PMID- 3461781
OWN - NLM
STAT- MEDLINE
DCOM- 19860917
LR  - 20190501
IS  - 0264-6021 (Print)
IS  - 1470-8728 (Electronic)
IS  - 0264-6021 (Linking)
VI  - 235
IP  - 1
DP  - 1986 Apr 1
TI  - Properties of neurofilament protein kinase.
PG  - 283-9
AB  - Neurofilament (NF) protein kinase, partially purified from NF preparations 
      [Toru-Delbauffe & Pierre (1983) FEBS Lett. 162, 230-234], was found to be 
      distinct from both the casein kinase present in NFs and the cyclic AMP-dependent 
      protein kinase which is able to phosphorylate NFs. NF-kinase phosphorylated the 
      three NF protein components. The amount of phosphate incorporated per molecule 
      was higher for NF 200 than for NF 145 and NF 68. Other proteins present in the NF 
      preparations were also used as NF-kinase substrates. Two of them might correspond 
      to the myelin basic proteins with Mr values of 18,000 and 21,000. Four other 
      substrates in the NF preparation were not identified (respective Mr values 
      53,000, 55,000, 65,000 and greater than 300,000). NF kinase also phosphorylated 
      two additional brain-cell cytoskeletal elements: GFAp and vimentin. Casein, 
      histones and phosvitin, currently used as substrates for protein kinase assays, 
      were very poor phosphate acceptors. Half-maximal NF-kinase activity was obtained 
      at an NF protein concentration of about 0.25 mg/ml in heated, salt-washed, NF 
      preparations. The specific activity was about 5 pmol of 32P incorporated/min per 
      microgram of NF kinase preparation protein. ATP was a phospho-group donor (Km 8 X 
      10(-5) M), but GTP was not. NF-kinase activity remained stable at 65 degrees C 
      for more than 1 h. The enzyme was not degraded by storage at -20 degrees C for 
      several months in a buffer containing 50% (w/v) sucrose. Maximal activity was 
      obtained with 5 mM-Mg2+ (Mg2+ could be replaced by Co2+); Zn2+ and Cu2+ inhibited 
      the reaction. NF-kinase was not dependent on cyclic AMP, cyclic GMP, Ca2+ or Ca2+ 
      plus dioleoylglycerol and phosphatidylserine.
FAU - Toru-Delbauffe, D
AU  - Toru-Delbauffe D
FAU - Pierre, M
AU  - Pierre M
FAU - Osty, J
AU  - Osty J
FAU - Chantoux, F
AU  - Chantoux F
FAU - Francon, J
AU  - Francon J
LA  - eng
PT  - Journal Article
PL  - England
TA  - Biochem J
JT  - The Biochemical journal
JID - 2984726R
RN  - 0 (Cations, Divalent)
RN  - 0 (Nerve Tissue Proteins)
RN  - EC 2.7.- (Protein Kinases)
RN  - EC 2.7.11.1 (Casein Kinases)
RN  - I38ZP9992A (Magnesium)
SB  - IM
MH  - Animals
MH  - Casein Kinases
MH  - Cations, Divalent/pharmacology
MH  - Cattle
MH  - Cytoskeleton/*enzymology
MH  - Electrophoresis, Polyacrylamide Gel
MH  - Intermediate Filaments/drug effects/*enzymology
MH  - Magnesium/pharmacology
MH  - Nerve Tissue Proteins/metabolism
MH  - Phosphorylation
MH  - Protein Kinases/*metabolism
MH  - Substrate Specificity
MH  - Temperature
PMC - PMC1146679
EDAT- 1986/04/01 00:00
MHDA- 1986/04/01 00:01
PMCR- 1986/04/01
CRDT- 1986/04/01 00:00
PHST- 1986/04/01 00:00 [pubmed]
PHST- 1986/04/01 00:01 [medline]
PHST- 1986/04/01 00:00 [entrez]
PHST- 1986/04/01 00:00 [pmc-release]
AID - 10.1042/bj2350283 [doi]
PST - ppublish
SO  - Biochem J. 1986 Apr 1;235(1):283-9. doi: 10.1042/bj2350283.