PMID- 34667726
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211022
IS  - 2222-3959 (Print)
IS  - 2227-4898 (Electronic)
IS  - 2222-3959 (Linking)
VI  - 14
IP  - 10
DP  - 2021
TI  - Protective effect of LIF-huMSCs on the retina of diabetic model rats.
PG  - 1508-1517
LID - 10.18240/ijo.2021.10.06 [doi]
AB  - AIM: To investigate the protective effect of human umbilical cord mesenchymal 
      stem cells (hUCMSCs) modified by the LIF gene on the retinal function of diabetic 
      model rats and preliminarily explore the possible mechanism. METHODS: A stably 
      transfected cell line of hUCMSCs overexpressing leukemia inhibitory factor (LIF) 
      was constructed. Overexpression was verified by fluorescent quantitative 
      polymerase chain reaction (qPCR). Forty-eight adult Sprague-Dawley rats were 
      randomly divided into a normal control group (group A), streptozotocin-induced 
      diabetic control group (group B), diabetic rats at 3mo injected with empty 
      vector-transfected hUCMSCs (group C) or injected with LIF-hUCMSCs (group D). Four 
      weeks after the intravitreal injection, analyses in all groups included retinal 
      function using flash electroretinogram (F-ERG), retinal blood vessel examination 
      of retinal flat mounts perfused with fluorescein isothiocyanate-dextran 
      (FITC-dextran), and retinal structure examination of sections using hematoxylin 
      and eosin staining. Expression levels of adiponectin (APN), high-sensitivity 
      C-reactive protein (hs-CRP), and neurotrophin-4 (NT-4) in each group was detected 
      using immunohistochemistry, PCR, Western blotting, and ELISA, respectively. 
      RESULTS: A stable transgenic cell line of LIF-hUCMSCs was constructed. F-ERG and 
      FITC-dextran examinations revealed no abnormalities of retinal structure and 
      function in group A, severe damage of the retinal blood vessels and function in 
      group B, and improved retinal structure and function in group C and especially 
      group D. qPCR, ELISA, and Western blot analyses revealed progressively higher APN 
      and NT-4 expression levels in groups B, C, and D than in group A. hs-CRP 
      expression was significantly higher in group B than in groups A, C, and D, and 
      was significantly higher in group C than in group D (P<0.05). CONCLUSION: 
      LIF-hUCMSCs protect the retina of diabetic rats by upregulating APN and NT-4 
      expression and downregulating hs-CRP expression in the retina.
CI  - International Journal of Ophthalmology Press.
FAU - Chen, Shan-Na
AU  - Chen SN
AD  - Clinical College of Ophthalmology, Tianjin Medical University, Tianjin 300070, 
      China.
AD  - Tianjin Eye Hospital, Tianjin Eye Institute, Tianjin Key Lab of Ophthalmology and 
      Visual Science, Tianjin 300020, China.
AD  - Xiamen Kehong Eye Hospital, Xiamen 361000, Fujian Province, China.
FAU - Xu, Zhi-Gang
AU  - Xu ZG
AD  - Clinical College of Ophthalmology, Tianjin Medical University, Tianjin 300070, 
      China.
AD  - Tianjin Eye Hospital, Tianjin Eye Institute, Tianjin Key Lab of Ophthalmology and 
      Visual Science, Tianjin 300020, China.
AD  - Department of Ophthalmology, Baoan Central Hospital, Shenzhen 518000, China.
FAU - Ma, Ying-Xue
AU  - Ma YX
AD  - Clinical College of Ophthalmology, Tianjin Medical University, Tianjin 300070, 
      China.
AD  - Tianjin Eye Hospital, Tianjin Eye Institute, Tianjin Key Lab of Ophthalmology and 
      Visual Science, Tianjin 300020, China.
AD  - Department of Ophthalmology, Tianjin First Central Hospital, Tianjin 300192, 
      China.
FAU - Chen, Song
AU  - Chen S
AD  - Department of Vitreous and Retinopathy, Tianjin Eye Hospital, Tianjin Eye 
      Institute, Tianjin Key Lab of Ophthalmology and Visual Science, Tianjin 300020, 
      China.
FAU - He, Guang-Hui
AU  - He GH
AD  - Department of Vitreous and Retinopathy, Tianjin Eye Hospital, Tianjin Eye 
      Institute, Tianjin Key Lab of Ophthalmology and Visual Science, Tianjin 300020, 
      China.
FAU - Han, Mei
AU  - Han M
AD  - Department of Ophthalmology, Tianjin Eye Hospital, Tianjin Eye Institute, Tianjin 
      Key Lab of Ophthalmology and Visual Science, Tianjin 300020, China.
FAU - Gao, Xiang
AU  - Gao X
AD  - Studying for a Doctor of Medicine Degree from Nankai University, Tianjin 300000, 
      China.
FAU - Wang, Jun-Hua
AU  - Wang JH
AD  - Department of Ophthalmology, Tianjin Eye Hospital, Tianjin Eye Institute, Tianjin 
      Key Lab of Ophthalmology and Visual Science, Tianjin 300020, China.
FAU - Wu, Bin
AU  - Wu B
AD  - Department of Ophthalmology, Tianjin Eye Hospital, Tianjin Eye Institute, Tianjin 
      Key Lab of Ophthalmology and Visual Science, Tianjin 300020, China.
FAU - Wang, Jian
AU  - Wang J
AD  - Department of Ophthalmology, Tianjin Eye Hospital, Tianjin Eye Institute, Tianjin 
      Key Lab of Ophthalmology and Visual Science, Tianjin 300020, China.
LA  - eng
PT  - Journal Article
DEP - 20211018
PL  - China
TA  - Int J Ophthalmol
JT  - International journal of ophthalmology
JID - 101553860
PMC - PMC8481991
OTO - NOTNLM
OT  - adiponectin
OT  - diabetic rats
OT  - diabetic retinopathy
OT  - high-sensitivity C-reactive protein
OT  - human umbilical cord mesenchymal stem cells
OT  - leukemia inhibitory factor
OT  - neurotrophin-4
EDAT- 2021/10/21 06:00
MHDA- 2021/10/21 06:01
PMCR- 2021/10/18
CRDT- 2021/10/20 06:46
PHST- 2021/04/21 00:00 [received]
PHST- 2021/07/07 00:00 [accepted]
PHST- 2021/10/20 06:46 [entrez]
PHST- 2021/10/21 06:00 [pubmed]
PHST- 2021/10/21 06:01 [medline]
PHST- 2021/10/18 00:00 [pmc-release]
AID - ijo-14-10-1508 [pii]
AID - 10.18240/ijo.2021.10.06 [doi]
PST - epublish
SO  - Int J Ophthalmol. 2021 Oct 18;14(10):1508-1517. doi: 10.18240/ijo.2021.10.06. 
      eCollection 2021.