PMID- 34610214
OWN - NLM
STAT- MEDLINE
DCOM- 20211007
LR  - 20211007
IS  - 2691-1299 (Electronic)
IS  - 2691-1299 (Linking)
VI  - 1
IP  - 10
DP  - 2021 Oct
TI  - Multi-probe FISH Analysis of Immunophenotyped Chronic Lymphocytic Leukemia by 
      Imaging Flow Cytometry.
PG  - e260
LID - 10.1002/cpz1.260 [doi]
AB  - Imaging flow cytometry is an automated method that enables cells and fluorescent 
      signals to be visualized and quantified. Here, we describe a new imaging flow 
      cytometry method whereby fluorescence in situ hybridization (FISH) is integrated 
      with cell phenotyping. The method, called "immuno-flowFISH," provides an exciting 
      new dimension for the analysis of genomic changes in cytological samples (e.g., 
      blood, bone marrow). Cells are analyzed in suspension without any requirement for 
      prior cell isolation or separation. Multiple antibodies and FISH probes, each 
      with a unique fluorophore, can be added and many thousands of cells analyzed. 
      Specific cell populations are identified by their antigenic profile and then 
      analyzed for the presence of chromosomal defects. Immuno-flowFISH was applied to 
      the assessment of chronic lymphocytic leukemia (CLL), a mature B-cell neoplasm 
      where chromosomal abnormalities predict prognosis and treatment requirements. 
      This integrated immunophenotyping and multi-probe FISH strategy could detect both 
      structural and numerical chromosomal changes involving chromosomes 12 and 17 in 
      CLL cells. Given that many thousands of cells were analyzed and the leukemic 
      cells were positively identified by their immunophenotype, this multi-probe 
      method adds precision to the cytogenomic analysis of CLL. (c) 2021 Wiley 
      Periodicals LLC.
CI  - (c) 2021 Wiley Periodicals LLC.
FAU - Hui, Henry Y L
AU  - Hui HYL
AD  - Translational Cancer Pathology Laboratory, School of Biomedical Sciences (M504), 
      The University of Western Australia, Crawley, Western Australia, Australia.
FAU - Stanley, Jason
AU  - Stanley J
AD  - Translational Cancer Pathology Laboratory, School of Biomedical Sciences (M504), 
      The University of Western Australia, Crawley, Western Australia, Australia.
FAU - Clarke, Kathryn
AU  - Clarke K
AD  - Department of Haematology, Belfast City Hospital, Belfast, Northern Ireland, 
      United Kingdom.
FAU - Erber, Wendy N
AU  - Erber WN
AD  - Translational Cancer Pathology Laboratory, School of Biomedical Sciences (M504), 
      The University of Western Australia, Crawley, Western Australia, Australia.
AD  - PathWest Laboratory Medicine, Nedlands, Western Australia, Australia.
FAU - Fuller, Kathryn A
AU  - Fuller KA
AD  - Translational Cancer Pathology Laboratory, School of Biomedical Sciences (M504), 
      The University of Western Australia, Crawley, Western Australia, Australia.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Curr Protoc
JT  - Current protocols
JID - 101773894
SB  - IM
MH  - Chromosome Aberrations
MH  - Flow Cytometry
MH  - Humans
MH  - Immunophenotyping
MH  - In Situ Hybridization, Fluorescence
MH  - *Leukemia, Lymphocytic, Chronic, B-Cell/genetics
OTO - NOTNLM
OT  - CLL
OT  - chronic lymphocytic leukemia
OT  - fluorescence in situ hybridization
OT  - imaging flow cytometry
OT  - immunophenotype
EDAT- 2021/10/06 06:00
MHDA- 2021/10/08 06:00
CRDT- 2021/10/05 17:29
PHST- 2021/10/05 17:29 [entrez]
PHST- 2021/10/06 06:00 [pubmed]
PHST- 2021/10/08 06:00 [medline]
AID - 10.1002/cpz1.260 [doi]
PST - ppublish
SO  - Curr Protoc. 2021 Oct;1(10):e260. doi: 10.1002/cpz1.260.