PMID- 34678331
OWN - NLM
STAT- MEDLINE
DCOM- 20220225
LR  - 20220225
IS  - 1872-9096 (Electronic)
IS  - 0166-3542 (Linking)
VI  - 195
DP  - 2021 Nov
TI  - Inhibition of endocytosis of porcine reproductive and respiratory syndrome virus 
      by rottlerin and its potential prophylactic administration in piglets.
PG  - 105191
LID - S0166-3542(21)00181-9 [pii]
LID - 10.1016/j.antiviral.2021.105191 [doi]
AB  - Owing to several limitations of porcine reproductive and respiratory syndrome 
      virus (PRRSV) control procedures, the importance of antiviral agents is 
      increasing; however, limited studies have been done on the development of 
      anti-PRRSV agents. Herein, we explored the antiviral effect and mechanism of 
      rottlerin against PRRSV. We demonstrated that treatment of rottlerin at an early 
      stage of PRRSV infection significantly inhibited the viral replication. PRRSV 
      infection induced protein kinase C-delta phosphorylation, which was specifically 
      downregulated by rottlerin. The treatment of rottlerin led to disrupting the 
      PRRSV entry pathway by blocking endocytosis of the virions. Further, to evaluate 
      the anti-PRRSV effect of the rottlerin in vivo, we administrated rottlerin loaded 
      liposome to pigs infected with PRRSV LMY or FL12 strain. The treatment of 
      rottlerin-liposome reduced the blood viral load, interstitial pneumonia and 
      clinical scores compared to untreated pigs. These results provide an evidence of 
      anti-PRRSV effect of rottlerin in vitro via inhibiting PRRSV internalization and 
      in vivo, all of which strongly suggest the applicability of rottlerin as a 
      potential PRRSV prophylactic treatment.
CI  - Copyright (c) 2021. Published by Elsevier B.V.
FAU - Kang, Yeong-Lim
AU  - Kang YL
AD  - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk 
      University, Seoul, 05029, Republic of Korea.
FAU - Oh, Changin
AU  - Oh C
AD  - Department of Genetics, Yale University School of Medicine, New Haven, CT, 
      06520-8005, USA.
FAU - Ahn, So-Hyun
AU  - Ahn SH
AD  - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk 
      University, Seoul, 05029, Republic of Korea.
FAU - Choi, Jong-Chul
AU  - Choi JC
AD  - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk 
      University, Seoul, 05029, Republic of Korea.
FAU - Choi, Hwi-Yeon
AU  - Choi HY
AD  - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk 
      University, Seoul, 05029, Republic of Korea.
FAU - Lee, Sang-Won
AU  - Lee SW
AD  - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk 
      University, Seoul, 05029, Republic of Korea.
FAU - Choi, In-Soo
AU  - Choi IS
AD  - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk 
      University, Seoul, 05029, Republic of Korea.
FAU - Song, Chang-Seon
AU  - Song CS
AD  - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk 
      University, Seoul, 05029, Republic of Korea.
FAU - Lee, Joong-Bok
AU  - Lee JB
AD  - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk 
      University, Seoul, 05029, Republic of Korea.
FAU - Park, Seung-Yong
AU  - Park SY
AD  - Laboratory of Infectious Diseases, College of Veterinary Medicine, Konkuk 
      University, Seoul, 05029, Republic of Korea. Electronic address: 
      paseyo@konkuk.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20211020
PL  - Netherlands
TA  - Antiviral Res
JT  - Antiviral research
JID - 8109699
RN  - 0 (Acetophenones)
RN  - 0 (Antiviral Agents)
RN  - 0 (Benzopyrans)
RN  - E29LP3ZMUH (rottlerin)
SB  - IM
MH  - Acetophenones/*pharmacology
MH  - Animals
MH  - Antiviral Agents/*pharmacology
MH  - Benzopyrans/*pharmacology
MH  - Cell Line
MH  - Endocytosis/*drug effects
MH  - Lung Diseases, Interstitial/prevention & control
MH  - Porcine Reproductive and Respiratory Syndrome/*drug therapy/pathology
MH  - Porcine respiratory and reproductive syndrome virus/*drug effects
MH  - Swine
MH  - Viral Load/drug effects
MH  - Virus Internalization/drug effects
MH  - Virus Replication/drug effects
OTO - NOTNLM
OT  - Antivirals
OT  - Liposome
OT  - PRRSV
OT  - Protein kinase C
OT  - Rottlerin
EDAT- 2021/10/23 06:00
MHDA- 2022/02/26 06:00
CRDT- 2021/10/22 20:31
PHST- 2021/05/15 00:00 [received]
PHST- 2021/08/27 00:00 [revised]
PHST- 2021/10/18 00:00 [accepted]
PHST- 2021/10/23 06:00 [pubmed]
PHST- 2022/02/26 06:00 [medline]
PHST- 2021/10/22 20:31 [entrez]
AID - S0166-3542(21)00181-9 [pii]
AID - 10.1016/j.antiviral.2021.105191 [doi]
PST - ppublish
SO  - Antiviral Res. 2021 Nov;195:105191. doi: 10.1016/j.antiviral.2021.105191. Epub 
      2021 Oct 20.