PMID- 34630696
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211012
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Print)
IS  - 1792-0981 (Linking)
VI  - 22
IP  - 5
DP  - 2021 Nov
TI  - Downregulation of MIAT reduces the proliferation and migratory and invasive 
      abilities of retinoblastoma cells by sponging miR-665 and regulating LASP1.
PG  - 1342
LID - 10.3892/etm.2021.10777 [doi]
LID - 1342
AB  - Long non-coding RNAs (lncRNAs) can function as onco-lncRNAs in several types of 
      human cancer, including retinoblastoma (Rb). The present study investigated the 
      potential role and regulatory mechanism of the lncRNA myocardial 
      infarction-associated transcript (MIAT) in Rb. To do so, the expression levels of 
      MIAT, microRNA (miR)-665, and LIM and SH3 protein 1 (LASP1) in Rb tissues from 
      patients or Rb cells were analysed using reverse transcription quantitative PCR. 
      The interactions between miR-665 and MIAT/LASP1 were confirmed by the 
      dual-luciferase reporter assay. MTT, Transwell (to assess migration and invasion) 
      and western blotting assays were used to explore the functions of the 
      MIAT/miR-665/LASP1 axis on Rb progression in vitro. The results of the present 
      study indicated that MIAT targeted miR-665. In Rb tissues and cell lines, high 
      expression of MIAT was observed, whereas miR-665 was downregulated in Rb tissues. 
      Furthermore, the proliferation and migratory and invasive abilities of Rb Y79 and 
      HXO-RB44 cells were decreased following MIAT downregulation or miR-665 
      overexpression. In addition, LASP1 was identified as a target gene of miR-665. 
      Both the decreased expression of miR-665 and the elevated expression of LASP1 
      reversed the suppressive effects of MIAT knockdown on the proliferation and 
      migratory and invasive abilities of Y79 cells. Furthermore, MIAT silencing 
      attenuated the development of Rb by regulating the miR-665/LASP1 axis. Taken 
      together, these findings suggested that MIAT may be considered as a possible 
      therapeutic target for Rb.
CI  - Copyright (c) 2020, Spandidos Publications.
FAU - Xu, Xiabing
AU  - Xu X
AD  - Department of Ophthalmology, No. 215 Hospital of Shaanxi Nuclear Industry, 
      Xianyang, Shaanxi 712000, P.R. China.
FAU - Zhao, Yadong
AU  - Zhao Y
AD  - Department of Ophthalmology, No. 215 Hospital of Shaanxi Nuclear Industry, 
      Xianyang, Shaanxi 712000, P.R. China.
FAU - Duan, Gang
AU  - Duan G
AD  - Department of Ophthalmology, No. 215 Hospital of Shaanxi Nuclear Industry, 
      Xianyang, Shaanxi 712000, P.R. China.
FAU - Du, Bo
AU  - Du B
AD  - Department of Ophthalmology, No. 215 Hospital of Shaanxi Nuclear Industry, 
      Xianyang, Shaanxi 712000, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20210922
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC8495550
OTO - NOTNLM
OT  - LIM and SH3 protein 1
OT  - long non-coding RNA myocardial infarction associated transcript
OT  - miR-665
OT  - retinoblastoma
COIS- The authors declare that they have no competing interests.
EDAT- 2021/10/12 06:00
MHDA- 2021/10/12 06:01
PMCR- 2021/09/22
CRDT- 2021/10/11 05:57
PHST- 2020/09/24 00:00 [received]
PHST- 2021/04/28 00:00 [accepted]
PHST- 2021/10/11 05:57 [entrez]
PHST- 2021/10/12 06:00 [pubmed]
PHST- 2021/10/12 06:01 [medline]
PHST- 2021/09/22 00:00 [pmc-release]
AID - ETM-0-0-10777 [pii]
AID - 10.3892/etm.2021.10777 [doi]
PST - ppublish
SO  - Exp Ther Med. 2021 Nov;22(5):1342. doi: 10.3892/etm.2021.10777. Epub 2021 Sep 22.