PMID- 34618048
OWN - NLM
STAT- MEDLINE
DCOM- 20220310
LR  - 20220310
IS  - 1532-2548 (Electronic)
IS  - 0032-0889 (Print)
IS  - 0032-0889 (Linking)
VI  - 187
IP  - 3
DP  - 2021 Nov 3
TI  - Recycling of the major thylakoid lipid MGDG and its role in lipid homeostasis in 
      Chlamydomonas reinhardtii.
PG  - 1341-1356
LID - 10.1093/plphys/kiab340 [doi]
AB  - Monogalactosyldiacylglycerol (MGDG), the most abundant lipid in thylakoid 
      membranes, is involved in photosynthesis and chloroplast development. MGDG lipase 
      has an important role in lipid remodeling in Chlamydomonas reinhardtii. However, 
      the process related to turnover of the lysogalactolipid that results from MGDG 
      degradation, monogalactosylmonoacylglycerol (MGMG), remains to be clarified. Here 
      we identified a homolog of Arabidopsis thaliana lysophosphatidylcholine 
      acyltransferase (LPCAT) and characterized two independent knockdown (KD) alleles 
      in C. reinhardtii. The enzyme designated as C. reinhardtiiLysolipid 
      Acyltransferase 1 (CrLAT1) has a conserved membrane-bound O-acyl transferase 
      domain. LPCAT from Arabidopsis has a key role in deacylation of 
      phosphatidylcholine (PC). Chlamydomonas reinhardtii, however, lacks PC, and thus 
      we hypothesized that CrLAT1 has some other important function in major lipid flow 
      in this organism. In the CrLAT1 KD mutants, the amount of MGMG was increased, but 
      triacylglycerols (TAGs) were decreased. The proportion of more saturated 18:1 (9) 
      MGDG was lower in the KD mutants than in their parental strain, CC-4533. In 
      contrast, the proportion of MGMG has decreased in the CrLAT1 overexpression (OE) 
      mutants, and the proportion of 18:1 (9) MGDG was higher in the OE mutants than in 
      the empty vector control cells. Thus, CrLAT1 is involved in the recycling of MGDG 
      in the chloroplast and maintains lipid homeostasis in C. reinhardtii.
CI  - (c) The Author(s) 2021. Published by Oxford University Press on behalf of American 
      Society of Plant Biologists.
FAU - Iwai, Masako
AU  - Iwai M
AUID- ORCID: 0000-0001-6121-7543
AD  - School of Life Science and Technology, Tokyo Institute of Technology, Yokohama 
      226-8501, Japan.
FAU - Yamada-Oshima, Yui
AU  - Yamada-Oshima Y
AD  - School of Life Science and Technology, Tokyo Institute of Technology, Yokohama 
      226-8501, Japan.
FAU - Asami, Kota
AU  - Asami K
AD  - School of Life Science and Technology, Tokyo Institute of Technology, Yokohama 
      226-8501, Japan.
FAU - Kanamori, Takashi
AU  - Kanamori T
AD  - School of Life Science and Technology, Tokyo Institute of Technology, Yokohama 
      226-8501, Japan.
FAU - Yuasa, Hideya
AU  - Yuasa H
AUID- ORCID: 0000-0002-2864-3185
AD  - School of Life Science and Technology, Tokyo Institute of Technology, Yokohama 
      226-8501, Japan.
FAU - Shimojima, Mie
AU  - Shimojima M
AUID- ORCID: 0000-0003-3172-8638
AD  - School of Life Science and Technology, Tokyo Institute of Technology, Yokohama 
      226-8501, Japan.
FAU - Ohta, Hiroyuki
AU  - Ohta H
AUID- ORCID: 0000-0002-1068-3242
AD  - School of Life Science and Technology, Tokyo Institute of Technology, Yokohama 
      226-8501, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Plant Physiol
JT  - Plant physiology
JID - 0401224
RN  - 0 (Galactolipids)
RN  - 0 (monogalactosyldiacylglycerol)
SB  - IM
MH  - Chlamydomonas reinhardtii/*metabolism
MH  - Galactolipids/*metabolism
MH  - *Homeostasis
MH  - *Lipid Metabolism
MH  - Thylakoids/*metabolism
PMC - PMC8566231
EDAT- 2021/10/08 06:00
MHDA- 2022/03/11 06:00
PMCR- 2021/07/23
CRDT- 2021/10/07 12:25
PHST- 2021/06/14 00:00 [received]
PHST- 2021/06/24 00:00 [accepted]
PHST- 2021/10/08 06:00 [pubmed]
PHST- 2022/03/11 06:00 [medline]
PHST- 2021/10/07 12:25 [entrez]
PHST- 2021/07/23 00:00 [pmc-release]
AID - 6326810 [pii]
AID - kiab340 [pii]
AID - 10.1093/plphys/kiab340 [doi]
PST - ppublish
SO  - Plant Physiol. 2021 Nov 3;187(3):1341-1356. doi: 10.1093/plphys/kiab340.