PMID- 34680659
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20211026
IS  - 2075-4450 (Print)
IS  - 2075-4450 (Electronic)
IS  - 2075-4450 (Linking)
VI  - 12
IP  - 10
DP  - 2021 Sep 30
TI  - Pathogenicity Detection and Genome Analysis of Two Different Geographic Strains 
      of BmNPV.
LID - 10.3390/insects12100890 [doi]
LID - 890
AB  - The pathogenicity of different concentrations of Bombyx mori nuclear polyhedrosis 
      virus- Zhenjiang strain (BmNPV ZJ) and Yunnan strain (BmNPV YN) was assessed in 
      Baiyu larvae. The structures of the two viral strains were observed by 
      negative-staining electron microscopy, and their proliferation was examined by 
      quantitative polymerase chain reaction (qPCR). The genomic sequences of these two 
      viruses were obtained to investigate the differences in their pathogenicity. The 
      lethal concentration 50 (LC(50)) of BmNPV ZJ against Baiyu larvae was higher than 
      that of BmNPV YN, indicating a relatively more robust pathogenicity in BmNPV YN. 
      Electron microscopic images showed that the edges of BmNPV YN were clearer than 
      those of BmNPV ZJ. The qPCR analysis demonstrated significantly higher relative 
      expressions of immediately early 1 gene (ie-1), p143, vp39, and polyhedrin genes 
      (polh) in BmNPV ZJ than in BmNPV YN at 12-96 h. The complete genomes of BmNPV ZJ 
      and BmNPV YN were, respectively, 135,895 bp and 143,180 bp long, with 141 and 145 
      coding sequences and 40.93% and 39.71% GC content. Considering the BmNPV ZJ 
      genome as a reference, 893 SNP loci and 132 InDel mutations were observed in the 
      BmNPV YN genome, resulting in 106 differential gene sequences. Among these 
      differential genes, 76 (including 22 hub genes and 35 non-hub genes) possessed 
      amino acid mutations. Thirty genes may have been related to viral genome 
      replication and transcription and five genes may have been associated with the 
      viral oral infection. These results can help in understanding the mechanisms of 
      pathogenicity of different strains of BmNPV in silkworms.
FAU - Guo, Huimin
AU  - Guo H
AUID- ORCID: 0000-0002-8373-7600
AD  - College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 
      212018, China.
AD  - The Sericultural Research Institute, Chinese Academy of Agricultural Sciences, 
      Zhenjiang 212018, China.
FAU - Zhang, Benzheng
AU  - Zhang B
AD  - College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 
      212018, China.
FAU - Zheng, Xin
AU  - Zheng X
AD  - College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 
      212018, China.
FAU - Sun, Juan
AU  - Sun J
AD  - College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 
      212018, China.
FAU - Guo, Huiduo
AU  - Guo H
AD  - College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 
      212018, China.
FAU - Li, Gang
AU  - Li G
AD  - College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 
      212018, China.
FAU - Zhao, Guodong
AU  - Zhao G
AD  - College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 
      212018, China.
FAU - Xu, Anying
AU  - Xu A
AD  - College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 
      212018, China.
FAU - Qian, Heying
AU  - Qian H
AD  - College of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang 
      212018, China.
AD  - The Sericultural Research Institute, Chinese Academy of Agricultural Sciences, 
      Zhenjiang 212018, China.
LA  - eng
GR  - BE2020418/China Agriculture Research System of MOF and MARA and Key R & D Plan of 
      Jiangsu Province (Modern Agriculture)/
GR  - BK20181228, BK20201229/the Nutural Science Foundation of Jiangsu Province/
PT  - Journal Article
DEP - 20210930
PL  - Switzerland
TA  - Insects
JT  - Insects
JID - 101574235
PMC - PMC8537803
OTO - NOTNLM
OT  - Bombyx mori nuclear polyhedrosis virus
OT  - pathogenicity difference
OT  - qPCR
OT  - sequencing analysis
COIS- The authors declare no conflict of interest.
EDAT- 2021/10/24 06:00
MHDA- 2021/10/24 06:01
PMCR- 2021/09/30
CRDT- 2021/10/23 01:04
PHST- 2021/07/28 00:00 [received]
PHST- 2021/09/26 00:00 [revised]
PHST- 2021/09/27 00:00 [accepted]
PHST- 2021/10/23 01:04 [entrez]
PHST- 2021/10/24 06:00 [pubmed]
PHST- 2021/10/24 06:01 [medline]
PHST- 2021/09/30 00:00 [pmc-release]
AID - insects12100890 [pii]
AID - insects-12-00890 [pii]
AID - 10.3390/insects12100890 [doi]
PST - epublish
SO  - Insects. 2021 Sep 30;12(10):890. doi: 10.3390/insects12100890.