PMID- 34619260 OWN - NLM STAT- MEDLINE DCOM- 20211206 LR - 20211214 IS - 1879-1298 (Electronic) IS - 0045-6535 (Linking) VI - 288 IP - Pt 1 DP - 2022 Feb TI - Urinary and seminal plasma concentrations of phthalate metabolites in relation to spermatogenesis-related miRNA106a among men from an infertility clinic. PG - 132464 LID - S0045-6535(21)02936-2 [pii] LID - 10.1016/j.chemosphere.2021.132464 [doi] AB - Studies indicate that phthalates can disrupt spermatogenesis and lead to the reduction of semen quality. However, the underlying mechanisms remain unclear. This study aimed to examine the associations of phthalate exposures as individual chemicals and mixtures with spermatogenesis-related miRNA106a. We detected eight phthalate metabolites in repeated urine samples and a single seminal plasma specimen among 111 men from an infertility clinic in Wuhan, China. Spermatogenesis-related miRNA106a was measured in seminal plasma. We used multivariable linear regression and Bayesian kernel machine regression (BKMR) models to separately evaluate the associations of phthalate metabolites as individual chemicals and mixtures with spermatogenesis-related miRNA106a. Elevated tertiles of urinary mono (2-ethylhexyl) phthalate (MEHP) was associated with decreased miRNA106a (-61.71%; 95%CI: 81.92, -18.93% for the highest vs. lowest tertile; P for trend = 0.01). Similarly, an inverse exposure-response relationship between seminal plasma MEHP concentrations and miRNA106a was also observed (-59.44%; 95%CI: 79.19, -20.95% for the highest vs. lowest tertile; P for trend = 0.01). The BKMR models showed that the mixtures of seminal plasma phthalate metabolites were associated with decreased miRNA106a when the chemical mixtures were >/=35th percentile compared to their medians. Nonlinear associations with miRNA106a were estimated for urinary and seminal plasma MEHP while fixing other phthalate metabolites at their medians. Our findings suggest that mixtures of phthalate metabolites in seminal plasma were negatively associated with spermatogenesis-related miRNA106a, and individual MEHP was the major contributor to the adverse effects. CI - Copyright (c) 2021 Elsevier Ltd. All rights reserved. FAU - Cui, Fei-Peng AU - Cui FP AD - Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, PR China; Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health (incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, PR China. FAU - Liu, Chong AU - Liu C AD - Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, PR China; Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health (incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, PR China. FAU - Deng, Yan-Ling AU - Deng YL AD - Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, PR China; Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health (incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, PR China. FAU - Chen, Pan-Pan AU - Chen PP AD - Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, PR China; Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health (incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, PR China. FAU - Miao, Yu AU - Miao Y AD - Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, PR China; Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health (incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, PR China. FAU - Luo, Qiong AU - Luo Q AD - Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, PR China; Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health (incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, PR China. FAU - Zhang, Min AU - Zhang M AD - Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, PR China; Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health (incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, PR China. FAU - Yang, Pan AU - Yang P AD - Department of Occupational and Environmental Health, School of Basic Medicine and Public Health, Jinan University, Guangzhou, Guangdong, PR China. FAU - Wang, Yi-Xin AU - Wang YX AD - Department of Nutrition, Harvard T.H. Chan School of Public Health, Boston, MA, USA. FAU - Lu, Wen-Qing AU - Lu WQ AD - Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, PR China; Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health (incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, PR China. FAU - Zeng, Qiang AU - Zeng Q AD - Department of Occupational and Environmental Health, School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, PR China; Key Laboratory of Environment and Health, Ministry of Education & Ministry of Environmental Protection, and State Key Laboratory of Environmental Health (incubating), School of Public Health, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei, PR China. Electronic address: zengqiang506@163.com. LA - eng PT - Journal Article DEP - 20211004 PL - England TA - Chemosphere JT - Chemosphere JID - 0320657 RN - 0 (Phthalic Acids) RN - 6O7F7IX66E (phthalic acid) SB - IM MH - Bayes Theorem MH - Environmental Exposure MH - Fertility Clinics MH - Humans MH - Male MH - *Phthalic Acids MH - *Semen MH - Semen Analysis MH - Spermatogenesis OTO - NOTNLM OT - Bayesian kernel machine regression OT - MiRNA106a OT - Phthalate mixtures OT - Seminal plasma OT - Urine EDAT- 2021/10/08 06:00 MHDA- 2021/12/15 06:00 CRDT- 2021/10/07 20:16 PHST- 2021/08/25 00:00 [received] PHST- 2021/09/29 00:00 [revised] PHST- 2021/10/01 00:00 [accepted] PHST- 2021/10/08 06:00 [pubmed] PHST- 2021/12/15 06:00 [medline] PHST- 2021/10/07 20:16 [entrez] AID - S0045-6535(21)02936-2 [pii] AID - 10.1016/j.chemosphere.2021.132464 [doi] PST - ppublish SO - Chemosphere. 2022 Feb;288(Pt 1):132464. doi: 10.1016/j.chemosphere.2021.132464. Epub 2021 Oct 4.