PMID- 34624531
OWN - NLM
STAT- MEDLINE
DCOM- 20211020
LR  - 20211020
IS  - 1090-2414 (Electronic)
IS  - 0147-6513 (Linking)
VI  - 226
DP  - 2021 Dec 15
TI  - Lead impaired immune function and tissue integrity in yellow catfish (Peltobargus 
      fulvidraco) by mediating oxidative stress, inflammatory response and apoptosis.
PG  - 112857
LID - S0147-6513(21)00969-6 [pii]
LID - 10.1016/j.ecoenv.2021.112857 [doi]
AB  - Lead (Pb) widely exists in the water environment and has severe toxic effects on 
      aquatic organisms. The yellow catfish (Pelteobagrus fulvidraco) is one of the 
      most important commercial species in China, and moreover, its natural populations 
      are declining with the degradation of environmental water quality. However, 
      little is known about the toxic effects of Pb on its immune organs. This study 
      was performed to determine waterborne Pb exposure on bioaccumulation, 
      histomorphology, antioxidant status, apoptotic and immune response in the head 
      kidney and spleen of yellow catfish. Experimental fish were randomly allocated 
      into twelve tanks (3 tanks per group), and the Pb concentrations of the four 
      groups were 0, 5, 50, and 500 mug/L, respectively. The results reflected that the 
      Pb bioaccumulation of the head kidney and spleen increased with increasing Pb 
      exposure dose and time. Severe histological alterations in the head kidney and 
      spleen were observed at concentration 500 ug/L. With increasing Pb exposure 
      concentrations, the plasma activity of superoxide dismutase (SOD) and catalase 
      (CAT) significantly increased after exposure 7 days and 14 days, and the levels 
      significantly decreased after exposure 28 days. The change trend of glutathione 
      (GSH) levels was opposite to that of SOD and CAT at corresponding exposure time. 
      The plasma malondialdehyde (MDA) levels together with the activities of plasma 
      alkaline phosphatase (AKP) and acid phosphatase (ACP) increased significantly 
      with the increasing Pb concentrations. In contrast, the levels of lysozyme (LYZ), 
      complement 3 (C3) and immunoglobulin M (IgM) decreased significantly with 
      increasing Pb concentrations. Moreover, Pb exposure induced transcriptional 
      upregulation of heat shock protein 70 (hsp70), metallothionein (mt), sod, cat, 
      interleukin-10 (il-10), transforming growth factor-beta (tgf-beta), and tumor necrosis 
      factor-alpha (tnf-alpha), bcl-2-associated X protein (bax), and cysteinyl aspartate 
      specific proteinase -9 (caspase-9), genes in the head kidney and spleen tissues, 
      while downregulating the levels of the lyz, c3, igm and B-cell lymphoma-2 (bcl-2) 
      genes. Our data provide evidence that Pb impaired immune function and tissue 
      integrity in yellow catfish through oxidative stress, inflammatory and apoptosis, 
      and the results can serve as reference data to better protect water environments 
      from Pb eco-toxicants.
CI  - Copyright (c) 2021 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Guo, Jie
AU  - Guo J
AD  - Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China.
FAU - Pu, Yan
AU  - Pu Y
AD  - Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Wuhan 430223, China; School of Life Sciences, Southwest University, Chongqing 
      400715, China.
FAU - Zhong, Liqiao
AU  - Zhong L
AD  - Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Wuhan 430223, China.
FAU - Wang, Ke
AU  - Wang K
AD  - Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Wuhan 430223, China.
FAU - Duan, Xinbin
AU  - Duan X
AD  - Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Wuhan 430223, China.
FAU - Chen, Daqing
AU  - Chen D
AD  - Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, China; 
      Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Sciences, 
      Wuhan 430223, China. Electronic address: chdq@yfi.ac.cn.
LA  - eng
PT  - Journal Article
DEP - 20211005
PL  - Netherlands
TA  - Ecotoxicol Environ Saf
JT  - Ecotoxicology and environmental safety
JID - 7805381
RN  - 0 (Fish Proteins)
RN  - 2P299V784P (Lead)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - *Catfishes/metabolism
MH  - Fish Proteins/metabolism
MH  - Immunity, Innate
MH  - Lead/toxicity
MH  - Oxidative Stress
OTO - NOTNLM
OT  - Apoptosis
OT  - Immunosuppression
OT  - Inflammatory
OT  - Oxidative stress
OT  - Pb
OT  - Pelteobagrus fulvidraco
EDAT- 2021/10/09 06:00
MHDA- 2021/10/21 06:00
CRDT- 2021/10/08 20:17
PHST- 2021/07/27 00:00 [received]
PHST- 2021/09/11 00:00 [revised]
PHST- 2021/09/14 00:00 [accepted]
PHST- 2021/10/09 06:00 [pubmed]
PHST- 2021/10/21 06:00 [medline]
PHST- 2021/10/08 20:17 [entrez]
AID - S0147-6513(21)00969-6 [pii]
AID - 10.1016/j.ecoenv.2021.112857 [doi]
PST - ppublish
SO  - Ecotoxicol Environ Saf. 2021 Dec 15;226:112857. doi: 
      10.1016/j.ecoenv.2021.112857. Epub 2021 Oct 5.