PMID- 34636628
OWN - NLM
STAT- MEDLINE
DCOM- 20220406
LR  - 20240214
IS  - 1947-6043 (Electronic)
IS  - 1947-6035 (Print)
IS  - 1947-6035 (Linking)
VI  - 13
IP  - 2_suppl
DP  - 2021 Dec
TI  - Migrating Progenitor Cells Derived From Injured Cartilage Surface Respond to 
      Damage-Associated Molecular Patterns.
PG  - 755S-765S
LID - 10.1177/19476035211049559 [doi]
AB  - OBJECTIVE: To delineate the response of migrating chondrogenic progenitor cells 
      (CPCs) that arose from the surface of mechanically injured articular cartilage to 
      proinflammatory damage-associated-molecular-patterns (DAMPs). DESIGN: Bovine CPCs 
      and non-CPC chondrocytes isolated from either impacted or scratched articular 
      cartilage were studied. Those 2 types of cells were treated with mitochondrial 
      DAMPs (MTDs; 10 nM fMLF and 10 microg/mL CpG DNA), or 10 nM HMGB1, or 10 ng/mL IL-1b 
      for 24 hours. At the end of experiments, conditioned media and cell lysates were 
      collected for analysis of expression levels of matrix metalloproteinases (MMPs), 
      chemokines, and cytokines that are associated with cartilage degeneration with 
      Western blotting and quantitative polymerase chain reaction. The difference of 
      expression levels was compared by Welch's t-test. RESULTS: Our data indicated 
      that HMGB1 and MTDs remarkably upregulated pro-MMP-13 expression in CPCs. 
      Compared with non-CPCs, CPCs expressed significantly more baseline mRNAs of 
      MMP-13, CXCL12, and IL-6. MTDs greatly increased the expression of MMP-13 and 
      IL-6 in CPCs by over 100-fold (P < 0.001). MTDs also significantly increased IL-8 
      expression in CPCs to a similar extent (P < 0.001). However, when IL-1b was 
      present, CPCs expressed less MMP-3 and active MMP-13 proteins as well as less 
      CCL2 and IL-6 than did non-CPCs. CONCLUSIONS: We concluded that CPCs were more 
      sensitive than non-CPCs in response to DAMPs, especially MTDs. The 
      proinflammatory nature of CPCs implied their critical role in the early phase of 
      posttraumatic osteoarthritis development.
FAU - Ding, Lei
AU  - Ding L
AUID- ORCID: 0000-0001-6993-8273
AD  - Department of Basic Medical Sciences, Wuxi College of Medicine, Jiangnan 
      University, Wuxi, Jiangsu, China.
AD  - Department of Orthopaedics and Rehabilitation, University of Iowa Hospitals and 
      Clinics, Iowa City, IA, USA.
FAU - Zhou, Cheng
AU  - Zhou C
AD  - Department of Orthopaedics and Rehabilitation, University of Iowa Hospitals and 
      Clinics, Iowa City, IA, USA.
FAU - Zheng, Hongjun
AU  - Zheng H
AD  - Department of Orthopaedics and Rehabilitation, University of Iowa Hospitals and 
      Clinics, Iowa City, IA, USA.
FAU - Wang, Quanming
AU  - Wang Q
AD  - Department of Orthopaedic Surgery, Affiliated Hospital, Jiangnan University, 
      Wuxi, Jiangsu, China.
FAU - Song, Haiyan
AU  - Song H
AD  - Department of Endocrinology and Metabolism, The Second Affiliated Hospital of 
      Harbin Medical University, Harbin, China.
FAU - Buckwalter, Joseph A
AU  - Buckwalter JA
AD  - Department of Orthopaedics and Rehabilitation, University of Iowa Hospitals and 
      Clinics, Iowa City, IA, USA.
AD  - Veterans Affairs Medical Center, Iowa City, IA, USA.
FAU - Martin, James A
AU  - Martin JA
AD  - Department of Orthopaedics and Rehabilitation, University of Iowa Hospitals and 
      Clinics, Iowa City, IA, USA.
LA  - eng
GR  - P50 AR055533/AR/NIAMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20211012
PL  - United States
TA  - Cartilage
JT  - Cartilage
JID - 101518378
SB  - IM
MH  - Animals
MH  - *Cartilage, Articular/metabolism
MH  - Cattle
MH  - Chondrocytes/metabolism
MH  - Chondrogenesis
MH  - *Osteoarthritis/metabolism
MH  - Stem Cells/metabolism
PMC - PMC8804768
OTO - NOTNLM
OT  - cartilage degeneration
OT  - chondrogenic progenitor cells
OT  - damage-associated molecular patterns
OT  - inflammation
OT  - posttraumatic osteoarthritis
COIS- Declaration of Conflicting Interests: The author(s) declared no potential 
      conflicts of interest with respect to the research, authorship, and/or 
      publication of this article.
EDAT- 2021/10/13 06:00
MHDA- 2022/04/07 06:00
PMCR- 2022/06/01
CRDT- 2021/10/12 12:20
PHST- 2021/10/13 06:00 [pubmed]
PHST- 2022/04/07 06:00 [medline]
PHST- 2021/10/12 12:20 [entrez]
PHST- 2022/06/01 00:00 [pmc-release]
AID - 10.1177_19476035211049559 [pii]
AID - 10.1177/19476035211049559 [doi]
PST - ppublish
SO  - Cartilage. 2021 Dec;13(2_suppl):755S-765S. doi: 10.1177/19476035211049559. Epub 
      2021 Oct 12.