PMID- 34607665
OWN - NLM
STAT- MEDLINE
DCOM- 20211006
LR  - 20211006
IS  - 1001-0742 (Print)
IS  - 1001-0742 (Linking)
VI  - 109
DP  - 2021 Nov
TI  - DNA methylation changes induced by BDE-209 are related to DNA damage response and 
      germ cell development in GC-2spd.
PG  - 161-170
LID - S1001-0742(21)00134-0 [pii]
LID - 10.1016/j.jes.2021.04.001 [doi]
AB  - Decabrominated diphenyl ether (BDE-209) is generally utilized in multiple polymer 
      materials as common brominated flame retardant. BDE-209 has been listed as 
      persistent organic pollutants (POPs), which was considered to be reproductive 
      toxin in the environment. But it still remains unclear about the effects of 
      BDE-209 on DNA methylation and the induced-male reproductive toxicity. Due to the 
      extensive epigenetic regulation in germ line development, we hypothesize that 
      BDE-209 exposure impacts the statue of DNA methylation in spermatocytes in vitro. 
      Therefore, the mouse GC-2spd (GC-2) cells were used for the genome wide DNA 
      methylation analysis after treated with 32 mug/mL BDE-209 for 24 hr. The results 
      showed that BDE-209 caused genomic methylation changes with 32,083 differentially 
      methylated CpGs in GC-2 cells, including 16,164 (50.38%) hypermethylated and 
      15,919 (49.62%) hypomethylated sites. With integrated analysis of DNA methylation 
      data and functional enrichment, we found that BDE-209 might affect the functional 
      transcription in cell growth and sperm development by differential gene 
      methylation. qRT-PCR validation demonstrated the involvement of p53-dependent DNA 
      damage response in the GC-2 cells after BDE-209 exposure. In general, our 
      findings indicated that BDE-209-induced genome wide methylation changes could be 
      interrelated with reproductive dysfunction. This study might provide new insights 
      into the mechanisms of male reproductive toxicity under the environmental 
      exposure to BDE-209.
CI  - Copyright (c) 2021. Published by Elsevier B.V.
FAU - Li, Xiangyang
AU  - Li X
AD  - Department of Toxicology and Hygienic Chemistry, School of Public Health, Capital 
      Medical University, Beijing 100069, China; Beijing Key Laboratory of 
      Environmental Toxicology, Capital Medical University, Beijing 100069, China.
FAU - Zhang, Yue
AU  - Zhang Y
AD  - Department of Toxicology and Hygienic Chemistry, School of Public Health, Capital 
      Medical University, Beijing 100069, China; Beijing Key Laboratory of 
      Environmental Toxicology, Capital Medical University, Beijing 100069, China.
FAU - Dong, Xiaomin
AU  - Dong X
AD  - Experimental Center for basic medical teaching, Basic Medical Sciences, Capital 
      Medical University, Beijing 100069, China.
FAU - Zhou, Guiqing
AU  - Zhou G
AD  - Department of Toxicology and Hygienic Chemistry, School of Public Health, Capital 
      Medical University, Beijing 100069, China; Beijing Key Laboratory of 
      Environmental Toxicology, Capital Medical University, Beijing 100069, China.
FAU - Sang, Yujian
AU  - Sang Y
AD  - Department of Toxicology and Hygienic Chemistry, School of Public Health, Capital 
      Medical University, Beijing 100069, China; Beijing Key Laboratory of 
      Environmental Toxicology, Capital Medical University, Beijing 100069, China.
FAU - Gao, Leqiang
AU  - Gao L
AD  - Department of Toxicology and Hygienic Chemistry, School of Public Health, Capital 
      Medical University, Beijing 100069, China; Beijing Key Laboratory of 
      Environmental Toxicology, Capital Medical University, Beijing 100069, China.
FAU - Zhou, Xianqing
AU  - Zhou X
AD  - Department of Toxicology and Hygienic Chemistry, School of Public Health, Capital 
      Medical University, Beijing 100069, China; Beijing Key Laboratory of 
      Environmental Toxicology, Capital Medical University, Beijing 100069, China. 
      Electronic address: xqzhou2@163.com.
FAU - Sun, Zhiwei
AU  - Sun Z
AD  - Department of Toxicology and Hygienic Chemistry, School of Public Health, Capital 
      Medical University, Beijing 100069, China; Beijing Key Laboratory of 
      Environmental Toxicology, Capital Medical University, Beijing 100069, China.
LA  - eng
PT  - Journal Article
DEP - 20210424
PL  - Netherlands
TA  - J Environ Sci (China)
JT  - Journal of environmental sciences (China)
JID - 100967627
RN  - 0 (Flame Retardants)
RN  - 0 (Halogenated Diphenyl Ethers)
RN  - N80BQ29A0H (decabromobiphenyl ether)
SB  - IM
MH  - Animals
MH  - DNA Damage
MH  - *DNA Methylation
MH  - Epigenesis, Genetic
MH  - *Flame Retardants/toxicity
MH  - Germ Cells
MH  - Halogenated Diphenyl Ethers/toxicity
MH  - Male
MH  - Mice
OTO - NOTNLM
OT  - DNA damage response
OT  - DNA methylation
OT  - Decabrominated diphenyl ether (BDE-209)
OT  - Functional enrichment
OT  - GC-2spd
OT  - Germ cell development
EDAT- 2021/10/06 06:00
MHDA- 2021/10/07 06:00
CRDT- 2021/10/05 05:40
PHST- 2020/11/02 00:00 [received]
PHST- 2021/03/31 00:00 [revised]
PHST- 2021/04/01 00:00 [accepted]
PHST- 2021/10/05 05:40 [entrez]
PHST- 2021/10/06 06:00 [pubmed]
PHST- 2021/10/07 06:00 [medline]
AID - S1001-0742(21)00134-0 [pii]
AID - 10.1016/j.jes.2021.04.001 [doi]
PST - ppublish
SO  - J Environ Sci (China). 2021 Nov;109:161-170. doi: 10.1016/j.jes.2021.04.001. Epub 
      2021 Apr 24.