PMID- 34607472
OWN - NLM
STAT- MEDLINE
DCOM- 20220622
LR  - 20220622
IS  - 1473-7760 (Electronic)
IS  - 1052-0295 (Linking)
VI  - 97
IP  - 5
DP  - 2022 Jul
TI  - Mesenchymal stem cells seeded onto nanofiber scaffold for myocardial 
      regeneration.
PG  - 322-333
LID - 10.1080/10520295.2021.1979251 [doi]
AB  - Cardiac disease is the leading cause of mortality and disability worldwide. We 
      investigated the role of undifferentiated adipose tissue-derived mesenchymal stem 
      cells (ADMSC) alone and ADMSC seeded onto the electro-spun nanofibers (NF) for 
      reconstructing damaged cardiac tissue in isoprenaline-induced myocardial 
      infarction (MI) in rats. ADMSC were sorted by morphological appearance and by 
      detection of cluster of differentiation (CD) surface antigens. The therapeutic 
      potential of ADMSC for treating MI was evaluated by electrocardiogram (ECG), 
      biochemical analysis, molecular genetic analysis and histological examination. 
      Treatment of MI-challenged rats with ADMSC improved ECG findings, which were 
      corroborated by significant decreases in serum lactate dehydrogenase (LDH) and 
      creatine kinase-MB (CK-MB) enzyme activities together with reduced serum troponin 
      T (cTnT) and connexin 43 (Cx43) levels. MI model rats treated with ADMSC 
      exhibited a significant increase in serum alpha sarcomeric actin (Actn) and GATA 
      binding protein 4 (GATA4), and NK2 homeobox 5 (NKX2.5) gene expression was 
      decreased following treatment with ADMSC. ADMSC also ameliorated damage to 
      cardiac tissue. The effects of ADMSC seeded onto NF were superior to those of 
      ADMSC alone. ADMSC may be useful for mitigation of MI.
FAU - Kishta, Mohamed S
AU  - Kishta MS
AD  - Hormones Department, Medical Research Division, National Research Centre, Giza, 
      Egypt.
AD  - Stem Cell Lab, Center of Excellence for Advanced Sciences, National Research 
      Centre, Giza, Egypt.
FAU - Ahmed, Hanaa H
AU  - Ahmed HH
AD  - Hormones Department, Medical Research Division, National Research Centre, Giza, 
      Egypt.
AD  - Stem Cell Lab, Center of Excellence for Advanced Sciences, National Research 
      Centre, Giza, Egypt.
FAU - Ali, Mohamed A M
AU  - Ali MAM
AD  - Biochemistry Department, Faculty of Science, Ain Shams University, Cairo, Egypt.
FAU - Aglan, Hadeer A
AU  - Aglan HA
AD  - Hormones Department, Medical Research Division, National Research Centre, Giza, 
      Egypt.
AD  - Stem Cell Lab, Center of Excellence for Advanced Sciences, National Research 
      Centre, Giza, Egypt.
FAU - Mohamed, Mohamed Ragaa
AU  - Mohamed MR
AD  - Biochemistry Department, Faculty of Science, Ain Shams University, Cairo, Egypt.
LA  - eng
PT  - Journal Article
DEP - 20211005
PL  - England
TA  - Biotech Histochem
JT  - Biotechnic & histochemistry : official publication of the Biological Stain 
      Commission
JID - 9107378
SB  - IM
MH  - Adipose Tissue
MH  - Animals
MH  - *Mesenchymal Stem Cells
MH  - *Myocardial Infarction/therapy
MH  - *Nanofibers
MH  - Rats
MH  - Regeneration
OTO - NOTNLM
OT  - Adipose tissue
OT  - biomarkers
OT  - electro-spun
OT  - isoprenaline
OT  - mesenchymal stem cells
OT  - myocardial infarction
OT  - nanofibers
OT  - rats
EDAT- 2021/10/06 06:00
MHDA- 2022/06/23 06:00
CRDT- 2021/10/05 05:29
PHST- 2021/10/06 06:00 [pubmed]
PHST- 2022/06/23 06:00 [medline]
PHST- 2021/10/05 05:29 [entrez]
AID - 10.1080/10520295.2021.1979251 [doi]
PST - ppublish
SO  - Biotech Histochem. 2022 Jul;97(5):322-333. doi: 10.1080/10520295.2021.1979251. 
      Epub 2021 Oct 5.