PMID- 34648856
OWN - NLM
STAT- MEDLINE
DCOM- 20220221
LR  - 20220221
IS  - 1615-5947 (Electronic)
IS  - 0890-5096 (Linking)
VI  - 79
DP  - 2022 Feb
TI  - Phenotypic and Functional Transformation in Smooth Muscle Cells Derived from a 
      Superficial Thrombophlebitis-affected Vein Wall.
PG  - 335-347
LID - S0890-5096(21)00688-9 [pii]
LID - 10.1016/j.avsg.2021.09.016 [doi]
AB  - BACKGROUND: Superficial thrombophlebitis (ST) is a frequent pathology, but its 
      exact incidence remains to be determined. This study tested the hypothesis 
      whether relationships exist among smooth muscle cells (SMCs) derived from ST, 
      varicose great saphenous veins (VGSVs), and normal great saphenous veins (GSVs). 
      METHODS: Forty-one samples of ST, VGSVs, and GSVs were collected. SMCs were 
      isolated and cultured. Proliferation, migration, adhesion, and senescence in SMCs 
      from the three vein walls were compared by various methods. Bax, Bcl-2, 
      caspase-3, matrix metalloproteinase-2 (MMP-2), MMP-9, tissue inhibitor of 
      metalloproteinase-1 (TIMP-1), and TIMP-2 messenger RNA (mRNA) and protein 
      expressions were detected by fluorescence quantitative PCR and Western blot. 
      RESULTS: An obvious decrease in cytoskeletal filaments was observed in 
      thrombophlebitic vascular smooth muscle cells (TVSMCs). The quantity of 
      proliferation, migration, adhesion, and senescence in TVSMCs was significantly 
      higher than in varicose vascular smooth muscle cells and normal vascular smooth 
      muscle cells (NVSMCs) (all P < 0.05). Bax and caspase-3 mRNA and protein 
      expression were decreased, while Bcl-2 mRNA and protein expression were increased 
      in the TVSMCs compared with the varicose vascular smooth muscle cells and the 
      NVSMCs (all P < 0.05). MMP-2, MMP-9, TIMP-1, and TIMP-2 mRNA and protein 
      expression were significantly increased in the TVSMCs compared with the VVGSVs 
      and the NVSMCs (all P < 0.05). CONCLUSION: SMCs derived from ST are more 
      dedifferentiated and demonstrate increased cell proliferation, migration, 
      adhesion, and senescence, as well as obviously decreased cytoskeletal filaments. 
      These results suggest that the phenotypic and functional differences could be 
      related to the presence of atrophic and hypertrophic vein segments during the 
      disease course among SMCs derived from ST, VGSVs, and GSVs.
CI  - Copyright (c) 2021. Published by Elsevier Inc.
FAU - Li, Kun
AU  - Li K
AD  - Center of General Surgery, The 80th Group Army Hospital of People's Liberation 
      Army, Weifang, China.; State Key Laboratory of Toxicology and Medical 
      Countermeasures, Beijing Institute of Pharmacology and Toxicology, Beijing, 
      China.
FAU - Yu, Guoting
AU  - Yu G
AD  - Center of General Surgery, The 80th Group Army Hospital of People's Liberation 
      Army, Weifang, China.; State Key Laboratory of Toxicology and Medical 
      Countermeasures, Beijing Institute of Pharmacology and Toxicology, Beijing, 
      China.
FAU - Xu, Yongbo
AU  - Xu Y
AD  - Center of General Surgery, The 80th Group Army Hospital of People's Liberation 
      Army, Weifang, China.
FAU - Chu, Haibo
AU  - Chu H
AD  - Center of General Surgery, The 80th Group Army Hospital of People's Liberation 
      Army, Weifang, China.
FAU - Zhong, Yuxu
AU  - Zhong Y
AD  - State Key Laboratory of Toxicology and Medical Countermeasures, Beijing Institute 
      of Pharmacology and Toxicology, Beijing, China.. Electronic address: 
      yuxuzhong2008@aliyun.com.
FAU - Zhan, Hanxiang
AU  - Zhan H
AD  - Department of General Surgery, Qilu Hospital, Shandong University, Jinan, China.. 
      Electronic address: zhanhanxiang@126.com.
CN  - Weifang and Jinan, Shandong; Beijing, China
LA  - eng
PT  - Journal Article
DEP - 20211011
PL  - Netherlands
TA  - Ann Vasc Surg
JT  - Annals of vascular surgery
JID - 8703941
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - 0 (TIMP1 protein, human)
RN  - 0 (TIMP2 protein, human)
RN  - 0 (Tissue Inhibitor of Metalloproteinase-1)
RN  - 127497-59-0 (Tissue Inhibitor of Metalloproteinase-2)
RN  - EC 3.4.24.24 (MMP2 protein, human)
RN  - EC 3.4.24.24 (Matrix Metalloproteinase 2)
RN  - EC 3.4.24.35 (MMP9 protein, human)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
SB  - IM
MH  - Apoptosis Regulatory Proteins/genetics/metabolism
MH  - Case-Control Studies
MH  - Cell Adhesion
MH  - *Cell Dedifferentiation
MH  - Cell Movement
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Cellular Senescence
MH  - Cytoskeleton/metabolism/*pathology
MH  - Female
MH  - Humans
MH  - Male
MH  - Matrix Metalloproteinase 2/genetics/metabolism
MH  - Matrix Metalloproteinase 9/genetics/metabolism
MH  - Middle Aged
MH  - Muscle, Smooth, Vascular/metabolism/*pathology
MH  - Myocytes, Smooth Muscle/metabolism/*pathology
MH  - Phenotype
MH  - Saphenous Vein/metabolism/pathology
MH  - Thrombophlebitis/genetics/metabolism/*pathology
MH  - Tissue Inhibitor of Metalloproteinase-1/genetics/metabolism
MH  - Tissue Inhibitor of Metalloproteinase-2/genetics/metabolism
MH  - Varicose Veins/genetics/metabolism/*pathology
EDAT- 2021/10/15 06:00
MHDA- 2022/02/22 06:00
CRDT- 2021/10/14 20:12
PHST- 2021/07/22 00:00 [received]
PHST- 2021/08/30 00:00 [revised]
PHST- 2021/09/05 00:00 [accepted]
PHST- 2021/10/15 06:00 [pubmed]
PHST- 2022/02/22 06:00 [medline]
PHST- 2021/10/14 20:12 [entrez]
AID - S0890-5096(21)00688-9 [pii]
AID - 10.1016/j.avsg.2021.09.016 [doi]
PST - ppublish
SO  - Ann Vasc Surg. 2022 Feb;79:335-347. doi: 10.1016/j.avsg.2021.09.016. Epub 2021 
      Oct 11.