PMID- 34664576 OWN - NLM STAT- MEDLINE DCOM- 20220131 LR - 20220131 IS - 2042-650X (Electronic) IS - 2042-6496 (Linking) VI - 12 IP - 20 DP - 2021 Oct 19 TI - Ultrasound-assisted enzymatic extraction of Corni Fructus alpha-glucosidase inhibitors improves insulin resistance in HepG2 cells. PG - 9808-9819 LID - 10.1039/d1fo01002f [doi] AB - Corni Fructus (CF) is a traditional medicine and beneficial food with multifaceted protective effects against diabetes and its complications. Since alpha-glucosidase inhibitors (GIs) are promising first-choice oral antihyperglycemic drugs for diabetes, we examined whether GIs from CF (GICF) are useful for diabetes treatment. Therefore, GICF was extracted by ultrasound-assisted enzymatic extraction (UAEE) that is optimized by a three-level, four-factor Box-Behnken design and determined by ultra-performance liquid chromatography. Compared to 36.31 mg g(-1) without enzyme treatment, the GICF yield increased to 70.44 mg g(-1)via UAEE under optimum conditions (0.5% compound enzyme extracted in 23 min at 46 degrees C and pH 4.8). The activity (91.99%) of GICF was as predicted (93.28%). When GICF was used in an insulin-resistant HepG2 cell model, it significantly ameliorated the glucose metabolism in a dose-dependent manner. Our findings indicate that UAEE may be an innovative method for functional food extraction and a potential strategy for high-quality food ingredient (such as GI) production with high efficiency and productivity. FAU - Han, Binkai AU - Han B AD - National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, The Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, The Ministry of Education, College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi 710119, The People's Republic of China. xuexch@snnu.edu.cn. FAU - Sun, Haoqiang AU - Sun H AD - National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, The Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, The Ministry of Education, College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi 710119, The People's Republic of China. xuexch@snnu.edu.cn. FAU - Yang, Xiaolin AU - Yang X AD - National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, The Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, The Ministry of Education, College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi 710119, The People's Republic of China. xuexch@snnu.edu.cn. FAU - Wang, Ting AU - Wang T AD - National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, The Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, The Ministry of Education, College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi 710119, The People's Republic of China. xuexch@snnu.edu.cn. FAU - He, Changfen AU - He C AD - National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, The Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, The Ministry of Education, College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi 710119, The People's Republic of China. xuexch@snnu.edu.cn. FAU - Zhao, Ke AU - Zhao K AD - National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, The Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, The Ministry of Education, College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi 710119, The People's Republic of China. xuexch@snnu.edu.cn. FAU - An, Shujing AU - An S AD - National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, The Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, The Ministry of Education, College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi 710119, The People's Republic of China. xuexch@snnu.edu.cn. FAU - Guo, Chen AU - Guo C AUID- ORCID: 0000-0001-6099-9889 AD - National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, The Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, The Ministry of Education, College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi 710119, The People's Republic of China. xuexch@snnu.edu.cn. FAU - Niu, Dou AU - Niu D AD - National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, The Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, The Ministry of Education, College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi 710119, The People's Republic of China. xuexch@snnu.edu.cn. FAU - Xue, Xiaochang AU - Xue X AD - National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, The Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, The Ministry of Education, College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi 710119, The People's Republic of China. xuexch@snnu.edu.cn. FAU - Kang, Jiefang AU - Kang J AUID- ORCID: 0000-0003-2809-3934 AD - National Engineering Laboratory for Resource Development of Endangered Crude Drugs in Northwest China, The Key Laboratory of Medicinal Resources and Natural Pharmaceutical Chemistry, The Ministry of Education, College of Life Sciences, Shaanxi Normal University, Xi'an, Shaanxi 710119, The People's Republic of China. xuexch@snnu.edu.cn. LA - eng PT - Journal Article DEP - 20211019 PL - England TA - Food Funct JT - Food & function JID - 101549033 RN - 0 (Glycoside Hydrolase Inhibitors) RN - 0 (Hypoglycemic Agents) RN - EC 3.2.1.- (Glycoside Hydrolases) RN - EC 3.2.1.- (hemicellulase) RN - EC 3.2.1.15 (Polygalacturonase) RN - EC 3.2.1.4 (Cellulase) SB - IM MH - Cellulase/isolation & purification MH - Chromatography, Liquid/methods MH - Cornus/*chemistry MH - Diabetes Mellitus/*drug therapy/metabolism MH - Glycoside Hydrolase Inhibitors/*isolation & purification/*pharmacology MH - Glycoside Hydrolases/isolation & purification MH - Hep G2 Cells MH - Humans MH - Hypoglycemic Agents/pharmacology MH - *Insulin Resistance MH - Polygalacturonase/isolation & purification MH - Ultrasonography/methods EDAT- 2021/10/20 06:00 MHDA- 2022/02/01 06:00 CRDT- 2021/10/19 08:45 PHST- 2021/10/19 08:45 [entrez] PHST- 2021/10/20 06:00 [pubmed] PHST- 2022/02/01 06:00 [medline] AID - 10.1039/d1fo01002f [doi] PST - epublish SO - Food Funct. 2021 Oct 19;12(20):9808-9819. doi: 10.1039/d1fo01002f.