PMID- 34618611
OWN - NLM
STAT- MEDLINE
DCOM- 20220223
LR  - 20220428
IS  - 1532-2513 (Electronic)
IS  - 0892-3973 (Linking)
VI  - 43
IP  - 6
DP  - 2021 Dec
TI  - Capillin protects against non-alcoholic steatohepatitis through suppressing NLRP3 
      inflammasome activation and oxidative stress.
PG  - 778-789
LID - 10.1080/08923973.2021.1984520 [doi]
AB  - BACKGROUND: Non-alcoholic steatohepatitis (NASH) is an extreme form of 
      non-alcoholic fatty liver disease. The present study concentrated on the role of 
      Capillin, a polyacetylene compound isolated from Artemisia capillaris Thunb., in 
      NASH development. MATERIALS AND METHODS: Palmitic acid (PA) was treated with 
      FL83B hepatocytes, and high-fat diet was given to mouse to construct the NASH 
      model in vivo. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide 
      (MTT) method, flow cytometry, and terminal deoxynucleotidyl transferase-mediated 
      dUTP nick end labeling (TUNEL) assay were carried out to measure the viability 
      and apoptosis of FL83B hepatocytes. Quantitative reverse transcription-polymerase 
      chain reaction (qRT-PCR) was performed to measure the mRNA expressions of 
      infiltration markers (Cd11c, Ccr2, and Ly6c), fibrosis genes (Tgfbeta1, Col1a1, and 
      Timp1), and alpha-smooth muscle actin (alpha-SMA). Western blot, immunofluorescence, 
      and Enzyme-linked immunosorbent assay (ELISA) were implemented to examine the 
      proteins of Caspase-3, Bcl2, Nrf2, HO-1, NLRP3, ASC, and Caspase-1, the ROS 
      level, and oxidative stress markers (MDA, GSH-ST, SOD, and GSH-Px), and the lipid 
      peroxidation level, respectively. Moreover, HE staining was manipulated to 
      observe the histopathological changes in liver tissue. RESULTS: Capillin hampered 
      PA-mediated hepatocytes apoptosis and enhanced cell viability. Furthermore, 
      Capillin suppressed PA-mediated oxidative stress in hepatocytes, promoted 
      Nrf2/HO-1 expression, and repressed NLRP3-ASC-Caspase1 inflammasome. The in vivo 
      studies indicated that Capillin vigorously improves liver fat accumulation, 
      oxidative stress, and liver injury in NASH mice. Mechanistically, Capillin 
      repressed NLRP3-ASC-Caspase1 inflammasome and up-regulated the Nrf2-HO-1 pathway 
      in the liver. CONCLUSION: Capillin ameliorates hepatocyte injury by dampening 
      oxidative stress and repressing NLRP3 inflammasome in NASH mice.
FAU - Li, Bin
AU  - Li B
AD  - Department of Hepatology, Zaozhuang Hospital of Chinese Medicine, Zaozhuang, 
      Shandong, China.
FAU - Wang, Rui
AU  - Wang R
AD  - Department of Gastroenterology, Zaozhuang Hospital of Chinese Medicine, 
      Zaozhuang, Shandong, China.
FAU - Wang, Lei
AU  - Wang L
AD  - Department of Hepatology, Zaozhuang Hospital of Chinese Medicine, Zaozhuang, 
      Shandong, China.
FAU - Zhang, Gucheng
AU  - Zhang G
AD  - Department of Hepatology, Zaozhuang Hospital of Chinese Medicine, Zaozhuang, 
      Shandong, China.
FAU - Zhang, Yang
AU  - Zhang Y
AD  - Department of Hepatology, Zaozhuang Municipal Hospital, Zaozhuang, Shandong, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20211007
PL  - England
TA  - Immunopharmacol Immunotoxicol
JT  - Immunopharmacology and immunotoxicology
JID - 8800150
RN  - 0 (Diynes)
RN  - 0 (NLR Family, Pyrin Domain-Containing 3 Protein)
RN  - 0 (Nlrp3 protein, mouse)
RN  - 0 (Plant Extracts)
RN  - 495-74-9 (capillin)
SB  - IM
MH  - Animals
MH  - Diet, High-Fat/adverse effects
MH  - Diynes/pharmacology/*therapeutic use
MH  - Dose-Response Relationship, Drug
MH  - Hepatocytes/drug effects/metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - NLR Family, Pyrin Domain-Containing 3 Protein/*antagonists & 
      inhibitors/metabolism
MH  - Non-alcoholic Fatty Liver Disease/metabolism/*prevention & control
MH  - Oxidative Stress/*drug effects/physiology
MH  - Plant Extracts/pharmacology/*therapeutic use
OTO - NOTNLM
OT  - Capillin
OT  - NLRP3
OT  - apoptosis
OT  - non-alcoholic steatohepatitis
OT  - oxidative stress
EDAT- 2021/10/08 06:00
MHDA- 2022/02/24 06:00
CRDT- 2021/10/07 17:16
PHST- 2021/10/08 06:00 [pubmed]
PHST- 2022/02/24 06:00 [medline]
PHST- 2021/10/07 17:16 [entrez]
AID - 10.1080/08923973.2021.1984520 [doi]
PST - ppublish
SO  - Immunopharmacol Immunotoxicol. 2021 Dec;43(6):778-789. doi: 
      10.1080/08923973.2021.1984520. Epub 2021 Oct 7.