PMID- 34663465 OWN - NLM STAT- MEDLINE DCOM- 20211108 LR - 20211108 IS - 1297-9716 (Electronic) IS - 0928-4249 (Print) IS - 0928-4249 (Linking) VI - 52 IP - 1 DP - 2021 Oct 18 TI - Expression of cytokines in dairy cattle mammary gland parenchyma during chronic staphylococcal infection. PG - 132 LID - 10.1186/s13567-021-01003-y [doi] LID - 132 AB - The study aim was to determine the expression of genes potentially related to chronic mastitis at the mRNA and protein levels, viz. chemokine C-C motif receptor 1 (CCR1), C-C motif chemokine ligand 2 (CCL2), C-C motif chemokine ligand 5 (CXCL5), tumor necrosis factor alpha (TNFalpha), interleukin 1beta (IL-1beta), interleukin 6 (IL-6), interleukin 8 (IL-8), interleukin 18 (IL-18), in bovine mammary gland parenchyma. The study examines the differences in expression of selected genes between cows with chronic mastitis caused by coagulase-positive (CoPS) or coagulase-negative staphylococci (CoNS) and those with healthy udders (H). Samples were collected from the udder quarters from 40 Polish Holstein-Friesian cows; 54 of these samples were chosen for analysis based on microbiological analysis of milk taken two days before slaughter. They were categorized into three groups: CoPS (N = 27), CoNS (N = 14) and H (N = 13). The RNA expression was analyzed by RT-qPCR and protein concentration by ELISA. No differences in the mRNA levels of seven genes (TNFalpha, IL-18, CCR1, IL-1beta, CCL2, IL-8, IL-6) and four proteins (TNFalpha, IL-18, CCR1, IL-1beta) were identified between the CoPS and H groups. Higher transcript levels of CXCL5 (p