PMID- 34640027 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20211016 IS - 1996-1944 (Print) IS - 1996-1944 (Electronic) IS - 1996-1944 (Linking) VI - 14 IP - 19 DP - 2021 Sep 28 TI - Mechanism of Sand Cementation with an Efficient Method of Microbial-Induced Calcite Precipitation. LID - 10.3390/ma14195631 [doi] LID - 5631 AB - This paper presents an efficient method of microbial-induced calcite precipitation (MICP) for cementation of sand particles. First, the influence of initial pH value of the culture medium on the growth of bacteria was discussed. Then, the compressive strength and calcium carbonate content of cemented sand columns with different sand particle sizes were measured to indicate the cementation effectiveness. The microstructure of cemented sand columns as well as the mineral composition and distribution of calcium carbonate were characterised by means of scanning electron microscopy-energy dispersive spectrometer (SEM-EDS) and X-ray diffraction (XRD). The results showed that the urease-producing bacteria S. pasteurii can be cultured at the initial pH values of 7-10, while a higher pH (e.g., 11) would hinder its growth and decrease its urease activity. The injection method of MICP with high standing time can cement sand columns effectively. Small average sand particle size of sand columns and high injection cycles can facilitate the gain of compressive strength, while calcium carbonate content of sand column higher than 8% can promote the increase of compressive strength. XRD results indicate that the fine grains generated on the surface of sand particles are calcite. The distribution of calcite on sand particles' surface is broad and uniform. First, calcite was precipitated on the surface of sand particles, and then a precipitation layer was formed, which would connect sand particles through its high enough thickness and contribute to the development of compressive strength of the whole sand column. FAU - Wang, Lu AU - Wang L AD - State Key Laboratory of Water Resources and Hydropower Engineering Science, Wuhan University, Wuhan 430072, China. FAU - Liu, Shuhua AU - Liu S AUID- ORCID: 0000-0002-8333-1045 AD - State Key Laboratory of Water Resources and Hydropower Engineering Science, Wuhan University, Wuhan 430072, China. LA - eng GR - 2020YFC1806401/National Key R&D Program of China/ GR - 2020010601012200/Application Foundation Frontier Project of Wuhan City/ GR - 2019SGG01/Visiting Researcher Fund Program of State Key Laboratory of Water Resources and Hydropower Engineering Science/ PT - Journal Article DEP - 20210928 PL - Switzerland TA - Materials (Basel) JT - Materials (Basel, Switzerland) JID - 101555929 PMC - PMC8510110 OTO - NOTNLM OT - calcium carbonate content OT - cemented sand OT - compressive strength OT - microbial-induced calcite precipitation OT - microstructure COIS- The authors declare no conflict of interest. EDAT- 2021/10/14 06:00 MHDA- 2021/10/14 06:01 PMCR- 2021/09/28 CRDT- 2021/10/13 01:09 PHST- 2021/08/21 00:00 [received] PHST- 2021/09/20 00:00 [revised] PHST- 2021/09/25 00:00 [accepted] PHST- 2021/10/13 01:09 [entrez] PHST- 2021/10/14 06:00 [pubmed] PHST- 2021/10/14 06:01 [medline] PHST- 2021/09/28 00:00 [pmc-release] AID - ma14195631 [pii] AID - materials-14-05631 [pii] AID - 10.3390/ma14195631 [doi] PST - epublish SO - Materials (Basel). 2021 Sep 28;14(19):5631. doi: 10.3390/ma14195631.