PMID- 34673590
OWN - NLM
STAT- MEDLINE
DCOM- 20220201
LR  - 20220427
IS  - 0974-5130 (Electronic)
IS  - 0377-4929 (Linking)
VI  - 64
IP  - 4
DP  - 2021 Oct-Dec
TI  - Expression of TLE1, INI1, beta-catenin, Claudin1, CK7, CK19, SS18 and calponin in 
      synovial sarcoma.
PG  - 707-716
LID - 10.4103/IJPM.IJPM_1137_20 [doi]
AB  - BACKGROUND AND OBJECTIVES: Synovial sarcomas (SS) are enigmatic soft tissue 
      tumors, which are yet to have a defined cell of origin. SS have a variety of 
      differential diagnosis depending upon the age of the patient and the site of 
      presentation. This makes diagnosis cumbersome unless the specific fusion SS18:SSX 
      is identified by reverse transcription-polymerase chain reaction (RT-PCR) or 
      fluorescence in situ hybridization (FISH). Immunohistochemistry is a useful tool 
      in resource-poor settings in helping to narrow the differentials and help 
      diagnose this tumor. This study set about assessing possible candidate 
      immunohistochemical markers in their utility to recognize SS. METHODS: Forty 
      cases of SS, proven by FISH were included. A tissue microarray (TMA) was 
      constructed, and immunohistochemistry was done using antibodies - TLE1 (OTI1F5), 
      beta-catenin (14), INI1 (MRQ-27), CK7 (OV-TL), CK19 (polyclonal), SS18 (polyclonal), 
      calponin (CALP), and claudin1 (Polyclonal). The expression was analyzed to arrive 
      at sensitivity and specificity. RESULTS: TLE1 had a sensitivity of 92.5% and a 
      specificity of 100%; beta-Catenin had a sensitivity of 17.5% and specificity of 
      100%; Calponin had a sensitivity of 97.5% and a specificity of 81.25%; SS18 had a 
      sensitivity of 95% and specificity of 100%; INI1 had a sensitivity of 95% and 
      specificity of 100%; CK7 had a sensitivity of 90% and specificity of 87.5%; CK19 
      had a sensitivity of 90% and a specificity of 59.38%; and Claudin had a 
      sensitivity of 85% and a specificity of 78.12%. INTERPRETATION AND CONCLUSIONS: 
      The study showed both TLE1 and SS18 are robust diagnostic markers of synovial 
      sarcoma with a sensitivity of 92% and 95%, respectively. INI1 can be used to 
      discriminate SS from nonepithelioid and nonrhabdoid differentials. Calponin 
      expression is helpful to differentiate poorly differentiated SS from its mimics. 
      CK7 is a better marker than CK19 and can be used as a replacement for EMA in the 
      initial screening panel. The use of claudin1 was restricted to delineating the 
      epithelial component. beta-Catenin had poor sensitivity, restricting its utility in 
      SS.
FAU - Madakshira, Manoj Gopal
AU  - Madakshira MG
AD  - Department of Histopathology, Post Graduate Institute of Medical Education and 
      Research, Chandigarh, India.
FAU - Radotra, Bishan Dass
AU  - Radotra BD
AD  - Department of Histopathology, Post Graduate Institute of Medical Education and 
      Research, Chandigarh, India.
FAU - Kaman, Lileswar
AU  - Kaman L
AD  - Department of General Surgery, Post Graduate Institute of Medical Education and 
      Research, Chandigarh, India.
FAU - Saikia, Uma Nahar
AU  - Saikia UN
AD  - Department of Histopathology, Post Graduate Institute of Medical Education and 
      Research, Chandigarh, India.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - India
TA  - Indian J Pathol Microbiol
JT  - Indian journal of pathology & microbiology
JID - 7605904
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Calcium-Binding Proteins)
RN  - 0 (Co-Repressor Proteins)
RN  - 0 (Microfilament Proteins)
RN  - 0 (beta Catenin)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Age Factors
MH  - Aged
MH  - Aged, 80 and over
MH  - Biomarkers, Tumor/genetics/metabolism
MH  - Calcium-Binding Proteins/*genetics
MH  - Child
MH  - Co-Repressor Proteins/*genetics/metabolism
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Male
MH  - Microfilament Proteins/*genetics/metabolism
MH  - Middle Aged
MH  - Prospective Studies
MH  - Retrospective Studies
MH  - Sarcoma/diagnosis/genetics/physiopathology
MH  - Sarcoma, Synovial/*diagnosis/*genetics/*physiopathology
MH  - Young Adult
MH  - beta Catenin/*genetics/metabolism
OTO - NOTNLM
OT  - Beta-catenin
OT  - CK19
OT  - CK7
OT  - Claudin1
OT  - INI1
OT  - SS18
OT  - TLE1
OT  - calponin
OT  - immunohistochemistry
OT  - synovial sarcoma
EDAT- 2021/10/22 06:00
MHDA- 2022/02/02 06:00
CRDT- 2021/10/21 21:10
PHST- 2021/10/21 21:10 [entrez]
PHST- 2021/10/22 06:00 [pubmed]
PHST- 2022/02/02 06:00 [medline]
AID - IndianJPatholMicrobiol_2021_64_4_707_328519 [pii]
AID - 10.4103/IJPM.IJPM_1137_20 [doi]
PST - ppublish
SO  - Indian J Pathol Microbiol. 2021 Oct-Dec;64(4):707-716. doi: 
      10.4103/IJPM.IJPM_1137_20.