PMID- 34615899
OWN - NLM
STAT- MEDLINE
DCOM- 20211228
LR  - 20211228
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 11
IP  - 1
DP  - 2021 Oct 6
TI  - Transcriptomic profiling and functional prediction reveal aberrant expression of 
      circular RNAs during osteogenic differentiation in human umbilical cord 
      mesenchymal stromal cells.
PG  - 19881
LID - 10.1038/s41598-021-98470-2 [doi]
LID - 19881
AB  - Circular RNAs (circRNAs) are crucial elements of non-coding RNA, that regulate 
      various biological processes. To date, expression patterns and functional roles 
      of circRNAs during osteogenic differentiation of human umbilical cord mesenchymal 
      stromal cells (hUCMSCs) remain unknown. In this study, we analyzed RNA-sequence 
      data to reveal expression profiles of circRNAs during osteogenesis of hUCMSCs, 
      then elucidated the underlying mechanisms of action. We identified a total of 
      5457 circRNAs in hUCMSCs, of which 34 and 33 were upregulated and downregulated, 
      respectively. We applied Gene Ontology and Kyoto Encyclopedia of Genes and 
      Genomes analyses to determine functions and related pathways of differentially 
      expressed circRNAs. Moreover, we applied bioinformatics tools to construct 
      competing endogenous RNA networks, comprising 10 circRNAs, 46 micro RNAs and 413 
      mRNAs. Furthermore, we predicted protein-coding potential of the upregulated 
      circRNAs then constructed a co-expression network comprising the top 5 
      upregulated circRNAs and 75 RNA-binding proteins. Next, we validated 6 
      differentially-expressed circRNAs and found that overexpressing circ-CTTN could 
      promote osteogenesis of hUCMSCs. Overall, our findings indicate that clusters of 
      circRNAs are aberrantly expressed in hUCMSCs during osteogenic differentiation, 
      hence lay a foundation for future research into promoting hUCMSCs osteogenic 
      differentiation and bone regeneration.
CI  - (c) 2021. The Author(s).
FAU - Su, Cheng
AU  - Su C
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, West China Hospital of Stomatology, Department of Oral Prosthodontics, 
      Sichuan University, Sichuan, 610041, China.
FAU - Zheng, Xiao
AU  - Zheng X
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, West China Hospital of Stomatology, Department of Oral Prosthodontics, 
      Sichuan University, Sichuan, 610041, China.
FAU - He, Yanjin
AU  - He Y
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, West China Hospital of Stomatology, Department of Oral Prosthodontics, 
      Sichuan University, Sichuan, 610041, China.
FAU - Long, Li
AU  - Long L
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, West China Hospital of Stomatology, Department of Oral Prosthodontics, 
      Sichuan University, Sichuan, 610041, China.
FAU - Chen, Wenchuan
AU  - Chen W
AD  - State Key Laboratory of Oral Diseases, National Clinical Research Center for Oral 
      Diseases, West China Hospital of Stomatology, Department of Oral Prosthodontics, 
      Sichuan University, Sichuan, 610041, China. hxkqcwc@scu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20211006
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Circular)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Cell Differentiation/*genetics
MH  - Cells, Cultured
MH  - Computational Biology/methods
MH  - *Gene Expression Profiling
MH  - Gene Expression Regulation
MH  - Gene Ontology
MH  - Gene Regulatory Networks
MH  - High-Throughput Nucleotide Sequencing
MH  - Humans
MH  - Mesenchymal Stem Cells/cytology/*metabolism
MH  - MicroRNAs/genetics
MH  - Osteogenesis/*genetics
MH  - RNA, Circular/*genetics
MH  - RNA, Messenger/genetics
MH  - *Transcriptome
MH  - Umbilical Cord/*cytology
PMC - PMC8494929
COIS- The authors declare no competing interests.
EDAT- 2021/10/08 06:00
MHDA- 2021/12/29 06:00
PMCR- 2021/10/06
CRDT- 2021/10/07 06:17
PHST- 2021/04/07 00:00 [received]
PHST- 2021/09/06 00:00 [accepted]
PHST- 2021/10/07 06:17 [entrez]
PHST- 2021/10/08 06:00 [pubmed]
PHST- 2021/12/29 06:00 [medline]
PHST- 2021/10/06 00:00 [pmc-release]
AID - 10.1038/s41598-021-98470-2 [pii]
AID - 98470 [pii]
AID - 10.1038/s41598-021-98470-2 [doi]
PST - epublish
SO  - Sci Rep. 2021 Oct 6;11(1):19881. doi: 10.1038/s41598-021-98470-2.