PMID- 34614416
OWN - NLM
STAT- MEDLINE
DCOM- 20211203
LR  - 20211214
IS  - 1879-0631 (Electronic)
IS  - 0024-3205 (Linking)
VI  - 286
DP  - 2021 Dec 1
TI  - Evaluating the influence of Human Umbilical Cord Mesenchymal Stem Cells-derived 
      exosomes loaded with miR-3182 on metastatic performance of Triple Negative Breast 
      Cancer cells.
PG  - 120015
LID - S0024-3205(21)01002-X [pii]
LID - 10.1016/j.lfs.2021.120015 [doi]
AB  - AIMS: Deregulation of microRNA (miRNA) function has been linked to numerous human 
      cancers, such as Triple Negative Breast Cancer (TNBC). Exosomes, a subgroup of 
      extracellular vehicles (EVs), can efficiently deliver many different cargo types 
      to the target cell and have an extensive role in delivering therapeutic cargo for 
      treatment. The present study intended to interrogate the effects of exosomal 
      delivery of miR-3182 on TNBC cellular processes. MAIN METHODS: Human Umbilical 
      Cord Mesenchymal Stem Cells (HUCMSCs) were cultured and exosomes were isolated 
      and characterized using TEM, SEM, DLS, and Western blot. Exosomes were 
      transfected with miR-3182 and added to the treatment groups. The expression level 
      of miR-3182 and their target genes including mTOR and S6KB1 were evaluated using 
      RT-qPCR. The effects of miR-3182 loaded HUCMSC-exosomes treatment on the cellular 
      aspect of MDA-MB-231 cells including their viability, migration potency, cell 
      cycle status and apoptosis were investigated. KEY FINDINGS: According to the 
      results, exosomal miR-3182 significantly abolished cell proliferation and 
      migration (P < 0.05). miR-3182 loaded exosomes also induced apoptosis in TNBC 
      cells by down-regulating mTOR and S6KB1 genes (P < 0.05). SIGNIFICANCE: In 
      nutshell, miR-3182-loaded HUCMSC-exosomes can suppress TNBC invasion, suggesting 
      that exosomes containing miR-3182 could be a reliable therapeutic paradigm in 
      TNBC therapy.
CI  - Copyright (c) 2021 Elsevier Inc. All rights reserved.
FAU - Khazaei-Poul, Yalda
AU  - Khazaei-Poul Y
AD  - Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid 
      Beheshti University of Medical Sciences, Tehran, Iran. Electronic address: 
      ykhazaei@sbmu.ac.ir.
FAU - Shojaei, Samaneh
AU  - Shojaei S
AD  - Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid 
      Beheshti University of Medical Sciences, Tehran, Iran. Electronic address: 
      s.shojaei@sbmu.ac.ir.
FAU - Koochaki, Ameneh
AU  - Koochaki A
AD  - Cellular and Molecular Biology Research Center, Shahid Beheshti University of 
      Medical Sciences, Tehran, Iran. Electronic address: Koochaki@sbmu.ac.ir.
FAU - Ghanbarian, Hossein
AU  - Ghanbarian H
AD  - Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid 
      Beheshti University of Medical Sciences, Tehran, Iran. Electronic address: 
      ghanbarian@sbmu.ac.ir.
FAU - Mohammadi-Yeganeh, Samira
AU  - Mohammadi-Yeganeh S
AD  - Department of Biotechnology, School of Advanced Technologies in Medicine, Shahid 
      Beheshti University of Medical Sciences, Tehran, Iran; Cellular and Molecular 
      Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, 
      Iran. Electronic address: s.mohammadiyeganeh@sbmu.ac.ir.
LA  - eng
PT  - Journal Article
DEP - 20211003
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Apoptosis/genetics
MH  - Cell Differentiation
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - Down-Regulation
MH  - Exosomes/*metabolism
MH  - Humans
MH  - Mesenchymal Stem Cells/cytology/*metabolism
MH  - *Neoplasm Metastasis
MH  - TOR Serine-Threonine Kinases/genetics
MH  - Triple Negative Breast Neoplasms/*pathology
MH  - Umbilical Cord/cytology/*metabolism
OTO - NOTNLM
OT  - Exosome
OT  - Human Umbilical Cord Mesenchymal Stem Cells
OT  - S6KB1
OT  - Triple Negative Breast Cancer (TNBC)
OT  - mTOR
OT  - miR-3182
EDAT- 2021/10/07 06:00
MHDA- 2021/12/15 06:00
CRDT- 2021/10/06 20:07
PHST- 2021/05/05 00:00 [received]
PHST- 2021/09/28 00:00 [revised]
PHST- 2021/09/29 00:00 [accepted]
PHST- 2021/10/07 06:00 [pubmed]
PHST- 2021/12/15 06:00 [medline]
PHST- 2021/10/06 20:07 [entrez]
AID - S0024-3205(21)01002-X [pii]
AID - 10.1016/j.lfs.2021.120015 [doi]
PST - ppublish
SO  - Life Sci. 2021 Dec 1;286:120015. doi: 10.1016/j.lfs.2021.120015. Epub 2021 Oct 3.