. To investigate the 
      effect of L. plantarum HM-22 on intestinal inflammation and intestinal microbiota 
      in alpha-LA-induced allergic mice, in this study, L. plantarum HM-22 at low and high 
      doses was intragastrically administered to alpha-LA-induced allergic mice for 5 
      weeks. The results showed that L. plantarum HM-22 significantly relieved the 
      weight loss and organ index of alpha-LA-induced allergic mice (p < 0.05). L. 
      plantarum HM-22 increased the levels of interleukin-10 (IL-10), interferon-gamma 
      (IFN-gamma) and transforming growth factor-beta (TGF-beta) in the serum of alpha-LA-induced 
      allergic mice and decreased the levels of total immunoglobulin E (IgE) and the 
      proinflammatory factor interleukin-4 (IL-4) (p < 0.05). The crypt structure of 
      the colon tissues of alpha-LA-induced allergic mice changed, goblet cells decreased, 
      and the phenomenon of a large number of inflammatory corpuscles that appeared was 
      improved and alleviated with the intervention of L. plantarum HM-22 by 
      hematoxylin-eosin (HE) staining. Western blot analysis showed that L. plantarum 
      HM-22 significantly increased the expression of occludin and claudin-1 in the 
      colon of alpha-LA-induced allergic mice and decreased the expression of the 
      inflammatory proteins p65 and IkappaBalpha (p < 0.05). The intestinal microbiota of mice 
      in each group was determined by 16S rRNA amplicon sequencing, and the results 
      showed that intervention with L. plantarum HM-22 improved the intestinal microbes 
      of alpha-LA-induced allergic mice. Spearman's correlation analysis revealed the 
      correlation between intestinal microbiota changes and the alpha-LA-induced 
      allergy-related index. This study provides a theoretical basis for probiotics to 
      prevent allergies by changing the intestinal microbiota.
FAU - Jiang, Shujuan
AU  - Jiang S
AUID- ORCID: 0000-0002-4527-0747
AD  - School of Food Science and Technology, Dalian Polytechnic University, Dalian, 
      116034, China. gq6552002@aliyun.com.
FAU - Hou, Yaqi
AU  - Hou Y
AD  - School of Food Science and Technology, Dalian Polytechnic University, Dalian, 
      116034, China. gq6552002@aliyun.com.
FAU - Meng, Lingying
AU  - Meng L
AD  - School of Food Science and Technology, Dalian Polytechnic University, Dalian, 
      116034, China. gq6552002@aliyun.com.
FAU - Pu, Xueli
AU  - Pu X
AD  - School of Food Science and Technology, Dalian Polytechnic University, Dalian, 
      116034, China. gq6552002@aliyun.com.
FAU - Zhu, Xuemei
AU  - Zhu X
AD  - School of Food Science and Technology, Dalian Polytechnic University, Dalian, 
      116034, China. gq6552002@aliyun.com.
FAU - Tuo, Yanfeng
AU  - Tuo Y
AUID- ORCID: 0000-0003-2874-4354
AD  - School of Food Science and Technology, Dalian Polytechnic University, Dalian, 
      116034, China. gq6552002@aliyun.com.
FAU - Qian, Fang
AU  - Qian F
AD  - School of Food Science and Technology, Dalian Polytechnic University, Dalian, 
      116034, China. gq6552002@aliyun.com.
FAU - Mu, Guangqing
AU  - Mu G
AUID- ORCID: 0000-0001-9356-753X
AD  - School of Food Science and Technology, Dalian Polytechnic University, Dalian, 
      116034, China. gq6552002@aliyun.com.
LA  - eng
PT  - Journal Article
DEP - 20211004
PL  - England
TA  - Food Funct
JT  - Food & function
JID - 101549033
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Animals
MH  - Disease Models, Animal
MH  - Female
MH  - Gastrointestinal Microbiome/drug effects
MH  - Immunoglobulin E/blood/drug effects
MH  - *Lactobacillus plantarum
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - Milk Hypersensitivity/*prevention & control
MH  - Probiotics/*administration & dosage/pharmacology
EDAT- 2021/10/05 06:00
MHDA- 2022/01/18 06:00
CRDT- 2021/10/04 17:29
PHST- 2021/10/04 17:29 [entrez]
PHST- 2021/10/05 06:00 [pubmed]
PHST- 2022/01/18 06:00 [medline]
AID - 10.1039/d1fo01703a [doi]
PST - epublish
SO  - Food Funct. 2021 Oct 4;12(19):8887-8898. doi: 10.1039/d1fo01703a.