PMID- 34637606
OWN - NLM
STAT- MEDLINE
DCOM- 20220404
LR  - 20220405
IS  - 2050-4527 (Electronic)
IS  - 2050-4527 (Linking)
VI  - 10
IP  - 1
DP  - 2022 Jan
TI  - Adult allergic rhinitis sufferers have unique nasal mucosal and peripheral blood 
      immune gene expression profiles: A case-control study.
PG  - 78-92
LID - 10.1002/iid3.545 [doi]
AB  - BACKGROUND: Allergic rhinitis (AR) is a complex disease involving both mucosal 
      and systemic immune compartments. Greater understanding of the immune networks 
      underpinning AR pathophysiology may assist with further refining disease-specific 
      biomarkers. OBJECTIVE: To compare immune gene expression profiles in nasal mucosa 
      and peripheral blood samples between adults with AR and controls without AR. 
      METHODS: This cross-sectional study included 45 adults with moderate-severe and 
      persistent AR (37.6 +/- 12.8 years; mean +/- SD) and 24 adults without AR 
      (36.6 +/- 10.2). Gene expression analysis was performed using the NanoString 
      nCounter PanCancer Immune profiling panel (n = 730 immune genes) in combination 
      with the panel plus probe set (n = 30 allergy-related genes) with purified RNA 
      from peripheral blood and cell lysates prepared from combined nasal lavage and 
      nasal brushing. RESULTS: One hundred and thirteen genes were significantly 
      differentially expressed in peripheral blood samples between groups (p < .05). In 
      contrast, 14 genes were differentially expressed in nasal lysate samples between 
      groups (p < .05). Upregulation of allergy-related genes in nasal mucosa samples 
      in the AR group was observed. Namely, chemokines CCL17 and CCL26 are involved in 
      the chemotaxis of key effector cells and TPSAB1 encodes tryptase, an inflammatory 
      mediator released from activated mast cells and basophils. Six differentially 
      expressed genes (DEGs) were in common between the nasal mucosa and blood samples. 
      In addition, counts of specific DEGs in nasal mucosa samples were positively 
      correlated with eosinophil and dust mite-specific immunoglobulin E (IgE) counts 
      in blood. CONCLUSIONS AND CLINICAL RELEVANCE: Distinct gene expression profiles 
      in blood and nasal mucosa samples were observed between AR sufferers and 
      controls. The results of this study also provide evidence for a close interaction 
      between the local site and systemic immunity. The genes identified in this study 
      contribute to the current knowledge of AR pathophysiology and may serve as 
      biomarkers to evaluate the effectiveness of treatment regimens, or as targets for 
      drug discovery.
CI  - (c) 2021 The Authors. Immunity, Inflammation and Disease published by John Wiley & 
      Sons Ltd.
FAU - Watts, Annabelle M
AU  - Watts AM
AD  - Menzies Health Institute of Queensland, Griffith University, Southport, 
      Queensland, Australia.
AD  - School of Medical Science, Griffith University, Southport, Queensland, Australia.
FAU - West, Nicholas P
AU  - West NP
AD  - Menzies Health Institute of Queensland, Griffith University, Southport, 
      Queensland, Australia.
AD  - School of Medical Science, Griffith University, Southport, Queensland, Australia.
FAU - Smith, Peter K
AU  - Smith PK
AD  - School of Medicine, Griffith University, Southport, Queensland, Australia.
AD  - Queensland Allergy Services Clinic, Southport, Queensland, Australia.
FAU - Cripps, Allan W
AU  - Cripps AW
AUID- ORCID: 0000-0002-8755-932X
AD  - Menzies Health Institute of Queensland, Griffith University, Southport, 
      Queensland, Australia.
AD  - School of Medicine, Griffith University, Southport, Queensland, Australia.
FAU - Cox, Amanda J
AU  - Cox AJ
AUID- ORCID: 0000-0003-3684-0952
AD  - Menzies Health Institute of Queensland, Griffith University, Southport, 
      Queensland, Australia.
AD  - School of Medical Science, Griffith University, Southport, Queensland, Australia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20211012
PL  - England
TA  - Immun Inflamm Dis
JT  - Immunity, inflammation and disease
JID - 101635460
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Case-Control Studies
MH  - Cross-Sectional Studies
MH  - Humans
MH  - Immunoglobulin E
MH  - Nasal Mucosa
MH  - *Rhinitis, Allergic/genetics
MH  - *Transcriptome
PMC - PMC8669689
OTO - NOTNLM
OT  - epithelium
OT  - gene expression
OT  - mucosa
OT  - rhinitis
EDAT- 2021/10/13 06:00
MHDA- 2022/04/05 06:00
PMCR- 2021/10/12
CRDT- 2021/10/12 17:28
PHST- 2021/09/26 00:00 [revised]
PHST- 2021/07/14 00:00 [received]
PHST- 2021/09/29 00:00 [accepted]
PHST- 2021/10/13 06:00 [pubmed]
PHST- 2022/04/05 06:00 [medline]
PHST- 2021/10/12 17:28 [entrez]
PHST- 2021/10/12 00:00 [pmc-release]
AID - IID3545 [pii]
AID - 10.1002/iid3.545 [doi]
PST - ppublish
SO  - Immun Inflamm Dis. 2022 Jan;10(1):78-92. doi: 10.1002/iid3.545. Epub 2021 Oct 12.