PMID- 34655847
OWN - NLM
STAT- MEDLINE
DCOM- 20220321
LR  - 20220321
IS  - 1878-1705 (Electronic)
IS  - 1567-5769 (Linking)
VI  - 101
IP  - Pt B
DP  - 2021 Dec
TI  - Extracellular vesicles secreted from mesenchymal stem cells exert anti-apoptotic 
      and anti-inflammatory effects via transmitting microRNA-18b in rats with diabetic 
      retinopathy.
PG  - 108234
LID - S1567-5769(21)00870-5 [pii]
LID - 10.1016/j.intimp.2021.108234 [doi]
AB  - Diabetic retinopathy (DR) is a major cause of visual deficits and blindness in 
      the working-age population and inflammatory response is a key event during DR. In 
      this study, we investigated the anti-inflammatory properties of small 
      extracellular vesicles (sEVs) derived from human umbilical cord mesenchymal stem 
      cells (hUCMSCs) in a diabetic rat model and human retinal microvascular 
      endothelial cells. After development of DR in rats subjected to diabetes 
      induction with streptozotocin (STZ), the DR rats were treated with different 
      concentrations of hUCMSC-sEVs. Our results showed that the treatment of the 
      retinas of DR rats with hUCMSC-sEVs not only reduced the level of vascular 
      leakage in the retinas of rats but also decreased the retinal thickness as well 
      as the associated inflammation. Further, our in vitro evidences suggest that 
      hUCMSC-sEVs repress high glucose (HG)-induced cell inflammation and apoptosis. 
      Subsequently, we analyzed the differentially expressed microRNAs (miRNAs) in the 
      hUCMSC-sEVs by microarray and performed in silico studies to predict the target 
      mRNA of miR-18b. Our findings also revealed that the expression of miR-18b was 
      significantly elevated in the retina of diabetic rats after sEV treatment. In 
      addition, miR-18b was found to target mitogen-activated protein kinase kinase 
      kinase 1 (MAP3K1), thereby inhibiting NF-kappaB p65 phosphorylation to alleviate DR. 
      Overall, this study highlights the potential of hUCMSCs-sEVs as biomaterials for 
      anti-inflammatory and anti-apoptotic effects in DR by transferring miR-18b.
CI  - Copyright (c) 2021 Elsevier B.V. All rights reserved.
FAU - Xu, Zepeng
AU  - Xu Z
AD  - Department of Ophthalmology, Jiangmen Wuyi Hospital of TCM, Jiangmen 529000, 
      Guangdong, China; The First Clinical Medical College of Guangzhou University of 
      Chinese Medicine, Guangzhou 510504, Guangdong, China.
FAU - Tian, Ni
AU  - Tian N
AD  - Department of Ophthalmology, The First Affiliated Hospital of Guangzhou 
      University of Chinese Medicine, Guangzhou 510504, Guangdong, China. Electronic 
      address: tianni5191@126.com.
FAU - Li, Songtao
AU  - Li S
AD  - The First Clinical Medical College of Guangzhou University of Chinese Medicine, 
      Guangzhou 510504, Guangdong, China.
FAU - Li, Kunmeng
AU  - Li K
AD  - The First Clinical Medical College of Guangzhou University of Chinese Medicine, 
      Guangzhou 510504, Guangdong, China.
FAU - Guo, Haike
AU  - Guo H
AD  - Department of Ophthalmology, Shanghai Heping Eye Hospital, Shanghai 200437, 
      China.
FAU - Zhang, Hongyang
AU  - Zhang H
AD  - Department of Ophthalmology, Guangdong Provincial People's Hospital, Guangzhou 
      510120, Guangdong, China.
FAU - Jin, Haiying
AU  - Jin H
AD  - Department of Ophthalmology, Shanghai No. 10 People's Hospital, Shanghai 200040, 
      China.
FAU - An, Meixia
AU  - An M
AD  - Department of Ophthalmology, The Third Affiliated Hospital of Southern Medical 
      University, Guangzhou 510630, Guangdong, China.
FAU - Yu, Xiaoyi
AU  - Yu X
AD  - Department of Ophthalmology, The First Affiliated Hospital of Guangzhou 
      University of Chinese Medicine, Guangzhou 510504, Guangdong, China.
LA  - eng
PT  - Journal Article
DEP - 20211013
PL  - Netherlands
TA  - Int Immunopharmacol
JT  - International immunopharmacology
JID - 100965259
RN  - 0 (Blood Glucose)
RN  - 0 (MIRN18 microRNA, rat)
RN  - 0 (MicroRNAs)
SB  - IM
MH  - Animals
MH  - *Apoptosis
MH  - Aqueous Humor
MH  - Blood Glucose
MH  - Diabetes Mellitus, Experimental/complications
MH  - Diabetic Retinopathy/*therapy
MH  - *Extracellular Vesicles
MH  - Inflammation/*therapy
MH  - Male
MH  - Mesenchymal Stem Cells/*physiology
MH  - MicroRNAs/genetics/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Retina/pathology
OTO - NOTNLM
OT  - Diabetic retinopathy
OT  - Human umbilical cord mesenchymal stem cells
OT  - MAP3K1
OT  - NF-kappaB p65
OT  - Small extracellular vesicles
OT  - microRNA-18b
EDAT- 2021/10/17 06:00
MHDA- 2022/03/22 06:00
CRDT- 2021/10/16 20:15
PHST- 2021/06/15 00:00 [received]
PHST- 2021/09/14 00:00 [revised]
PHST- 2021/10/04 00:00 [accepted]
PHST- 2021/10/17 06:00 [pubmed]
PHST- 2022/03/22 06:00 [medline]
PHST- 2021/10/16 20:15 [entrez]
AID - S1567-5769(21)00870-5 [pii]
AID - 10.1016/j.intimp.2021.108234 [doi]
PST - ppublish
SO  - Int Immunopharmacol. 2021 Dec;101(Pt B):108234. doi: 
      10.1016/j.intimp.2021.108234. Epub 2021 Oct 13.