PMID- 34645309
OWN - NLM
STAT- MEDLINE
DCOM- 20220401
LR  - 20220401
IS  - 0191-2917 (Print)
IS  - 0191-2917 (Linking)
VI  - 106
IP  - 3
DP  - 2022 Mar
TI  - Identification and Evaluation of Wheat-Aegilops bicornis Lines with Resistance to 
      Powdery Mildew and Stripe Rust.
PG  - 864-871
LID - 10.1094/PDIS-05-21-0982-RE [doi]
AB  - Wheat pathogens, especially those causing powdery mildew and stripe rust, 
      seriously threaten yield worldwide. Utilizing newly identified disease resistance 
      genes from wheat relatives is an effective strategy to minimize disease damage. 
      In this study, chromosome-specific molecular markers for the 3S(b) and 7S(b) 
      chromosomes of Aegilops bicornis were developed using PCR-based landmark unique 
      gene primers for screening wheat-A. bicornis progenies. Fluorescence in situ 
      hybridization (FISH) was performed to further identify wheat-A. bicornis 
      progenies using oligonucleotides probes Oligo-pSc119.2-1, Oligo-pTa535-1, and 
      Oligo-(GAA)(8). After establishing A. bicornis 3S(b) and 7S(b) 
      chromosome-specific FISH markers, Holdfast (common wheat)-A. bicornis 3S(b) 
      addition, 7S(b) addition, 3S(b)(3A) substitution, 3S(b)(3B) substitution, 
      3S(b)(3D) substitution, 7S(b)(7A) substitution, and 7S(b)(7B) substitution lines 
      were identified by the molecular and cytological markers. Stripe rust and powdery 
      mildew resistance, along with agronomic traits, were investigated to evaluate the 
      breeding potential of these lines. Holdfast and Holdfast-A. bicornis progenies 
      were all highly resistant to stripe rust, indicating that the stripe rust 
      resistance might derive from Holdfast. However, Holdfast-A. bicornis 3S(b) 
      addition, 3S(b)(3A) substitution, 3S(b)(3B) substitution, and 3S(b)(3D) 
      substitution lines showed high resistance to powdery mildew while Holdfast was 
      highly susceptible, indicating that chromosome 3S(b) of A. bicornis carries 
      previously unknown powdery mildew resistance gene(s). Additionally, the transfer 
      of the 3S(b) chromosome from A. bicornis to wheat significantly increased tiller 
      number, but chromosome 7S(b) has a negative effect on agronomic traits. 
      Therefore, wheat germplasm containing A. bicornis chromosome 3S(b) has potential 
      to contribute to improving powdery mildew resistance and tiller number during 
      wheat breeding.
FAU - Wang, Xiaolu
AU  - Wang X
AUID- ORCID: 0000-0002-0271-2453
AD  - Key Laboratory of Wheat Biology and Genetic Improvement in the North Yellow and 
      Huai River Valley of Ministry of Agriculture, Crop Research Institute, Shandong 
      Academy of Agricultural Sciences, Jinan, Shandong 250100, China.
FAU - Han, Ran
AU  - Han R
AD  - Key Laboratory of Wheat Biology and Genetic Improvement in the North Yellow and 
      Huai River Valley of Ministry of Agriculture, Crop Research Institute, Shandong 
      Academy of Agricultural Sciences, Jinan, Shandong 250100, China.
FAU - Chen, Zhiwei
AU  - Chen Z
AD  - College of Agricultural, Shanxi Agricultural University, Taigu, Shanxi 030801, 
      China.
FAU - Li, Jianbo
AU  - Li J
AUID- ORCID: 0000-0002-1078-8319
AD  - Key Laboratory of Wheat Biology and Genetic Improvement in the North Yellow and 
      Huai River Valley of Ministry of Agriculture, Crop Research Institute, Shandong 
      Academy of Agricultural Sciences, Jinan, Shandong 250100, China.
FAU - Zhu, Tong
AU  - Zhu T
AD  - College of Life Science, Yantai University, Yantai, Shandong 264005, China.
FAU - Guo, Jun
AU  - Guo J
AD  - Key Laboratory of Wheat Biology and Genetic Improvement in the North Yellow and 
      Huai River Valley of Ministry of Agriculture, Crop Research Institute, Shandong 
      Academy of Agricultural Sciences, Jinan, Shandong 250100, China.
