PMID- 34635260
OWN - NLM
STAT- MEDLINE
DCOM- 20211013
LR  - 20211013
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 236
DP  - 2022 Jan 1
TI  - Aptamer-functionalized metal-organic framework-coated nanofibers with 
      multi-affinity sites for highly sensitive, selective recognition of ultra-trace 
      microcystin-LR.
PG  - 122880
LID - S0039-9140(21)00801-8 [pii]
LID - 10.1016/j.talanta.2021.122880 [doi]
AB  - A novel aptamer-functionalized metal-organic framework nanofibrous composite 
      (viz. PAN/UiO@UiO2-N(3)-aptamer) with a high aptamer coverage density was 
      proposed based on the electrospinning and seeded growth method, and used for 
      specific affinity recognition of trace Microcystin-LR (MC-LR). Heterobifunctional 
      ligand was used to modify the metal-organic framework nanoparticles (MOF NPs) 
      surface, which could passivate the MOF surface with respect to unmodified DNA, 
      followed by coupling massive aptamers on MOF of the solid-phase microextraction 
      (SPME) fiber using click chemistry. Characterizations including morphology, 
      spectra analysis, mechanical stability, binding capacity and specificity were 
      fulfilled. Applied to the analysis of MC-LR, the good selective and sensitive 
      recognition were obtained with the detection limit as low as 0.003 ng/mL, which 
      was better than most non-specific SPME or solid-phase extraction (SPE) protocols. 
      The stability and reproducibility were acceptable, and the intra-day, inter-day 
      and column-to-column relative standard deviations (RSDs) for the recovery of 
      MC-LR were gained in the range from 2.5% to 14.3%, respectively. Satisfactory 
      recoveries of MC-LR in environmental water samples were measured as 96.3 +/- 4.7% - 
      98.9 +/- 2.7% (n = 3) in tap water, 94.4 +/- 2.5% - 96.1 +/- 3.5% (n = 3) in pond 
      water, and 97.0 +/- 2.1% - 97.9 +/- 3.1% (n = 3) in river water, respectively. This 
      work demonstrated that the electrospun nanofibrous composite with massive 
      aptamers would be a better alternative for ultra-trace MC-LR detection with good 
      selectivity, matrix-resistance ability and high resolution.
CI  - Copyright (c) 2021 Elsevier B.V. All rights reserved.
FAU - Xu, Zhiqun
AU  - Xu Z
AD  - Institute of Food Safety and Environment Monitoring, Fuzhou University, Fuzhou, 
      350108, People's Republic of China.
FAU - Zhang, Zhexiang
AU  - Zhang Z
AD  - Institute of Food Safety and Environment Monitoring, Fuzhou University, Fuzhou, 
      350108, People's Republic of China.
FAU - She, Zongkang
AU  - She Z
AD  - Institute of Food Safety and Environment Monitoring, Fuzhou University, Fuzhou, 
      350108, People's Republic of China.
FAU - Lin, Chenchen
AU  - Lin C
AD  - Institute of Food Safety and Environment Monitoring, Fuzhou University, Fuzhou, 
      350108, People's Republic of China.
FAU - Lin, Xucong
AU  - Lin X
AD  - Institute of Food Safety and Environment Monitoring, Fuzhou University, Fuzhou, 
      350108, People's Republic of China; Engineering Technology Research Center on 
      Reagent and Instrument for Rapid Detection of Product Quality and Food Safety, 
      Fujian, Fuzhou, 350108, People's Republic of China. Electronic address: 
      xulin@fzu.edu.cn.
FAU - Xie, Zenghong
AU  - Xie Z
AD  - Institute of Food Safety and Environment Monitoring, Fuzhou University, Fuzhou, 
      350108, People's Republic of China; Engineering Technology Research Center on 
      Reagent and Instrument for Rapid Detection of Product Quality and Food Safety, 
      Fujian, Fuzhou, 350108, People's Republic of China.
LA  - eng
PT  - Journal Article
DEP - 20210911
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
RN  - 0 (Aptamers, Nucleotide)
RN  - 0 (Marine Toxins)
RN  - 0 (Metal-Organic Frameworks)
RN  - 0 (Microcystins)
RN  - EQ8332842Y (cyanoginosin LR)
SB  - IM
MH  - *Aptamers, Nucleotide
MH  - Marine Toxins
MH  - *Metal-Organic Frameworks
MH  - Microcystins
MH  - *Nanofibers
MH  - Reproducibility of Results
OTO - NOTNLM
OT  - Aptamer
OT  - Click chemistry
OT  - Electrospinning
OT  - Microcystin-LR
OT  - Selective extraction
EDAT- 2021/10/13 06:00
MHDA- 2021/10/14 06:00
CRDT- 2021/10/12 05:42
PHST- 2021/07/15 00:00 [received]
PHST- 2021/09/08 00:00 [revised]
PHST- 2021/09/10 00:00 [accepted]
PHST- 2021/10/12 05:42 [entrez]
PHST- 2021/10/13 06:00 [pubmed]
PHST- 2021/10/14 06:00 [medline]
AID - S0039-9140(21)00801-8 [pii]
AID - 10.1016/j.talanta.2021.122880 [doi]
PST - ppublish
SO  - Talanta. 2022 Jan 1;236:122880. doi: 10.1016/j.talanta.2021.122880. Epub 2021 Sep 
      11.