PMID- 34673465
OWN - NLM
STAT- MEDLINE
DCOM- 20211101
LR  - 20211101
IS  - 1879-1514 (Electronic)
IS  - 0166-445X (Linking)
VI  - 240
DP  - 2021 Nov
TI  - Developmental titanium dioxide nanoparticle exposure induces oxidative stress and 
      neurobehavioral changes in zebrafish.
PG  - 105990
LID - S0166-445X(21)00249-6 [pii]
LID - 10.1016/j.aquatox.2021.105990 [doi]
AB  - The widespread commercial application of titanium dioxide nanoparticles (TiO(2) 
      NPs) leads to ubiquitous presence of TiO(2) NPs in the aquatic environment, which 
      highlights the necessity to determine their potential adverse effects on aquatic 
      organisms. The developing nerve system is particularly susceptible to environment 
      perturbation. However, few studies have explored the developmental 
      neurobehavioral toxicity of TiO(2) NPs, especially at smaller particle size 
      ranges (</=20 nm) that have relatively longer retention time in the water column. 
      In this study, zebrafish embryos were exposed to non-teratogenic concentrations 
      of 0.1 and 1 mg/L TiO(2) NPs (average size of 14-20 nm) from 8 to 108 h 
      post-fertilization (hpf) followed by various assessments at different time points 
      up to 12 days post-fertilization (dpf). Our findings revealed that 1 mg/L TiO(2) 
      NPs perturbed the motor and social behaviors in larval zebrafish. These 
      behavioral changes were characterized by decreased swimming speed in a locomotor 
      response test at 5 dpf, increased travel distance in a flash stimulus test at 
      5 dpf, increased preference to the light zone in a light/dark preference test at 
      10 dpf, and increased mirror attack and percent time spent in the mirror zone in 
      a mirror stimulus response assay at 12 dpf. Mechanistic examinations at 5 dpf 
      revealed elevated cell apoptosis and oxidative stress. Cell apoptosis was 
      characterized by increased acridine orange (AO) positive cells in the olfactory 
      region and neuromasts of the lateral line system. Oxidative stress was 
      characterized by increased lipid peroxidation, increased ROS production, and 
      upregulated catalase (cat) gene expression. In addition, TiO(2) NP exposure also 
      upregulated genes associated with the developmental nervous system such as the 
      growth associated protein 43 (gap43) and proliferating cell nuclear antigen 
      (pcna). Our results suggest that the neurobehavioral changes in larvae exposed to 
      1 mg/L TiO(2) NPs during early development may result from cell apoptosis and 
      oxidative stress induced neuronal damages.
CI  - Copyright (c) 2021. Published by Elsevier B.V.
FAU - Chen, Jiangfei
AU  - Chen J
AD  - Institute of Environmental Safety and Human Health, Wenzhou Medical University, 
      Wenzhou 325035, China. Electronic address: chenjiangfei456@126.com.
FAU - Lei, Lei
AU  - Lei L
AD  - Institute of Environmental Safety and Human Health, Wenzhou Medical University, 
      Wenzhou 325035, China.
FAU - Mo, Wen
AU  - Mo W
AD  - Zhejiang Rehabilitation Medical Center, Hangzhou 310051, China.
FAU - Dong, Haojia
AU  - Dong H
AD  - Institute of Environmental Safety and Human Health, Wenzhou Medical University, 
      Wenzhou 325035, China.
FAU - Li, Jiani
AU  - Li J
AD  - Institute of Environmental Safety and Human Health, Wenzhou Medical University, 
      Wenzhou 325035, China.
FAU - Bai, Chenglian
AU  - Bai C
AD  - Institute of Environmental Safety and Human Health, Wenzhou Medical University, 
      Wenzhou 325035, China.
FAU - Huang, Kaiyu
AU  - Huang K
AD  - The Second Affiliated Hospital and Yuying Children's Hospital, Wenzhou Medical 
      University, Wenzhou 325035, China.
FAU - Truong, Lisa
AU  - Truong L
AD  - Department of Environmental & Molecular Toxicology, Sinnhuber Aquatic Research 
      Laboratory, Oregon State University, 28645 East Highway 34, Corvallis, OR 97333, 
      United States.
FAU - Tanguay, Robyn L
AU  - Tanguay RL
AD  - Department of Environmental & Molecular Toxicology, Sinnhuber Aquatic Research 
      Laboratory, Oregon State University, 28645 East Highway 34, Corvallis, OR 97333, 
      United States.
FAU - Dong, Qiaoxiang
AU  - Dong Q
AD  - Institute of Environmental Safety and Human Health, Wenzhou Medical University, 
      Wenzhou 325035, China; The Second Affiliated Hospital and Yuying Children's 
      Hospital, Wenzhou Medical University, Wenzhou 325035, China.
FAU - Huang, Changjiang
AU  - Huang C
AD  - Institute of Environmental Safety and Human Health, Wenzhou Medical University, 
      Wenzhou 325035, China. Electronic address: cjhuang5711@163.com.
LA  - eng
PT  - Journal Article
DEP - 20211008
PL  - Netherlands
TA  - Aquat Toxicol
JT  - Aquatic toxicology (Amsterdam, Netherlands)
JID - 8500246
RN  - 0 (Water Pollutants, Chemical)
RN  - 15FIX9V2JP (titanium dioxide)
RN  - D1JT611TNE (Titanium)
SB  - IM
MH  - Animals
MH  - *Nanoparticles/toxicity
MH  - Oxidative Stress
MH  - Titanium/toxicity
MH  - *Water Pollutants, Chemical/toxicity
MH  - Zebrafish
OTO - NOTNLM
OT  - Behaviors
OT  - Oxidative stress
OT  - Titanium dioxide nanoparticles
OT  - Zebrafish
EDAT- 2021/10/22 06:00
MHDA- 2021/11/03 06:00
CRDT- 2021/10/21 21:05
PHST- 2021/01/27 00:00 [received]
PHST- 2021/09/01 00:00 [revised]
PHST- 2021/10/02 00:00 [accepted]
PHST- 2021/10/22 06:00 [pubmed]
PHST- 2021/11/03 06:00 [medline]
PHST- 2021/10/21 21:05 [entrez]
AID - S0166-445X(21)00249-6 [pii]
AID - 10.1016/j.aquatox.2021.105990 [doi]
PST - ppublish
SO  - Aquat Toxicol. 2021 Nov;240:105990. doi: 10.1016/j.aquatox.2021.105990. Epub 2021 
      Oct 8.