PMID- 34648662
OWN - NLM
STAT- MEDLINE
DCOM- 20220504
LR  - 20220504
IS  - 1097-4652 (Electronic)
IS  - 0021-9541 (Linking)
VI  - 237
IP  - 1
DP  - 2022 Jan
TI  - Hyperthermia alters interleukin-6 production in response to lipopolysaccharide 
      via endoplasmic reticulum stress in bovine endometrial cells.
PG  - 1087-1099
LID - 10.1002/jcp.30604 [doi]
AB  - In the postpartum period, cows experience the uterine bacterial infection and 
      develop the endometritis. To eliminate bacteria and recover from endometritis, 
      endometrial epithelial and stromal cells secrete the cytokine and chemokine, such 
      as interleukin 6 (IL-6), IL-8, and monocyte chemotactic protein 1 (MCP1), to 
      recruit immune cells. Moreover, the symptom of endometritis is prolonged in 
      summer and we have recently indicated that hyperthermia suppresses and enhances 
      the IL-6 production in response to lipopolysaccharide (LPS) challenge in 
      endometrial epithelial and stromal cells, respectively. However, the mechanisms 
      for the opposite reaction of IL-6 secretion in response to LPS challenge in both 
      types of endometrial cells under hyperthermia conditions were still unclear. To 
      reveal these mechanisms, both types of endometrial cells were cultured with LPS 
      under the control (38.5 degrees C) or hyperthermia (40.5 degrees C) conditions and 
      comprehensively analyzed differential gene expressions of them by RNA-seq. In 
      addition, based on these results, we examined the effect of endoplasmic reticulum 
      (ER) stress on the IL-6 production in both types of endometrial cells cultured 
      with LPS under hyperthermia conditions. In comprehensive analysis, hyperthermia 
      induced the ER stress in the endometrial stromal cells but not in the endometrial 
      epithelial cells. Actually, we confirmed that hyperthermia increased the gene 
      expression of BiP, ATF4, and sXBP1 and protein expression of BiP and 
      phosphorylated inositol requiring 1, ER stress marker, in the endometrial stromal 
      cells but not in the endometrial epithelial cells. Moreover, in the endometrial 
      stromal cells exposed to LPS, activation and inhibition of ER stress enhanced the 
      IL-6 production under control conditions and suppressed it under hyperthermia 
      conditions, respectively. In this study, we could uncover the one of causes for 
      the disruption of IL-6 production in response to LPS challenge in the endometrial 
      cells under hyperthermia conditions. This finding might be a clue for the 
      improvement of the symptom of endometritis in cows during summer.
CI  - (c) 2021 Wiley Periodicals LLC.
FAU - Sakai, Shunsuke
AU  - Sakai S
AD  - Laboratory of Reproductive Physiology, Graduate School of Environmental and Life 
      Science, Okayama University, Okayama, Japan.
FAU - Inoue, Yuki
AU  - Inoue Y
AD  - Laboratory of Animal Reproduction, Department of Animal Science, Tokyo University 
      of Agriculture, Kanagawa, Japan.
FAU - Tanaka, Keisuke
AU  - Tanaka K
AD  - NODAI Genome Research Center, Tokyo University of Agriculture, Tokyo, Japan.
FAU - Yamamoto, Yuki
AU  - Yamamoto Y
AD  - Laboratory of Reproductive Physiology, Graduate School of Environmental and Life 
      Science, Okayama University, Okayama, Japan.
FAU - Iwata, Hisataka
AU  - Iwata H
AD  - Laboratory of Animal Reproduction, Department of Animal Science, Tokyo University 
      of Agriculture, Kanagawa, Japan.
FAU - Kimura, Koji
AU  - Kimura K
AUID- ORCID: 0000-0001-6153-6384
AD  - Laboratory of Reproductive Physiology, Graduate School of Environmental and Life 
      Science, Okayama University, Okayama, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20211014
PL  - United States
TA  - J Cell Physiol
JT  - Journal of cellular physiology
JID - 0050222
RN  - 0 (Interleukin-6)
RN  - 0 (Lipopolysaccharides)
SB  - IM
MH  - Animals
MH  - Cattle
MH  - *Endometritis/genetics
MH  - Endometrium/metabolism
MH  - Endoplasmic Reticulum Stress
MH  - Female
MH  - Gene Expression
MH  - Humans
MH  - *Hyperthermia, Induced
MH  - Interleukin-6/metabolism
MH  - Lipopolysaccharides/metabolism/pharmacology
OTO - NOTNLM
OT  - IL-6
OT  - cattle
OT  - endometrial cells
OT  - endoplasmic reticulum stress
OT  - heat stress
OT  - hyperthermia
EDAT- 2021/10/15 06:00
MHDA- 2022/05/06 06:00
CRDT- 2021/10/14 17:26
PHST- 2021/09/12 00:00 [revised]
PHST- 2021/04/26 00:00 [received]
PHST- 2021/09/15 00:00 [accepted]
PHST- 2021/10/15 06:00 [pubmed]
PHST- 2022/05/06 06:00 [medline]
PHST- 2021/10/14 17:26 [entrez]
AID - 10.1002/jcp.30604 [doi]
PST - ppublish
SO  - J Cell Physiol. 2022 Jan;237(1):1087-1099. doi: 10.1002/jcp.30604. Epub 2021 Oct 
      14.