PMID- 34642260
OWN - NLM
STAT- MEDLINE
DCOM- 20220113
LR  - 20220531
IS  - 1899-1505 (Electronic)
IS  - 0867-5910 (Linking)
VI  - 72
IP  - 2
DP  - 2021 Apr
TI  - The factors involved in the induction of neutrophil gelatinase-associated 
      lipocalin overexpression in renal tubular epithelial cells under endoplasmic 
      reticulum stress.
LID - 10.26402/jpp.2021.2.14 [doi]
AB  - Our previous work found that neutrophil gelatinase-associated lipocalin (NGAL) 
      expression increases when endoplasmic reticulum stress (ERS) occurs in human 
      kidney-2 (HK-2) tubular epithelial cells. However, the reason for this is not yet 
      known. This study investigated the factors involved when inducing NGAL 
      overexpression in HK-2 cells during ERS. The cells were divided into six groups: 
      the control group (normal HK-2 cells), the ERS group (HK-2 cells cultured in 
      complete medium with thapsigargin (TG)), the transfection group (HK-2 cells 
      transfected with activating transcription factor 4 small interfering ribonucleic 
      acid (ATF4 siRNA), the ERS after transfection group (HK-2 cells transfected with 
      ATF4 siRNA, then cultured in complete medium with TG), the negative control group 
      (HK-2 cells transfected with siRNA-negative contrast), and the dimethyl sulfoxide 
      (DMSO) group (HK-2 cells cultured in complete medium with DMSO). Western blot and 
      a real-time polymerase chain reaction were used to measure the expression of 
      protein and messenger ribonucleic acid (mRNA). As a result NGAL, ATF4, C/EBP 
      homologous protein, glucose-regulated protein 78 kDa, ATF4 mRNA, and NGAL mRNA 
      were clearly overexpressed in the ERS group compared with the control group (p < 
      0.05). The expression of NGAL and ATF4 were similar in the control group, the 
      negative control group, and the DMSO group (p > 0.05). Meanwhile, ATF4, NGAL, 
      ATF4 mRNA, and NGAL mRNA in the ERS after transfection group were significantly 
      lower compared with the ERS group (p < 0.05), which showed that NGAL was affected 
      by ATF4. There was a close correlation between NGAL and ATF4; when the expression 
      of ATF4 was inhibited, NGAL was significantly lower. Therefore, ATF4 may be one 
      of the upstream regulators of NGAL.
FAU - Fu, T
AU  - Fu T
AD  - Department of Pediatrics, Shengjing Hospital of China Medical University, 
      Shenyang, China.
FAU - Hou, L
AU  - Hou L
AD  - Department of Pediatrics, Shengjing Hospital of China Medical University, 
      Shenyang, China.
FAU - Du, Y
AU  - Du Y
AD  - Department of Pediatrics, Shengjing Hospital of China Medical University, 
      Shenyang, China. drduyue24@163.com.
LA  - eng
PT  - Journal Article
DEP - 20210710
PL  - Poland
TA  - J Physiol Pharmacol
JT  - Journal of physiology and pharmacology : an official journal of the Polish 
      Physiological Society
JID - 9114501
RN  - 0 (Lipocalin-2)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - *Endoplasmic Reticulum Stress
MH  - *Epithelial Cells
MH  - Humans
MH  - Lipocalin-2/genetics
MH  - RNA, Messenger
MH  - Transfection
EDAT- 2021/10/14 06:00
MHDA- 2022/01/14 06:00
CRDT- 2021/10/13 06:21
PHST- 2021/03/14 00:00 [received]
PHST- 2021/04/30 00:00 [accepted]
PHST- 2021/10/13 06:21 [entrez]
PHST- 2021/10/14 06:00 [pubmed]
PHST- 2022/01/14 06:00 [medline]
AID - 10.26402/jpp.2021.2.14 [doi]
PST - ppublish
SO  - J Physiol Pharmacol. 2021 Apr;72(2). doi: 10.26402/jpp.2021.2.14. Epub 2021 Jul 
      10.