PMID- 34623595
OWN - NLM
STAT- MEDLINE
DCOM- 20220225
LR  - 20220225
IS  - 1573-4978 (Electronic)
IS  - 0301-4851 (Linking)
VI  - 48
IP  - 11
DP  - 2021 Nov
TI  - Cellular uptake and apoptotic properties of gemini curcumin in gastric cancer 
      cells.
PG  - 7215-7222
LID - 10.1007/s11033-021-06713-2 [doi]
AB  - INTRODUCTION: Curcumin is a polyphenolic natural compound, which has demonstrated 
      to possess antioxidant, anti-inflammatory, and anticancer effects in vitro & in 
      vivo. However, its applicability in cancer therapy has been limited due to its 
      poor cellular uptake. Here, we aimed to evaluate the anticancer effect of novel 
      gemini curcumin (Gemini-Cur) on the gastric cancer AGS cells. METHOD: The AGS 
      cancerous and HFF-2 non-cancerous cells were treated with Gemini-Cur and curcumin 
      (Cur) in a time- and dose-dependent manner. Cellular toxicity was studied using 
      MTT, fluorescence microscopy, annexin V/FITC, and cell cycle assays. 
      Additionally, real-time PCR and western blotting were employed to evaluate the 
      expression of Bax, Bcl-2 and survivin genes. RESULTS: Our data indicated that 
      Gemini-Cur is significantly taken into AGS cells compared to Cur. Moreover, the 
      viability of Gemini-Cur treated cells was significantly reduced in a time- and 
      dose-dependent manner (p < 0.001). Gemini-Cur compound induced G2/M cell cycle 
      arrest that was followed by apoptosis in a time-dependent manner (p < 0.0001). 
      DISCUSSION: Taken together, our findings support the idea that Gemini-Cur has the 
      potential to be considered as an anticancer agent.
CI  - (c) 2021. The Author(s), under exclusive licence to Springer Nature B.V.
FAU - Emami, Ali
AU  - Emami A
AD  - Department of Animal Biology, School of Natural Sciences, University of Tabriz, 
      51555, Tabriz, Iran.
FAU - Babaei, Esmaeil
AU  - Babaei E
AUID- ORCID: 0000-0002-1603-5166
AD  - Department of Animal Biology, School of Natural Sciences, University of Tabriz, 
      51555, Tabriz, Iran. babaei@tabrizu.ac.ir.
FAU - Nagishbandi, Alaadin
AU  - Nagishbandi A
AD  - Department of Pharmacognosy, College of Pharmacy, Hawler Medical University, 
      Kurdistan Region, Erbil, Iraq.
FAU - Azeez, Hewa Jalal
AU  - Azeez HJ
AD  - Department of Animal Biology, School of Natural Sciences, University of Tabriz, 
      51555, Tabriz, Iran.
FAU - Feizi, Mohammad Ali Hosseinpour
AU  - Feizi MAH
AD  - Department of Animal Biology, School of Natural Sciences, University of Tabriz, 
      51555, Tabriz, Iran.
FAU - Golizadeh, Ashraf
AU  - Golizadeh A
AD  - Department of Animal Biology, School of Natural Sciences, University of Tabriz, 
      51555, Tabriz, Iran.
LA  - eng
PT  - Journal Article
DEP - 20211008
PL  - Netherlands
TA  - Mol Biol Rep
JT  - Molecular biology reports
JID - 0403234
RN  - IT942ZTH98 (Curcumin)
SB  - IM
MH  - Apoptosis/*drug effects
MH  - Cell Line, Tumor
MH  - Cell Proliferation/*drug effects
MH  - Cell Survival/drug effects
MH  - *Curcumin/pharmacokinetics/pharmacology
MH  - Humans
MH  - *Stomach Neoplasms/drug therapy/metabolism/pathology
OTO - NOTNLM
OT  - Apoptosis
OT  - Bax/Bcl-2 ratio
OT  - Cell cycle
OT  - Gastric cancer
OT  - Gemini curcumin
OT  - Western blotting
EDAT- 2021/10/09 06:00
MHDA- 2022/02/26 06:00
CRDT- 2021/10/08 12:26
PHST- 2020/12/03 00:00 [received]
PHST- 2021/09/19 00:00 [accepted]
PHST- 2021/10/09 06:00 [pubmed]
PHST- 2022/02/26 06:00 [medline]
PHST- 2021/10/08 12:26 [entrez]
AID - 10.1007/s11033-021-06713-2 [pii]
AID - 10.1007/s11033-021-06713-2 [doi]
PST - ppublish
SO  - Mol Biol Rep. 2021 Nov;48(11):7215-7222. doi: 10.1007/s11033-021-06713-2. Epub 
      2021 Oct 8.