PMID- 34634160
OWN - NLM
STAT- MEDLINE
DCOM- 20220718
LR  - 20220720
IS  - 1600-0714 (Electronic)
IS  - 0904-2512 (Linking)
VI  - 51
IP  - 6
DP  - 2022 Jul
TI  - HAGLROS promotes cell proliferation and angiogenesis and inhibits apoptosis by 
      activating multiple signaling pathways in LSCC cells.
PG  - 510-519
LID - 10.1111/jop.13249 [doi]
AB  - BACKGROUND: HAGLROS is a long noncoding RNA involving in the development of a 
      variety of cancers, but its mechanism of action in laryngeal squamous cell 
      carcinomas (LSCC) is still unclear. We aim to unveil the effect and mechanism of 
      HAGLROS on LSCC. METHODS: The expression of HAGLROS in LSCC patients' tissues, 
      serum, and LSCC cell lines was quantified by quantitative real-time PCR. AMC-HN-8 
      and SNU-46 cells were transfected with the overexpression plasmid of HAGLROS and 
      shHAGLROS, and the functional assay (colony formation assays, flow cytometry, and 
      tube formation) was performed. Western blot was used to determine the expressions 
      of vascular endothelial growth factor (VEGF), proliferating cell nuclear antigen 
      (PCNA), P27 and cleaved caspase-3, as well as phosphorylated-c-Jun-N-terminal 
      kinase (p-JNK), JNK, phosphorylated-extracellular signal-regulated kinase 1/2 
      (p-Erk1/2), Erk1/2, phosphorylated-protein kinase B (p-AKT) and AKT. RESULTS: 
      HAGLROS was highly expressed in LSCC tissues and cells, and it was correlated to 
      lymph node, tumor depth, and clinical stage of LSCC patients. The proliferation 
      ability of LSCC cells was higher than that of HuLa-PC cells. Meanwhile, HAGLROS 
      overexpression promoted the abilities of proliferation and angiogenesis and 
      reduced apoptosis, whereas silencing of HAGLROS exerted the opposite effects in 
      LSCC cell lines. Moreover, overexpressed HAGLROS upregulated the expressions of 
      VEGF and PCNA yet downregulated the expressions of P27 and cleaved caspase-3 by 
      activating Erk1/2 and AKT or JNK signaling pathways in different LSCC cell lines. 
      CONCLUSION: Overexpressed HAGLROS promoted the proliferation and angiogenesis yet 
      inhibited apoptosis of LSCC cells by activating Erk1/2 and AKT or JNK signaling 
      pathways.
CI  - (c) 2021 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd.
FAU - Ma, Yunxia
AU  - Ma Y
AD  - Department of Otorhinolaryngology Head and Neck Surgery, The First Affiliated 
      Hospital of Anhui Medical University, Hefei, China.
FAU - Zhang, Hui
AU  - Zhang H
AD  - Department of Radiology, The First Affiliated Hospital of Anhui Medical 
      University, Hefei, China.
FAU - Li, Xiaohong
AU  - Li X
AD  - Department of Otorhinolaryngology Head and Neck Surgery, The First Affiliated 
      Hospital of Anhui Medical University, Hefei, China.
FAU - Liu, Yehai
AU  - Liu Y
AUID- ORCID: 0000-0001-6946-7109
AD  - Department of Otorhinolaryngology Head and Neck Surgery, The First Affiliated 
      Hospital of Anhui Medical University, Hefei, China.
LA  - eng
PT  - Journal Article
DEP - 20211025
PL  - Denmark
TA  - J Oral Pathol Med
JT  - Journal of oral pathology & medicine : official publication of the International 
      Association of Oral Pathologists and the American Academy of Oral Pathology
JID - 8911934
RN  - 0 (Proliferating Cell Nuclear Antigen)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (Vascular Endothelial Growth Factor A)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 3.4.22.- (Caspase 3)
SB  - IM
MH  - Apoptosis/genetics
MH  - Caspase 3/metabolism
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - Gene Expression Regulation, Neoplastic/genetics
MH  - Humans
MH  - *Laryngeal Neoplasms/metabolism
MH  - Proliferating Cell Nuclear Antigen/genetics
MH  - Proto-Oncogene Proteins c-akt/genetics/metabolism
MH  - RNA, Long Noncoding
MH  - Signal Transduction/genetics
MH  - Squamous Cell Carcinoma of Head and Neck/genetics
MH  - Vascular Endothelial Growth Factor A/genetics/metabolism
OTO - NOTNLM
OT  - HAGLROS
OT  - angiogenesis
OT  - apoptosis
OT  - laryngeal squamous cell carcinomas
OT  - proliferation
EDAT- 2021/10/12 06:00
MHDA- 2022/07/19 06:00
CRDT- 2021/10/11 17:24
PHST- 2021/09/07 00:00 [revised]
PHST- 2021/05/13 00:00 [received]
PHST- 2021/10/08 00:00 [accepted]
PHST- 2021/10/12 06:00 [pubmed]
PHST- 2022/07/19 06:00 [medline]
PHST- 2021/10/11 17:24 [entrez]
AID - 10.1111/jop.13249 [doi]
PST - ppublish
SO  - J Oral Pathol Med. 2022 Jul;51(6):510-519. doi: 10.1111/jop.13249. Epub 2021 Oct 
      25.