FAU - Xu, Wenjing
AU  - Xu W
AD  - Key Laboratory of Wheat Biology and Genetic Improvement in the North Yellow and 
      Huai River Valley of Ministry of Agriculture, Crop Research Institute, Shandong 
      Academy of Agricultural Sciences, Jinan, Shandong 250100, China.
FAU - Zi, Yan
AU  - Zi Y
AD  - Key Laboratory of Wheat Biology and Genetic Improvement in the North Yellow and 
      Huai River Valley of Ministry of Agriculture, Crop Research Institute, Shandong 
      Academy of Agricultural Sciences, Jinan, Shandong 250100, China.
FAU - Li, Faji
AU  - Li F
AD  - Key Laboratory of Wheat Biology and Genetic Improvement in the North Yellow and 
      Huai River Valley of Ministry of Agriculture, Crop Research Institute, Shandong 
      Academy of Agricultural Sciences, Jinan, Shandong 250100, China.
FAU - Zhai, Shengnan
AU  - Zhai S
AD  - Key Laboratory of Wheat Biology and Genetic Improvement in the North Yellow and 
      Huai River Valley of Ministry of Agriculture, Crop Research Institute, Shandong 
      Academy of Agricultural Sciences, Jinan, Shandong 250100, China.
FAU - Li, Haosheng
AU  - Li H
AD  - Key Laboratory of Wheat Biology and Genetic Improvement in the North Yellow and 
      Huai River Valley of Ministry of Agriculture, Crop Research Institute, Shandong 
      Academy of Agricultural Sciences, Jinan, Shandong 250100, China.
FAU - Liu, Jianjun
AU  - Liu J
AD  - Key Laboratory of Wheat Biology and Genetic Improvement in the North Yellow and 
      Huai River Valley of Ministry of Agriculture, Crop Research Institute, Shandong 
      Academy of Agricultural Sciences, Jinan, Shandong 250100, China.
FAU - Liu, Aifeng
AU  - Liu A
AD  - Key Laboratory of Wheat Biology and Genetic Improvement in the North Yellow and 
      Huai River Valley of Ministry of Agriculture, Crop Research Institute, Shandong 
      Academy of Agricultural Sciences, Jinan, Shandong 250100, China.
FAU - Cheng, Dungong
AU  - Cheng D
AD  - Key Laboratory of Wheat Biology and Genetic Improvement in the North Yellow and 
      Huai River Valley of Ministry of Agriculture, Crop Research Institute, Shandong 
      Academy of Agricultural Sciences, Jinan, Shandong 250100, China.
FAU - Song, Jianmin
AU  - Song J
AD  - Key Laboratory of Wheat Biology and Genetic Improvement in the North Yellow and 
      Huai River Valley of Ministry of Agriculture, Crop Research Institute, Shandong 
      Academy of Agricultural Sciences, Jinan, Shandong 250100, China.
FAU - Jia, Juqing
AU  - Jia J
AD  - College of Agricultural, Shanxi Agricultural University, Taigu, Shanxi 030801, 
      China.
FAU - Ma, Pengtao
AU  - Ma P
AUID- ORCID: 0000-0003-1826-0751
AD  - College of Life Science, Yantai University, Yantai, Shandong 264005, China.
FAU - Liu, Cheng
AU  - Liu C
AD  - Key Laboratory of Wheat Biology and Genetic Improvement in the North Yellow and 
      Huai River Valley of Ministry of Agriculture, Crop Research Institute, Shandong 
      Academy of Agricultural Sciences, Jinan, Shandong 250100, China.
LA  - eng
PT  - Journal Article
DEP - 20220310
PL  - United States
TA  - Plant Dis
JT  - Plant disease
JID - 9882809
SB  - IM
MH  - *Aegilops/genetics
MH  - Chromosomes, Plant/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Plant Breeding
MH  - Plant Diseases/genetics
MH  - Triticum/genetics
OTO - NOTNLM
OT  - Aegilops bicornis
OT  - FISH
OT  - agronomic traits
OT  - disease resistance
OT  - molecular marker
EDAT- 2021/10/15 06:00
MHDA- 2022/04/02 06:00
CRDT- 2021/10/14 05:24
PHST- 2021/10/15 06:00 [pubmed]
PHST- 2022/04/02 06:00 [medline]
PHST- 2021/10/14 05:24 [entrez]
AID - 10.1094/PDIS-05-21-0982-RE [doi]
PST - ppublish
SO  - Plant Dis. 2022 Mar;106(3):864-871. doi: 10.1094/PDIS-05-21-0982-RE. Epub 2022 
      Mar 10.