PMID- 34635987
OWN - NLM
STAT- MEDLINE
DCOM- 20211223
LR  - 20221013
IS  - 1995-820X (Electronic)
IS  - 1674-0769 (Print)
IS  - 1995-820X (Linking)
VI  - 36
IP  - 6
DP  - 2021 Dec
TI  - The Novel PRRSV Strain HBap4-2018 with a Unique Recombinant Pattern Is Highly 
      Pathogenic to Piglets.
PG  - 1611-1625
LID - 10.1007/s12250-021-00453-0 [doi]
AB  - Currently, various porcine reproductive and respiratory syndrome virus (PRRSV) 
      variants emerged worldwide with different genetic characteristics and 
      pathogenicity, increasing the difficulty of PRRS control. In this study, a PRRSV 
      strain named HBap4-2018 was isolated from swine herds suffering severe 
      respiratory disease with high morbidity in Hebei Province of China in 2018. The 
      genome of HBap4-2018 is 15,003 nucleotides in length, and compared with 
      NADC30-like PRRSV, nsp2 of HBap4-2018 has an additional continuous deletion of 
      five amino acids. Phylogenetic analysis based on complete genome and ORF5 showed 
      that HBap4-2018 belonged to lineage 8 of PRRSV-2, which was characterized by 
      highly variable genome. However, HBap4-2018 was classified into lineage 1 based 
      on phylogenetic analysis of nsp2, sharing higher amino acid homology 
      (85.3%-85.5%) with NADC30-like PRRSV. Further analysis suggested that HBap4-2018 
      was a novel natural recombinant PRRSV with three recombinant fragments in the 
      genome, of which highly pathogenic PRRSV (HP-PRRSV) served as the major parental 
      strains, while NADC30-like PRRSV served as the minor parental strains. Five 
      recombination break points were identified in nsp2, nsp3, nsp5, nsp9 and ORF6, 
      respectively, presenting a novel recombinant pattern in the genome. Piglets 
      inoculated with HBap4-2018 presented typical clinical signs with a mortality rate 
      of 60%. High levels of viremia and obvious macroscopic and histopathological 
      lesions in the lungs were observed, revealing the high pathogenicity of 
      HBap4-2018 in piglets.
CI  - (c) 2021. Wuhan Institute of Virology, CAS.
FAU - Chen, Pengfei
AU  - Chen P
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai, 200241, China.
AD  - Jiangsu Co-Innovation Center for Prevention and Control of Important Animal 
      Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, China.
FAU - Tan, Xiangmei
AU  - Tan X
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai, 200241, China.
FAU - Lao, Mengqin
AU  - Lao M
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai, 200241, China.
FAU - Wu, Xia
AU  - Wu X
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai, 200241, China.
FAU - Zhao, Xiongwei
AU  - Zhao X
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai, 200241, China.
FAU - Zhou, Shuting
AU  - Zhou S
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai, 200241, China.
AD  - Shanghai Key Laboratory of Veterinary Biotechnology, School of Agriculture and 
      Biology, Shanghai Jiao Tong University, Shanghai, 200240, China.
FAU - Yu, Jiarong
AU  - Yu J
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai, 200241, China.
FAU - Zhu, Junrui
AU  - Zhu J
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai, 200241, China.
FAU - Yu, Lingxue
AU  - Yu L
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai, 200241, China.
FAU - Tong, Wu
AU  - Tong W
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai, 200241, China.
FAU - Gao, Fei
AU  - Gao F
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai, 200241, China.
FAU - Yu, Hai
AU  - Yu H
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai, 200241, China.
FAU - Liu, Changlong
AU  - Liu C
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai, 200241, China.
FAU - Jiang, Yifeng
AU  - Jiang Y
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai, 200241, China.
FAU - Tong, Guangzhi
AU  - Tong G
AUID- ORCID: 0000-0001-7048-0837
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai, 200241, China. 
      gztong@shvri.ac.cn.
AD  - Jiangsu Co-Innovation Center for Prevention and Control of Important Animal 
      Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, China. 
      gztong@shvri.ac.cn.
FAU - Zhou, Yanjun
AU  - Zhou Y
AUID- ORCID: 0000-0002-2524-0831
AD  - Department of Swine Infectious Diseases, Shanghai Veterinary Research Institute, 
      Chinese Academy of Agricultural Sciences, Shanghai, 200241, China. 
      yjzhou@shvri.ac.cn.
AD  - Jiangsu Co-Innovation Center for Prevention and Control of Important Animal 
      Infectious Diseases and Zoonoses, Yangzhou University, Yangzhou, 225009, China. 
      yjzhou@shvri.ac.cn.
LA  - eng
PT  - Journal Article
DEP - 20211012
PL  - Netherlands
TA  - Virol Sin
JT  - Virologica Sinica
JID - 101514185
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - China
MH  - Genetic Variation
MH  - Genome, Viral
MH  - Phylogeny
MH  - *Porcine Reproductive and Respiratory Syndrome
MH  - *Porcine respiratory and reproductive syndrome virus/genetics
MH  - Swine
PMC - PMC8692517
OTO - NOTNLM
OT  - Genetic variation
OT  - Nsp2
OT  - Pathogenicity
OT  - Porcine reproductive and respiratory syndrome virus (PRRSV)
OT  - Recombination
COIS- The authors declare that they have no conflict of interest.
EDAT- 2021/10/13 06:00
MHDA- 2021/12/24 06:00
PMCR- 2022/10/12
CRDT- 2021/10/12 06:50
PHST- 2021/06/15 00:00 [received]
PHST- 2021/08/18 00:00 [accepted]
PHST- 2021/10/13 06:00 [pubmed]
PHST- 2021/12/24 06:00 [medline]
PHST- 2021/10/12 06:50 [entrez]
PHST- 2022/10/12 00:00 [pmc-release]
AID - 10.1007/s12250-021-00453-0 [pii]
AID - 453 [pii]
AID - 10.1007/s12250-021-00453-0 [doi]
PST - ppublish
SO  - Virol Sin. 2021 Dec;36(6):1611-1625. doi: 10.1007/s12250-021-00453-0. Epub 2021 
      Oct 12.