PMID- 34647460
OWN - NLM
STAT- MEDLINE
DCOM- 20211110
LR  - 20211110
IS  - 1530-6992 (Electronic)
IS  - 1530-6984 (Linking)
VI  - 21
IP  - 20
DP  - 2021 Oct 27
TI  - Selective Encapsulation of Therapeutic mRNA in Engineered Extracellular Vesicles 
      by DNA Aptamer.
PG  - 8563-8570
LID - 10.1021/acs.nanolett.1c01817 [doi]
AB  - Extracellular vesicles (EV)-based delivery of therapeutic mRNAs is challenged by 
      the low loading efficiency. In this study, we designed a DNA aptamer consisting 
      of two parts: the single strand part recognized the AUG region of target mRNA, 
      preventing mRNA from translation and ribosome assembly; and the double strand 
      part containing the elements recognized by the CD9-ZF (zinc finger) motifs, 
      sorting DNA aptamer-mRNA complex into CD9-ZF engineered EVs. In vitro and in vivo 
      studies revealed that the system could efficiently load functional mRNAs to the 
      EVs. Furthermore, adipose specific delivery of loaded Pgc1alpha mRNA via the strategy 
      could efficiently induce white adipocyte browning. Similarly, delivery of 
      interleukin-10 (Il-10) mRNA via the strategy had potent anti-inflammatory effect 
      in inflammatory bowel disease (IBD) mouse model. Together, our study has proposed 
      an efficient strategy to load therapeutic mRNAs of interest into EVs, which could 
      be used as a promising strategy for gene therapy.
FAU - Zhang, Siyan
AU  - Zhang S
AUID- ORCID: 0000-0002-9446-0053
AD  - Department of Ultrasound Diagnostics, Tangdu Hospital, Fourth Military Medical 
      University, 710038 Xi'an, People's Republic of China.
FAU - Dong, Yan
AU  - Dong Y
AD  - Department of Hematology, Tangdu Hospital, Fourth Military Medical University, 
      Xi'an 710038, People's Republic of China.
FAU - Wang, Yixiao
AU  - Wang Y
AD  - Department of Ultrasound Diagnostics, Tangdu Hospital, Fourth Military Medical 
      University, 710038 Xi'an, People's Republic of China.
FAU - Sun, Wenqi
AU  - Sun W
AD  - Department of Ultrasound Diagnostics, Tangdu Hospital, Fourth Military Medical 
      University, 710038 Xi'an, People's Republic of China.
FAU - Wei, Mengying
AU  - Wei M
AUID- ORCID: 0000-0002-0445-9586
AD  - The State Laboratory of Cancer Biology, Department of Biochemistry and Molecular 
      Biology, Fourth Military Medical University Xi'an, 710032, People's Republic of 
      China.
FAU - Yuan, Lijun
AU  - Yuan L
AUID- ORCID: 0000-0001-5651-2564
AD  - Department of Ultrasound Diagnostics, Tangdu Hospital, Fourth Military Medical 
      University, 710038 Xi'an, People's Republic of China.
FAU - Yang, Guodong
AU  - Yang G
AUID- ORCID: 0000-0002-6307-2963
AD  - The State Laboratory of Cancer Biology, Department of Biochemistry and Molecular 
      Biology, Fourth Military Medical University Xi'an, 710032, People's Republic of 
      China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20211014
PL  - United States
TA  - Nano Lett
JT  - Nano letters
JID - 101088070
RN  - 0 (Aptamers, Nucleotide)
RN  - 0 (RNA, Messenger)
SB  - IM
MH  - Animals
MH  - *Aptamers, Nucleotide
MH  - Cell Movement
MH  - Disease Models, Animal
MH  - *Extracellular Vesicles
MH  - Mice
MH  - RNA, Messenger/genetics
OTO - NOTNLM
OT  - DNA aptamer
OT  - Extracellular vesicles
OT  - browning of white adipocytes
OT  - encapsulation
OT  - engineering
OT  - inflammatory bowel disease
OT  - therapeutic mRNA
EDAT- 2021/10/15 06:00
MHDA- 2021/11/11 06:00
CRDT- 2021/10/14 08:36
PHST- 2021/10/15 06:00 [pubmed]
PHST- 2021/11/11 06:00 [medline]
PHST- 2021/10/14 08:36 [entrez]
AID - 10.1021/acs.nanolett.1c01817 [doi]
PST - ppublish
SO  - Nano Lett. 2021 Oct 27;21(20):8563-8570. doi: 10.1021/acs.nanolett.1c01817. Epub 
      2021 Oct 14.