PMID- 34655587
OWN - NLM
STAT- MEDLINE
DCOM- 20211217
LR  - 20211217
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
VI  - 192
DP  - 2021 Dec 1
TI  - Fabrication of stable electrospun blended chitosan-poly(vinyl alcohol) nanofibers 
      for designing naked-eye colorimetric glucose biosensor based on GOx/HRP.
PG  - 999-1012
LID - S0141-8130(21)02200-5 [pii]
LID - 10.1016/j.ijbiomac.2021.10.048 [doi]
AB  - In this study, designing of a stable electrospun blended chitosan (CS)-poly(vinyl 
      alcohol) (PVA) nanofibers for colorimetric glucose biosensing in an aqueous 
      medium was investigated. CS and PVA solutions were blended to acquire an optimum 
      content (CS/PVA:1/4) and electrospunned to obtain uniform and bead-free CS/PVA 
      nanofiber structures following the optimization of the electrospinning parameters 
      (33 kV, 20 cm, and 1.2 ml.h(-1)). Crosslinking process applied subsequently 
      provided mechanically and chemically stable nanofibers with an average diameter 
      of 378 nm. The morphological homogeneity, high fluid absorption ability (>%50), 
      thermal (<230  degrees C) and morphological stability, surface hydrophilicity and 
      degrability properties of cross-linked CS/PVA nanofiber demonstrated their great 
      potential to be developed as an eye-readable strip for biosensing applications. 
      The glucose oxidase (GOx) and horseradish peroxidase (HRP) was immobilized by 
      physical adsorption on the cross-linked CS/PVA nanofiber. The glucose assay 
      analysis by ultraviolet-visible (UV-Vis) spectrophotometry using the same 
      enzymatic system of the proposed glucose strips in form of absorbance versus 
      concentration plot was found to be linear over a glucose concentration range of 
      2.7 to 13.8 mM. The prepared naked eye colorimetric glucose detection strips, 
      with lower detection limit of 2.7 mM, demonstrated dramatic color change from 
      white (0 mM) to brownish-orange (13.8 mM). The developed cross-linked CS/PVA 
      nanofiber strips, prepared by electrospinnig procedure, could be easily adapted 
      to a color map, as an alternative material for glucose sensing. Design of a 
      practical, low-cost, and environmental-friendly bio-based CS/PVA testing strips 
      for eye readable detection were presented and suggested as an applicable medium 
      for a wide range of glucose concentrations.
CI  - Copyright (c) 2021 Elsevier B.V. All rights reserved.
FAU - Coskuner Filiz, Bilge
AU  - Coskuner Filiz B
AD  - Yildiz Technical University, Metallurgy and Materials Engineering Department, 
      Istanbul 34210, Turkey. Electronic address: coskuner@yildiz.edu.tr.
FAU - Basaran Elalmis, Yeliz
AU  - Basaran Elalmis Y
AD  - Yildiz Technical University, Bioengineering Department, Istanbul 34210, Turkey.
FAU - Bektas, Irem Serra
AU  - Bektas IS
AD  - Yildiz Technical University, Chemical Engineering Department, Istanbul 34210, 
      Turkey.
FAU - Kanturk Figen, Aysel
AU  - Kanturk Figen A
AD  - Yildiz Technical University, Chemical Engineering Department, Istanbul 34210, 
      Turkey.
LA  - eng
PT  - Journal Article
DEP - 20211013
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - 0 (Enzymes, Immobilized)
RN  - 9002-89-5 (Polyvinyl Alcohol)
RN  - 9012-76-4 (Chitosan)
RN  - EC 1.1.3.4 (Glucose Oxidase)
RN  - EC 1.11.1.- (Horseradish Peroxidase)
RN  - IY9XDZ35W2 (Glucose)
SB  - IM
MH  - *Biosensing Techniques
MH  - Chemical Phenomena
MH  - Chitosan/*chemistry
MH  - Colorimetry/*methods
MH  - Enzymes, Immobilized
MH  - Glucose/*analysis
MH  - Glucose Oxidase/chemistry
MH  - Horseradish Peroxidase
MH  - Hydrogen-Ion Concentration
MH  - Nanofibers/*chemistry/ultrastructure
MH  - Nanotechnology
MH  - Polyvinyl Alcohol/*chemistry
MH  - Spectrum Analysis
OTO - NOTNLM
OT  - Biosensing
OT  - Chitosan/polyvinyl alcohol
OT  - Colorimetric
OT  - Electrospinning
OT  - Glucose
OT  - Nanofiber
EDAT- 2021/10/17 06:00
MHDA- 2021/12/18 06:00
CRDT- 2021/10/16 20:10
PHST- 2021/07/14 00:00 [received]
PHST- 2021/09/14 00:00 [revised]
PHST- 2021/10/07 00:00 [accepted]
PHST- 2021/10/17 06:00 [pubmed]
PHST- 2021/12/18 06:00 [medline]
PHST- 2021/10/16 20:10 [entrez]
AID - S0141-8130(21)02200-5 [pii]
AID - 10.1016/j.ijbiomac.2021.10.048 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2021 Dec 1;192:999-1012. doi: 
      10.1016/j.ijbiomac.2021.10.048. Epub 2021 Oct 13.