PMID- 34663065
OWN - NLM
STAT- MEDLINE
DCOM- 20220209
LR  - 20220408
IS  - 1944-8252 (Electronic)
IS  - 1944-8244 (Print)
IS  - 1944-8244 (Linking)
VI  - 13
IP  - 43
DP  - 2021 Nov 3
TI  - Controlled Self-Assembly of DNA-Mimicking Nanotubes to Form a Layer-by-Layer 
      Scaffold for Homeostatic Tissue Constructs.
PG  - 51321-51332
LID - 10.1021/acsami.1c13345 [doi]
AB  - Various biomaterial scaffolds have been developed for improving stem cell 
      anchorage and function in tissue constructs for in vitro and in vivo uses. Growth 
      factors are typically applied to scaffolds to mediate cell differentiation. 
      Conventionally, growth factors are not strictly localized in the scaffolds; thus, 
      they may leak into the surrounding environment, causing undesired side effects on 
      tissues or cells. Hence, there is a need for improved tissue construct strategies 
      based on highly localized drug delivery and a homeostatic microenvironment. This 
      study developed an injectable nanomatrix (NM) scaffold with a layer-by-layer 
      structure inside each nanosized fiber of the scaffold based on controlled 
      self-assembly at the molecular level. The NM was hierarchically assembled from 
      Janus base nanotubes (JBNTs), matrilin-3, and transforming growth factor beta-1 
      (TGF-beta1) via bioaffinity. JBNTs, which form the NM backbone, are novel 
      DNA-inspired nanomaterials that mimic the natural helical nanostructures of 
      collagens. The chondrogenic factor, TGF-beta1, was enveloped in the inner layer 
      inside the NM fibers to prevent its release. Matrilin-3 was incorporated into the 
      outer layer to create a cartilage-mimicking microenvironment and to maintain 
      tissue homeostasis. Interestingly, human mesenchymal stem cells (hMSCs) had a 
      strong preference to anchor along the NM fibers and formed a localized 
      homeostatic microenvironment. Therefore, this NM has successfully generated 
      highly organized structures via molecular self-assembly and achieved localized 
      drug delivery and stem cell anchorage for homeostatic tissue constructs.
FAU - Zhou, Libo
AU  - Zhou L
AD  - Department of Biomedical Engineering, University of Connecticut, Storrs, 
      Connecticut 06269, United States.
FAU - Zhang, Wuxia
AU  - Zhang W
AD  - Department of Biomedical Engineering, University of Connecticut, Storrs, 
      Connecticut 06269, United States.
FAU - Lee, Jinhyung
AU  - Lee J
AD  - Department of Biomedical Engineering, University of Connecticut, Storrs, 
      Connecticut 06269, United States.
FAU - Kuhn, Liisa
AU  - Kuhn L
AUID- ORCID: 0000-0003-1447-0719
AD  - Department of Biomedical Engineering, University of Connecticut, Storrs, 
      Connecticut 06269, United States.
FAU - Chen, Yupeng
AU  - Chen Y
AUID- ORCID: 0000-0001-6940-6277
AD  - Department of Biomedical Engineering, University of Connecticut, Storrs, 
      Connecticut 06269, United States.
LA  - eng
GR  - R01 AR072027/AR/NIAMS NIH HHS/United States
GR  - R03 AR069383/AR/NIAMS NIH HHS/United States
PT  - Journal Article
DEP - 20211019
PL  - United States
TA  - ACS Appl Mater Interfaces
JT  - ACS applied materials & interfaces
JID - 101504991
RN  - 0 (Biocompatible Materials)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Biocompatible Materials/*chemistry
MH  - DNA/*chemistry
MH  - Drug Delivery Systems
MH  - Homeostasis
MH  - Humans
MH  - Mesenchymal Stem Cells/cytology
MH  - Models, Molecular
MH  - Nanotubes/*chemistry
MH  - Particle Size
MH  - Surface Properties
MH  - *Tissue Engineering
MH  - Tissue Scaffolds/*chemistry
PMC - PMC8982526
MID - NIHMS1787758
OTO - NOTNLM
OT  - DNA nanotechnology
OT  - Janus base nanomatrix
OT  - layer-by-layer
OT  - self-assembly
OT  - tissue engineering
COIS- The authors declare the following competing financial interest(s): Dr. Yupeng 
      Chen is a co-founder of NanoDe Therapeutics, Inc.
EDAT- 2021/10/20 06:00
MHDA- 2022/02/10 06:00
PMCR- 2022/04/05
CRDT- 2021/10/19 05:27
PHST- 2021/10/20 06:00 [pubmed]
PHST- 2022/02/10 06:00 [medline]
PHST- 2021/10/19 05:27 [entrez]
PHST- 2022/04/05 00:00 [pmc-release]
AID - 10.1021/acsami.1c13345 [doi]
PST - ppublish
SO  - ACS Appl Mater Interfaces. 2021 Nov 3;13(43):51321-51332. doi: 
      10.1021/acsami.1c13345. Epub 2021 Oct 19.