PMID- 34631020
OWN - NLM
STAT- MEDLINE
DCOM- 20211021
LR  - 20230921
IS  - 2046-1402 (Electronic)
IS  - 2046-1402 (Linking)
VI  - 10
DP  - 2021
TI  - Case Report: A five-year follow up after pediatric renal transplantation using 
      flow cytometry crossmatch and HLA immunophenotyping based on DNA for screening 
      test.
PG  - 352
LID - 10.12688/f1000research.51407.1 [doi]
LID - 352
AB  - Background: There are three methods for renal replacement therapy for end stage 
      chronic kidney disease; dialysis, continuous ambulatory peritoneal dialysis, and 
      renal transplantation which is the best because of the least morbidity rate, the 
      best survival rates, the best quality of life, and the best improvement in 
      activities of daily living. In the field, flow cytometry serves a 
      well-established role in pre- and post-transplant crossmatching, and if it is 
      combined with human leukocyte antigen (HLA) immunophenotyping based on DNA, it 
      will produce a more sensitive prediction of the chronic graft rejection compared 
      to complement-dependent cytotoxicity crossmatching and can eliminate irrelevant 
      antibody (IgM). This is the first experience using this method in our hospital. 
      The survival rate at one, five and ten years has been shown to be 99%, 97% and 
      96%, respectively; therefore, we wanted to find out the five year follow up of 
      the patient. Case presentation: We evaluated a 20-year-old female with a history 
      of pediatric renal transplantation five years previously due to end stage renal 
      disease caused by bilateral parenchymatous renal disease. She had a history of 
      hypertension since December 2014 and underwent hemodialysis for three months. The 
      transplantation took place in March 2015. A kidney from her mother was 
      transplanted to recipient using end-to-side anastomoses. After five years, the 
      patient was routinely monitored at the urology clinic, with creatinine serum 
      results between 1.5 and 2 mg/dL, urea and electrolyte serum levels within normal 
      limits and she could resume normal life. Conclusions: Survival five years after 
      the procedure showed a beneficial outcome of the method used.
CI  - Copyright: (c) 2021 Dwi Meiyanto J et al.
FAU - Dwi Meiyanto, Johanes
AU  - Dwi Meiyanto J
AUID- ORCID: 0000-0001-5939-978X
AD  - Urology, Faculty of Medicine, Universitas Brawijaya - Saiful Anwar General 
      Hospital, Malang, East Java, 65145, Indonesia.
FAU - Daryanto, Besut
AU  - Daryanto B
AUID- ORCID: 0000-0002-0776-1633
AD  - Urology, Faculty of Medicine, Universitas Brawijaya - Saiful Anwar General 
      Hospital, Malang, East Java, 65145, Indonesia.
FAU - Penta Seputra, Kurnia
AU  - Penta Seputra K
AD  - Urology, Faculty of Medicine, Universitas Brawijaya - Saiful Anwar General 
      Hospital, Malang, East Java, 65145, Indonesia.
LA  - eng
PT  - Case Reports
DEP - 20210506
PL  - England
TA  - F1000Res
JT  - F1000Research
JID - 101594320
RN  - 0 (HLA Antigens)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Activities of Daily Living
MH  - Adult
MH  - DNA
MH  - Female
MH  - Flow Cytometry
MH  - Follow-Up Studies
MH  - HLA Antigens
MH  - Humans
MH  - Immunophenotyping
MH  - *Kidney Transplantation
MH  - Quality of Life
MH  - Young Adult
PMC - PMC8493425
OTO - NOTNLM
OT  - HLA based on DNA
OT  - flow cytometry crossmatch
OT  - pediatric renal transplantation
COIS- No competing interests were disclosed.
EDAT- 2021/10/12 06:00
MHDA- 2023/02/25 06:00
PMCR- 2021/05/06
CRDT- 2021/10/11 06:01
PHST- 2021/04/26 00:00 [accepted]
PHST- 2021/10/11 06:01 [entrez]
PHST- 2021/10/12 06:00 [pubmed]
PHST- 2023/02/25 06:00 [medline]
PHST- 2021/05/06 00:00 [pmc-release]
AID - 10.12688/f1000research.51407.1 [doi]
PST - epublish
SO  - F1000Res. 2021 May 6;10:352. doi: 10.12688/f1000research.51407.1. eCollection 
      2021.