PMID- 34631544
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211012
IS  - 2234-943X (Print)
IS  - 2234-943X (Electronic)
IS  - 2234-943X (Linking)
VI  - 11
DP  - 2021
TI  - DIAPH1 Promotes Laryngeal Squamous Cell Carcinoma Progression Through Cell Cycle 
      Regulation.
PG  - 716876
LID - 10.3389/fonc.2021.716876 [doi]
LID - 716876
AB  - The diaphanous related formin 1 (DIAPH1) protein is involved in the regulation of 
      dynamic cytoskeleton reorganization, which is closely related to mitosis and the 
      cell cycle. Cell cycle disorders are generally regarded as important underlying 
      causes of many cancers. In the current study, we have revealed that DIAPH1 
      expression is an independent prognostic factor for overall survival in patients 
      with laryngeal squamous cell carcinoma (LSCC) and that DIAPH1 promotes colony 
      formation, cell proliferation, and G1/S progression in LSCC cells. Additionally, 
      DIAPH1 promotes growth of AMC-HN-8 LSCC-derived tumors in vivo. In this study, 
      RNA-sequencing analysis revealed that DIAPH1 knockdown led to changes in the 
      expression of genes associated with signaling during the cell cycle. Using 
      western blot analyses, we further demonstrated that DIAPH1 knockdown resulted in 
      upregulation of p21(Waf1/Cip1), p19(Ink4d), p27(Kip1), and p16(Ink4a) and 
      downregulation of cyclinA2, cyclinD1, CDK2, CDK4, and CDK6. These results suggest 
      that DIAPH1 influences the expression of genes in several signaling pathways and 
      promotes LSCC progression by regulating the cell cycle.
CI  - Copyright (c) 2021 Yang, Huang, Guo, Wei, Zhou and Chen.
FAU - Yang, Jiechao
AU  - Yang J
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, Wuxi No. 2 People's 
      Hospital, Affiliated Wuxi Clinical College of Nangtong University, Wuxi, China.
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, Eye, Ear, Nose, and 
      Throat Hospital, Fudan University, Shanghai, China.
FAU - Huang, Qiang
AU  - Huang Q
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, Eye, Ear, Nose, and 
      Throat Hospital, Fudan University, Shanghai, China.
FAU - Guo, Yang
AU  - Guo Y
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, Eye, Ear, Nose, and 
      Throat Hospital, Fudan University, Shanghai, China.
FAU - Wei, Zheqiang
AU  - Wei Z
AD  - Department of Pathology, Affiliated Hospital of Jiangnan University, Wuxi, China.
FAU - Zhou, Liang
AU  - Zhou L
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, Eye, Ear, Nose, and 
      Throat Hospital, Fudan University, Shanghai, China.
FAU - Chen, Hui
AU  - Chen H
AD  - Department of Otorhinolaryngology-Head and Neck Surgery, Eye, Ear, Nose, and 
      Throat Hospital, Fudan University, Shanghai, China.
LA  - eng
PT  - Journal Article
DEP - 20210922
PL  - Switzerland
TA  - Front Oncol
JT  - Frontiers in oncology
JID - 101568867
PMC - PMC8494199
OTO - NOTNLM
OT  - DIAPH1
OT  - G1/S progression
OT  - LSCC
OT  - cell cycle regulation
OT  - mitosis
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2021/10/12 06:00
MHDA- 2021/10/12 06:01
PMCR- 2021/01/01
CRDT- 2021/10/11 06:08
PHST- 2021/05/29 00:00 [received]
PHST- 2021/09/03 00:00 [accepted]
PHST- 2021/10/11 06:08 [entrez]
PHST- 2021/10/12 06:00 [pubmed]
PHST- 2021/10/12 06:01 [medline]
PHST- 2021/01/01 00:00 [pmc-release]
AID - 10.3389/fonc.2021.716876 [doi]
PST - epublish
SO  - Front Oncol. 2021 Sep 22;11:716876. doi: 10.3389/fonc.2021.716876. eCollection 
      2021.