PMID- 34643087
OWN - NLM
STAT- MEDLINE
DCOM- 20211206
LR  - 20211214
IS  - 1522-1504 (Electronic)
IS  - 1040-0605 (Linking)
VI  - 321
IP  - 6
DP  - 2021 Dec 1
TI  - Optimization of long-term cold storage of rat precision-cut lung slices with a 
      tissue preservation solution.
PG  - L1023-L1035
LID - 10.1152/ajplung.00076.2021 [doi]
AB  - Precision-cut lung slices (PCLS) are used as ex vivo model of the lung to fill 
      the gap between in vitro and in vivo experiments. To allow optimal utilization of 
      PCLS, possibilities to prolong slice viability via cold storage using optimized 
      storage solutions were evaluated. Rat PCLS were cold stored in DMEM/F-12 or two 
      different preservation solutions for up to 28 days at 4 degrees C. After rewarming in 
      DMEM/F-12, metabolic activity, live/dead staining, and mitochondrial membrane 
      potential was assessed to analyze overall tissue viability. Single-cell 
      suspensions were prepared and proportions of CD45(+), EpCAM(+), CD31(+), and 
      CD90(+) cells were analyzed. As functional parameters, TNF-alpha expression was 
      analyzed to detect inflammatory activity and bronchoconstriction was evaluated 
      after acetylcholine stimulus. After 14 days of cold storage, viability and 
      mitochondrial membrane potential were significantly better preserved after 
      storage in solution 1 (potassium chloride rich) and solution 2 (potassium- and 
      lactobionate-rich analog) compared with DMEM/F-12. Analysis of cell populations 
      revealed efficient preservation of EpCAM(+), CD31(+), and CD90(+) cells. 
      Proportion of CD45(+) cells decreased during cold storage but was better 
      preserved by both modified solutions than by DMEM/F-12. PCLS stored in solution 1 
      responded substantially longer to inflammatory stimulation than those stored in 
      DMEM/F-12 or solution 2. Analysis of bronchoconstriction revealed total loss of 
      function after 14 days of storage in DMEM/F-12 but, in contrast, a good response 
      in PCLS stored in the optimized solutions. An improved base solution with a high 
      potassium chloride concentration optimizes cold storage of PCLS and allows 
      shipment between laboratories and stockpiling of tissue samples.
FAU - Tigges, Jonas
AU  - Tigges J
AD  - Bundeswehr Institute of Pharmacology and Toxicology, Munich, Germany.
FAU - Eggerbauer, Florian
AU  - Eggerbauer F
AD  - Walther Straub Institute of Pharmacology and Toxicology, Munich, Germany.
FAU - Worek, Franz
AU  - Worek F
AD  - Bundeswehr Institute of Pharmacology and Toxicology, Munich, Germany.
FAU - Thiermann, Horst
AU  - Thiermann H
AD  - Bundeswehr Institute of Pharmacology and Toxicology, Munich, Germany.
FAU - Rauen, Ursula
AU  - Rauen U
AD  - Institute of Physiological Chemistry, University Hospital, Essen, Germany.
FAU - Wille, Timo
AU  - Wille T
AUID- ORCID: 0000-0002-4359-5388
AD  - Bundeswehr Institute of Pharmacology and Toxicology, Munich, Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20211013
PL  - United States
TA  - Am J Physiol Lung Cell Mol Physiol
JT  - American journal of physiology. Lung cellular and molecular physiology
JID - 100901229
RN  - 0 (Organ Preservation Solutions)
SB  - IM
MH  - Animals
MH  - *Cold Temperature
MH  - Cryopreservation/*methods
MH  - Lung/*physiology
MH  - Male
MH  - *Membrane Potential, Mitochondrial
MH  - Organ Preservation Solutions/*chemistry
MH  - Rats
MH  - Rats, Wistar
MH  - Tissue Preservation/*methods
MH  - *Tissue Survival
OTO - NOTNLM
OT  - 3R
OT  - PCLS
OT  - cold storage
OT  - tissue preservation
EDAT- 2021/10/14 06:00
MHDA- 2021/12/15 06:00
CRDT- 2021/10/13 08:38
PHST- 2021/10/14 06:00 [pubmed]
PHST- 2021/12/15 06:00 [medline]
PHST- 2021/10/13 08:38 [entrez]
AID - 10.1152/ajplung.00076.2021 [doi]
PST - ppublish
SO  - Am J Physiol Lung Cell Mol Physiol. 2021 Dec 1;321(6):L1023-L1035. doi: 
      10.1152/ajplung.00076.2021. Epub 2021 Oct 13.