PMID- 34637014
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240403
IS  - 1931-7573 (Print)
IS  - 1556-276X (Electronic)
IS  - 1556-276X (Linking)
VI  - 16
IP  - 1
DP  - 2021 Oct 12
TI  - Black Phosphorus Nanoparticles Promote Osteogenic Differentiation of EMSCs 
      Through Upregulated TG2 Expression.
PG  - 154
LID - 10.1186/s11671-021-03610-2 [doi]
LID - 154
AB  - At bio-safe concentrations, black phosphorus nanoparticles activated TG2, and 
      promote the expression of ECM, which further promoted osteogenic differentiation 
      of EMSCs. From these results, we can conclude that black phosphorus nanoparticles 
      are suitable as biological factors in bone tissue engineering. Black phosphorus 
      nanoparticles (BPs) present excellent biocompatibility and good biodegradability, 
      which have been rigorously studied and proven. However, its utilization in bone 
      tissue engineering fields is still in its infancy. Thus, the main purpose of the 
      present study was to investigate the effects of BPs on osteogenic differentiation 
      of ectodermal mesenchymal stem cell (EMSC) in vitro. Biocompatible BPs with high 
      yield were prepared with a simple and efficient ultrasonication technique. EMSCs 
      were isolated from adult rat nasal respiratory mucosa. Then, we treated EMSCs 
      with BPs at different concentrations in vitro and examined the effect of BPs on 
      osteogenic differentiation of EMSCs. In addition, inhibitor of transglutaminase 2 
      (TG2) and western blot were used to clarify the mechanism of the promoting effect 
      of BPs on osteogenesis. Our results indicated that BPs could significantly 
      enhance osteogenic differentiation of EMSCs in vitro. Nevertheless, BPs had no 
      effect on EMSCs proliferation. Mechanistically, BPs promoted osteogenesis 
      differentiation of EMSCs through upregulating TG2 expression. These results 
      highlight the advantage of using chemical materials for novel engineering 
      strategies of these highly promising small molecules for bone-tissue 
      regeneration.
CI  - (c) 2021. The Author(s).
FAU - Lu, Naiyan
AU  - Lu N
AD  - State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 
      214122, Jiangsu Province, People's Republic of China.
FAU - Wang, Xinhe
AU  - Wang X
AD  - State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 
      214122, Jiangsu Province, People's Republic of China.
FAU - Shi, Wentao
AU  - Shi W
AD  - School of Medicine, Jiangnan University, Wuxi, 214122, Jiangsu Province, People's 
      Republic of China.
FAU - Bian, Lu
AU  - Bian L
AD  - School of Medicine, Jiangnan University, Wuxi, 214122, Jiangsu Province, People's 
      Republic of China.
FAU - Zhang, Xuan
AU  - Zhang X
AD  - State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 
      214122, Jiangsu Province, People's Republic of China.
FAU - Yang, Guofeng
AU  - Yang G
AD  - State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 
      214122, Jiangsu Province, People's Republic of China.
FAU - Tang, Xue
AU  - Tang X
AD  - State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 
      214122, Jiangsu Province, People's Republic of China.
FAU - Wang, Jun
AU  - Wang J
AD  - State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, 
      214122, Jiangsu Province, People's Republic of China.
FAU - Zou, Yin
AU  - Zou Y
AUID- ORCID: 0000-0001-9778-2819
AD  - The Affiliated Wuxi Children's Hospital of Nanjing Medical University, Wuxi, 
      214023, Jiangsu Province, People's Republic of China. zouyin21@njmu.edu.cn.
FAU - Weng, Yuyan
AU  - Weng Y
AD  - Center for Soft Condensed Matter Physics and Interdisciplinary Research, Soochow 
      University, Suzhou, 215006, People's Republic of China. wengyuyan@suda.edu.cn.
LA  - eng
GR  - 31871865/National Natural Science Foundation of China/
PT  - Journal Article
DEP - 20211012
PL  - United States
TA  - Nanoscale Res Lett
JT  - Nanoscale research letters
JID - 101279750
PMC - PMC8511187
OTO - NOTNLM
OT  - Black phosphorus nanoparticles
OT  - EMSCs
OT  - Osteogenic differentiation
OT  - Transglutaminase 2
COIS- The authors declare that they have no competing interests.
EDAT- 2021/10/13 06:00
MHDA- 2021/10/13 06:01
PMCR- 2021/10/12
CRDT- 2021/10/12 13:00
PHST- 2021/07/22 00:00 [received]
PHST- 2021/09/28 00:00 [accepted]
PHST- 2021/10/12 13:00 [entrez]
PHST- 2021/10/13 06:00 [pubmed]
PHST- 2021/10/13 06:01 [medline]
PHST- 2021/10/12 00:00 [pmc-release]
AID - 10.1186/s11671-021-03610-2 [pii]
AID - 3610 [pii]
AID - 10.1186/s11671-021-03610-2 [doi]
PST - epublish
SO  - Nanoscale Res Lett. 2021 Oct 12;16(1):154. doi: 10.1186/s11671-021-03610-2.