PMID- 34636141
OWN - NLM
STAT- MEDLINE
DCOM- 20220204
LR  - 20220204
IS  - 1751-553X (Electronic)
IS  - 1751-5521 (Linking)
VI  - 44
IP  - 1
DP  - 2022 Feb
TI  - The success rate of interphase fluorescence in situ hybridization in plasma cell 
      disorders can be improved using unconventional sources of plasma cells.
PG  - 157-162
LID - 10.1111/ijlh.13731 [doi]
AB  - BACKGROUND: Immunomagnetic cell sorting (IMCS) is a preferred technique for the 
      enrichment of plasma cells (PC) before fluorescence in situ hybridization (FISH). 
      Here, we share our real-world experience regarding the success rate of IMCS, its 
      limitations, and the utility of alternate sources to obtain a successful FISH in 
      various PC disorders. MATERIALS AND METHODS: A retrospective analysis was 
      performed in patients with a PC neoplasm, who underwent bone marrow (BM) 
      examination, and FISH testing over 30 months. In all cases with an unsuccessful 
      IMCS, an attempt was made to identify the cause of failure. RESULTS: 
      Immunomagnetic cell sorting of PCs was successful in 395/450 cases (87.8%; 77/98 
      cases (78.6%) with <10% PCs and 318/352 (90.3%) with >/=10% PCs in BM aspirate; 
      P = .003). Among cases with unsuccessful IMCS (<10% PCs; n = 21 and >/=10% PCs; 
      n = 34), an alternate source could be used successfully in 34 (62%) patients and 
      includes air-dried trephine biopsy imprint smears (n = 28) with aggregates or 
      sheets of PCs, fine-needle aspiration smears/biopsy from plasmacytoma (n = 5), 
      and ascitic fluid (n = 1). 284/395 (71.9%) patients with successful IMCS and all 
      34 cases with an alternate source of PCs showed at least one cytogenetic 
      abnormality on four-probe FISH. CONCLUSION: Variations in the sample quality 
      together with significant variation in the number of PCs between BM aspirate and 
      the trephine biopsy imprint smears/biopsy reduce the success rate of IMCS in a 
      real-world scenario and necessitate utilization of patient-specific alternate 
      sources of PCs like a trephine biopsy imprint or cytology smears from 
      extramedullary sources for successful FISH testing in PC neoplasms.
CI  - (c) 2021 John Wiley & Sons Ltd.
FAU - Panakkal, Vandana
AU  - Panakkal V
AD  - Department of Hematology, Post Graduate Institute of Medical Education and 
      Research, Chandigarh, India.
FAU - Rana, Sonia
AU  - Rana S
AD  - Department of Hematology, Post Graduate Institute of Medical Education and 
      Research, Chandigarh, India.
FAU - Rathore, Shailja
AU  - Rathore S
AD  - Department of Hematology, Post Graduate Institute of Medical Education and 
      Research, Chandigarh, India.
FAU - Anshu, Anshu
AU  - Anshu A
AD  - Department of Hematology, Post Graduate Institute of Medical Education and 
      Research, Chandigarh, India.
FAU - Balakrishnan, Anand
AU  - Balakrishnan A
AD  - Department of Hematology, Post Graduate Institute of Medical Education and 
      Research, Chandigarh, India.
FAU - Singh, Charanpreet
AU  - Singh C
AD  - Adult Clinical Hematology and Bone Marrow Transplantation Unit, Department of 
      Internal Medicine, Post Graduate Institute of Medical Education and Research, 
      Chandigarh, India.
FAU - Jandial, Aditya
AU  - Jandial A
AD  - Adult Clinical Hematology and Bone Marrow Transplantation Unit, Department of 
      Internal Medicine, Post Graduate Institute of Medical Education and Research, 
      Chandigarh, India.
FAU - Sachdeva, Man Updesh Singh
AU  - Sachdeva MUS
AD  - Department of Hematology, Post Graduate Institute of Medical Education and 
      Research, Chandigarh, India.
FAU - Varma, Neelam
AU  - Varma N
AUID- ORCID: 0000-0002-1170-5976
AD  - Department of Hematology, Post Graduate Institute of Medical Education and 
      Research, Chandigarh, India.
FAU - Lad, Deepesh
AU  - Lad D
AD  - Adult Clinical Hematology and Bone Marrow Transplantation Unit, Department of 
      Internal Medicine, Post Graduate Institute of Medical Education and Research, 
      Chandigarh, India.
FAU - Malhotra, Pankaj
AU  - Malhotra P
AD  - Adult Clinical Hematology and Bone Marrow Transplantation Unit, Department of 
      Internal Medicine, Post Graduate Institute of Medical Education and Research, 
      Chandigarh, India.
FAU - Sreedharanunni, Sreejesh
AU  - Sreedharanunni S
AUID- ORCID: 0000-0003-2626-4154
AD  - Department of Hematology, Post Graduate Institute of Medical Education and 
      Research, Chandigarh, India.
LA  - eng
GR  - 71/2-EDU-16/657/Postgraduate Institute of Medical Education and Research, 
      Chandigarh/
PT  - Journal Article
DEP - 20211011
PL  - England
TA  - Int J Lab Hematol
JT  - International journal of laboratory hematology
JID - 101300213
RN  - 0 (Biomarkers, Tumor)
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Biomarkers, Tumor
MH  - Bone Marrow/pathology
MH  - Chromosome Aberrations
MH  - Cytological Techniques
MH  - Diagnosis, Differential
MH  - Disease Management
MH  - Disease Susceptibility
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods/standards
MH  - Interphase/*genetics
MH  - Male
MH  - Middle Aged
MH  - Neoplasm Grading
MH  - Neoplasm Staging
MH  - Neoplasms, Plasma Cell/*diagnosis/*genetics
MH  - Plasma Cells/*metabolism/*pathology
MH  - Retrospective Studies
OTO - NOTNLM
OT  - bone marrow biopsy imprints
OT  - cytogenetics
OT  - fine-needle aspiration cytology
OT  - immunomagnetic cell sorting
OT  - multiple myeloma
OT  - plasma cell enrichment
EDAT- 2021/10/13 06:00
MHDA- 2022/02/05 06:00
CRDT- 2021/10/12 07:01
PHST- 2021/08/28 00:00 [revised]
PHST- 2021/06/15 00:00 [received]
PHST- 2021/09/28 00:00 [accepted]
PHST- 2021/10/13 06:00 [pubmed]
PHST- 2022/02/05 06:00 [medline]
PHST- 2021/10/12 07:01 [entrez]
AID - 10.1111/ijlh.13731 [doi]
PST - ppublish
SO  - Int J Lab Hematol. 2022 Feb;44(1):157-162. doi: 10.1111/ijlh.13731. Epub 2021 Oct 
      11.